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temperature-responsive molecules in mammary epithelial cells regulate the morphogenesis and functional expression of mammary epithelial cells.

Research Project

Project/Area Number 18H02320
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 42010:Animal production science-related
Research InstitutionHokkaido University

Principal Investigator

Kobayashi Ken  北海道大学, 農学研究院, 准教授 (30449003)

Co-Investigator(Kenkyū-buntansha) 川原 学  北海道大学, 農学研究院, 准教授 (70468700)
磯部 直樹  広島大学, 統合生命科学研究科(生), 教授 (80284230)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywords乳腺上皮細胞 / 温度受容体 / TRP チャネル / ヒートショックプロテイン / 乳産生 / タイトジャンクション / 血液乳関門 / TRPチャネル / 乳分泌 / 泌乳期 / 形態形成 / 暑熱ストレス / 乳腺発達 / 乳腺 / 寒冷ストレス / 乳分泌培養モデル / 熱ショックタンパク質
Outline of Final Research Achievements

Mammary epithelial cells (MECs) develop mammary epithelial tissues during pregnancy and produce milk secretion after parturition. MECs are also exposed to active temperature changes due to changes in environmental temperature and metabolic heat associated with milk production. MECs express heat shock proteins (HSPs) and temperature responsive TRP channels. In this study, we investigated the role of HSPs and TRP channels on the MEC functions. First, we confirmed that hormones and inflammatory components changes the expression levels of the thermos responsive molecules. In addition, activation and inactivation of the thermos responsive molecules influenced milk production and tight junction barrier. Furthermore, local heat treatment on goat udder affected milk composition and somatic cell counts. These things suggest that activation and inactivation of HSPs and TRP channels expressed in MECs regulate mammary epithelial development during pregnancy and milk production during lactation.

Academic Significance and Societal Importance of the Research Achievements

乳牛の乳生産性向上は酪農産業の発展に必要不可欠な課題である。従来、暑熱ストレスや寒冷ストレスに伴う乳牛の乳生産性の低下に関する研究は飼養管理や個体レベルの生理学的なアプローチで進められてきた。本研究の結果、乳腺上皮細胞には温度変化に応答性のある分子群が発現し、乳腺上皮細胞は細胞周囲の温度変化をこれらの温度応答性分子群を介して感知することによって、乳産生能力を変化させることが新たに見出された。したがって、本研究の成果は分子レベルで温度変化と乳生産性の因果関係を示したものであり、従来の畜産領域の研究成果と分子細胞生物学に基づく研究成果を橋渡しするものと判断される。

Report

(4 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (14 results)

All 2021 2020 2019 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 1 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] Lipopolysaccharide and lipoteichoic acid influence milk production ability via different early responses in bovine mammary epithelial cells.2021

    • Author(s)
      Tsugami Y, Wakasa H, Kawahara M, Nishimura T, Kobayashi K
    • Journal Title

      Experimental cell Research

      Volume: 15 Issue: 2 Pages: 112472-112472

    • DOI

      10.1016/j.yexcr.2021.112472

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adverse effects of LPS on membrane proteins in lactating bovine mammary epithelial cells.2021

    • Author(s)
      Tsugami Y, Wakasa H, Kawahara M, Watanabe A, Suzuki T, Nishimura T, Kobayashi K
    • Journal Title

      Cell and Tissue Research

      Volume: ahead of print Issue: 2 Pages: 435-448

    • DOI

      10.1007/s00441-020-03344-0

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suppressive effects of curcumin on milk production without inflammatory responses in lactating mammary epithelial cells.2021

    • Author(s)
      Kobayashi K, Tsugami Y, Suzuki N, Suzuki T, Nishimura T
    • Journal Title

      Phytomedicine

      Volume: 80 Pages: 153360-153360

    • DOI

      10.1016/j.phymed.2020.153360

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Menthol from Mentha piperita suppresses the milk production of lactating mammary epithelial cells in vivo and in vitro.2020

