Development of new rumen immunology by elucidating the interrelationship between rumen tissue and host immune system

• Project/Area Number: 18H02325
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 42010:Animal production science-related
• Research Institution: Tohoku University
• Principal Investigator: ROH SANGGUN 東北大学, 農学研究科, 准教授 (90322130)
• Co-Investigator(Kenkyū-buntansha): 芳賀 聡 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門, 主任研究員 (90442748)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 反芻動物 / 反芻胃 / ホメオスタシス / ウシ / ルーメン / 恒常性 / 免疫 / 亜急性ルーメンアシドーシス

Outline of Final Research Achievements

This study was to clarify the interaction between the immune response, homeostasis, and the host immune system that occur in rumen epithelium. The results are as follows:(1) CCL5 elevated after weaning is thought to induce monocyte migration and differentiation into macrophages, and the CCL5-monocyte / macrophage axis may play an important role in maintaining homeostasis in rumen epithelium. (2) In the rumen epithelium, IL-1β contributes to the maintenance of homeostasis when SCFA, LPS, flagellin, etc. increase in the rumen due to weaning and the environment by the environment changes in rumen, and in addition it is suggested to contribute to the repair of damage by promoting cell proliferation.

Academic Significance and Societal Importance of the Research Achievements

本研究成果により、ルーメン免疫学を視点としたルーメン内微生物、ルーメン絨毛組織と宿主免疫系の相互関係を解明し、畜産学・獣医学領域におけるルーメン免疫応答と恒常性維持を考慮した飼料栄養、飼育管理方法とルーメン柔毛損傷治療法を開発するために必要な基盤技術を構築することができた。

Research Products

• [Int'l Joint Research] Konkuk University(韓国)
• [Int'l Joint Research] Seoul National University(韓国)
• [Int'l Joint Research] Seoul National University(韓国)
• [Journal Article] Ruminal epithelial insulin-like growth factor-binding proteins 2, 3, and 6 are associated with epithelial cell proliferation (2020)
  • Author(s): Nishihara, K., Suzuki, Y., Roh, S.
  • Journal Title: Animal Science Journal Volume: 91 Issue: 1
  • DOI: 10.1111/asj.13422
  • Peer Reviewed
• [Journal Article] Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef Calves (2020)
  • Author(s): Kim, W. S., Ghassemi Nejad, J., Roh, S.m Lee, H. G.
  • Journal Title: Animals Volume: 10 Issue: 5 Pages: 895-895
  • DOI: 10.3390/ani10050895
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Growth of rumen papillae in weaned calves is associated with lower expression of insulin-like growth factor-binding proteins 2, 3, and 6 (2019)
  • Author(s): Nishihara, K., Suzuki, Y., Kim, D., Roh SG
  • Journal Title: Animal Science Journal Volume: 90 Issue: 9 Pages: 1289-1292
  • DOI: 10.1111/asj.13270
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] ルーメン上皮の恒常性維持機構 (2019)
  • Author(s): 西原昂来、盧 尚建
  • Journal Title: 栄養生理研究会報 Volume: 63(2) Pages: 27-34
  • NAID: 40022023159
  • Peer Reviewed / Open Access
• [Journal Article] Comparative transcriptome analysis of rumen papillae in suckling and weaned Japanese Black calves using RNA sequencing (2018)
  • Author(s): Nishihara K, Kato D, Suzuki Y, Kim D, Nakano M, Yajima Y, Haga S, Nakano M, Ishizaki H, Kawahara-Miki R, Kono T, Katoh K, Roh S
  • Journal Title: Journal of Animal Science Volume: 96 Issue: 6 Pages: 2226-2237
  • DOI: 10.1093/jas/skx016
  • Peer Reviewed
• [Journal Article] Downregulated Angiopoietin-like Peptide 8 Production at Calving Related to Changes in Lipid Metabolism in Dairy Cows (2018)
  • Author(s): 2.Nakano M, Suzuki Y, Haga S, Yamauchi E, Kim D, Nishihara K, Nakajima K, Gotoh T, Park S, Baik M, Katoh K, Roh SG
  • Journal Title: Journal of Animal Science Volume: 96(7) Issue: 7 Pages: 2646-2658
  • DOI: 10.1093/jas/sky162
  • Int'l Joint Research
• [Presentation] Roles of Toll-like receptor 5 ligand in rumen epithelium (2019)
  • Author(s): Nishihara K, Roh S
  • Organizer: 3rd International Symposium on Immunology and Biotechnology
  • Int'l Joint Research / Invited
• [Presentation] Antimicrobial peptide in rumen epithelium (2019)
  • Author(s): Roh S
  • Organizer: 4th International Animal Science and Biotechnology Forum
  • Int'l Joint Research / Invited
• [Presentation] The control of homeostasis in rumen epithelium of Japanese Black cattle (2019)
  • Author(s): Roh S
  • Organizer: 18th Chulalongkorn University Veterinary Conference.
  • Int'l Joint Research / Invited
• [Presentation] A study on Relationship of Blood Metabolites and Carcass Traits in Hanwoo Steers. (2019)
  • Author(s): Kim MJ, Son GH, Roh S, Ahn JS, Park JK, Park BK, Shin JS.
  • Organizer: International symposium on ruminant physiology 2019
  • Int'l Joint Research
• [Presentation] Roles of Toll-like receptor 5 ligand in the innate immune system in primary bovine rumen epithelial cells (2019)
  • Author(s): Nishihara K, Roh SG.
  • Organizer: International Symposium on Ruminant Physiology 2019
  • Int'l Joint Research / Invited
• [Presentation] The control of homeostasis in rumen epithelium of Japanese Black cattle (2018)
  • Author(s): Roh SG, Nishihara K
  • Organizer: The 2018 ASAS-CSAS Annual Meeting
  • Int'l Joint Research / Invited
• [Presentation] CCL5-monocyte/macrophage axis maintains homeostasis in rumen epithelium (2018)
  • Author(s): Nishihara K, Haga S and Roh SG
  • Organizer: The 2018 ASAS-CSAS Annual Meeting
  • Int'l Joint Research