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Molecular mechanisms underlying production of oocyte maturation inducing hormone in teleost

Research Project

Project/Area Number 18H02275
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionHokkaido University

Principal Investigator

Ijiri Shigeho  北海道大学, 水産科学研究院, 准教授 (90425421)

Co-Investigator(Kenkyū-buntansha) 足立 伸次  北海道大学, 水産科学研究院, 特任教授 (40231930)
柴田 安司  帝京科学大学, 生命環境学部, 准教授 (80446260)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywords卵成熟誘起ホルモン / DHP / MIH / ティラピア / ウナギ / チョウザメ / 条鰭類 / メダカ / 卵成熟誘起ステロイドホルモン / 20β-HSD / 20b-HSD / Hsd17β12L / サクラマス / ゼブラフィッシュ
Outline of Final Research Achievements

Maturation-inducing hormone in fish was identified as DHP. DHP is directly related to the maturational successful to produce fertilizable eggs. This study proved that Hsd17b12L is the enzyme to convert the precursor 17OHP to DHP throughout the fish evolution from sturgeon to eel, salmon and tilapia. Meanwhile, this study illustrated that DHP production is controlled by not only Hsd17b12L expression but also 17OHP production. Salmonid and medaka were controlled by Hsd17b12L expression, however sturgeon and eel were controlled by 17OHP production. 17OHP production is controlled by the balance of cyp17a1 and cyp17a2 expressions. This study has not reached to understand control mechanisms to regulate transcription of these genes. Toward understanding in MIS production control, transcriptional control of cyp17a1, cyp17a2 and hsd17b12L will be necessary to develop artificial maturation control for unmanageable fish species.

Academic Significance and Societal Importance of the Research Achievements

絶滅が危惧される魚種では人が増殖を助ける必要がある。その際、受精可能な卵を得るには卵成熟をホルモン操作によって人為誘導する必要がある。卵成熟を誘起するDHPが適切な時期に卵巣で作られれば卵成熟する。飼育下の繁殖困難魚ではDHPが作られず産卵に至らない。ところが、DHP産生の分子機構は古くから間違った理解が広がっていた。本研究は、従来の説を完全に否定し、条鰭類を網羅した証拠を示して正しいDHP産生制御機構を初めて明らかにしたものである。本研究で扱う、ウナギやチョウザメなどで、人為卵成熟技術を正しく評価、発展させる分子的評価基盤を世界で初めて示すことができたものである。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (10 results)

All 2022 2021 2020 2018

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Regulation of 17α-Hydroxyprogesterone Production during Induced Oocyte Maturation and Ovulation in Amur Sturgeon. (Acipenser schrenckii)2022

    • Author(s)
      Yuya Hasegawa, Ryohei Surugaya, Shinji Adachi and Shigeho Ijiri
    • Journal Title

      Journal of Marine Science and Engineering

      Volume: 10 Issue: 1 Pages: 1-22

    • DOI

      10.3390/jmse10010086

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 17β-hydroxysteroid dehydrogenase type 12-like is associated with maturation-inducing steroid synthesis during induced oocyte maturation and ovulation in sturgeons.2022

    • Author(s)
      Yuya Hasegawa, Shigeho Ijiri*, Ryohei Surugaya, Ryuichi Sakai, Shinji Adachi.
    • Journal Title

      Aquaculture

      Volume: 564 Pages: 1-12

    • DOI

      10.1016/j.aquaculture.2021.737238

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 17β-Hydroxysteroid dehydorogenase Type 12 is responsible for maturation-inducing steroid synthesis during oocyte maturation in Nile tilapia.2020

    • Author(s)
      Aranyakanont C., Ijiri S., Hasegawa Y., Adachi S.
    • Journal Title

      General and Comparative Endocrinology

      Volume: 290 Pages: 113399-113399

    • DOI

      10.1016/j.ygcen.2020.113399

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] ナイルティラピア ナイルティラピア Gsdfが性分化関連遺伝子の発現に与える影響 が性分化関連遺伝子の発現に与える影響 が性分化関連遺伝子の発現に与える影響 が性分化関連遺伝子の発現に与える影響 が性分化関連遺伝子の発現に与える影響2022

    • Author(s)
      荒井那允 ・田中桜花 ・井尻成保
    • Organizer
      令和4年度日本水産学会春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ニホンウナギcyp19a1a遺伝子のnr5a1、foxl2、FSHによる発現調節2021

    • Author(s)
      中瀬英郁・堀内萌未・井尻成保
    • Organizer
      令和3年度日本水産学会秋季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] アムールチョウザメ17β-水酸基脱水素酵素タイプ12-likeの酵素活性および卵成熟期における発現動態2021

    • Author(s)
      長谷川祐也・井尻成保・駿河谷諒平・酒井隆一・足立伸次
    • Organizer
      令和3年度日本水産学会秋季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] GFP発現ウナギ腎臓由来EK1細胞はウナギ生体内で生存できるのか2020

    • Author(s)
      地村実咲・陳顧慧・伴戸久徳・笠井久会・井尻成保・足立伸次
    • Organizer
      令和2年度日本水産学会北海道支部大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] チョウザメ類におけるhsd17b12Lの酵素活性および発現動態の解析2020

    • Author(s)
      長谷川祐也・駿河谷諒平・井尻成保・足立伸次
    • Organizer
      日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] バキュロウイルス遺伝子導入系を利用したGFP産生EK1細胞の作製2020

    • Author(s)
      陳顧慧・谷口祐介・伴戸久徳・笠井久会・井尻成保・足立伸次
    • Organizer
      日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 17β-Hydroxysteroid dehydrogenase type 12 is responsible for maturation-inducing steroid synthesis during oocyte maturation in Nile tilapia2018

    • Author(s)
      Aranyakanont, C., Ijiri S., Hasegawa, Y., Adachi, S.
    • Organizer
      11th International Symposium on Reproductive Physiology of Fish
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2023-01-30  

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