    • Author(s)
      Suzuki N, Tsugami Y, Wakasa H, Suzuki T, Nishimura T, Kobayashi K
    • Journal Title

      Molecular Nutrition and Food Research

      Volume: 14 Issue: 24

    • DOI

      10.1002/mnfr.202000853

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct expression patterns of fibrillar collagen types I, III, and V in association with mammary gland remodeling during pregnancy, lactation and weaning.2020

    • Author(s)
      Tsutsui S, Wakasa H, Tsugami Y, Suzuki T, Nishimura T, Kobayashi K
    • Journal Title

      Journal of Mammary Gland Biology and Neoplasia

      Volume: 25 Issue: 3 Pages: 219-219

    • DOI

      10.1007/s10911-020-09457-0

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of an in vitro culture model to study milk production and the blood-milk barrier with bovine mammary epithelial cells.2020

    • Author(s)
      Tsugami Y, Suzuki N, Kawahara M, Suzuki T, Nishimura T, Kobayashi K
    • Journal Title

      Animal Science Journal

      Volume: 91 Issue: 1 Pages: 1-12

    • DOI

      10.1111/asj.13355

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adverse effects of coumestrol and genistein on mammary morphogenesis and future milk production ability of mammary epithelial cells.2020

    • Author(s)
      Kumai A, Tsugami Y, Suzuki N, Suzuki T, Nishimura T, Kobayashi K
    • Journal Title

      Advanced Biosystems

      Volume: in press Issue: 4 Pages: 1900187-1900187

    • DOI

      10.1002/adbi.201900187

    • NAID

      120007000860

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct expression patterns of aquaporin 3 and 5 in ductal and alveolar epithelial cells in mouse mammary glands before and after parturition.2020

    • Author(s)
      Kaihoko Y, Tsugami Y, Suzuki N, Suzuki T, Nishimura T, Kobayashi K
    • Journal Title

      Cell and Tissue Research

      Volume: in press Issue: 3 Pages: 513-526

    • DOI

      10.1007/s00441-020-03168-y

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nicotine directly affects milk production in lactating mammary epithelial cells concurrently with inactivation of STAT5 and glucocorticoid receptor in vitro.2020

    • Author(s)
      Kobayashi K, Tsugami Y, Suzuki N, Suzuki T, Nishimura T
    • Journal Title

      Toxicology in Vitro

      Volume: 63 Issue: 2 Pages: 104741-104741

    • DOI

      10.1016/j.yexcr.2018.06.038

    • NAID

      120006980036

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] 暑熱ストレス下の高温状態が乳腺上皮細胞の細胞内シグナル経路と乳腺胞形成に及ぼす影響のin vitro検証2021

    • Author(s)
      若狹 遥、 津上 優作、 草野 楓也、 西邑 隆徳、磯部直樹、小林 謙
    • Organizer
      日本畜産学会第128回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ヤギ乳房局所のメントール塗布が抗菌因子産生に及ぼす影響2021

    • Author(s)
      大野瑠璃、新居隆浩、吉村幸則、小林謙、磯部直樹
    • Organizer
      日本畜産学会第128回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] クルクミンは乳腺上皮細胞に対して影響力の大きい生理活性物質である2019

    • Author(s)
      小林 謙, 津上優作, 隈井 仰, 鈴木徳宏, 鈴木貴弘, 西邑隆徳
    • Organizer
      第24回日本乳房炎研究会・学術集会
    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
  • [Presentation] リポ多糖とリポタイコ酸がウシ乳腺上皮細胞の乳産生とタイトジャンクションに及ぼす影響2019

    • Author(s)
      津上優作, 隈井 仰, 鈴木徳宏, 鈴木貴弘, 西邑隆徳, 小林 謙
    • Organizer
      第24回日本乳房炎研究会・学術集会
    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
  • [Remarks] 細胞組織生物学研究室

    • URL

      http://lab.agr.hokudai.ac.jp/cell_tissue_biology/

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

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