| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Outline of Final Research Achievements |
In this study, we developed a method named PACE to quantitatively analyze the effect of codons on mRNA stability (codon effect) in early zebrafish embryos. Using the PACE method, we revealed that the codon effect correlates with the amount of tRNA corresponding to the codons and the translation rate by the ribosome. We also experimentally demonstrated that changes in the amount of tRNA lead to changes in the corresponding codon effect. Furthermore, we demonstrated that the mRNA degradation mechanism by the codon effect acts independently of the no-go decay pathway, which is a translational quality control mechanism by the ribosome.
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