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Elucidation of genetic program controlling cerebellar neural circuit formation

Research Project

Project/Area Number 18H02448
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44020:Developmental biology-related
Research InstitutionNagoya University

Principal Investigator

Hibi Masahiko  名古屋大学, 理学研究科, 教授 (40273627)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords発生・分化 / 神経科学 / 脳・神経 / 遺伝学 / 遺伝子
Outline of Final Research Achievements

Neurons in functional neural circuits are generated from neural stem cells or neural progenitor cells during development. However, the mechanisms of differentiation from these cells into individual types of neurons that form complex neural circuit structures is still largely unknown. In this study, we focused on cerebellar Purkinje cells and their input cells, the neurons in the inferior olivary nucleus, which constitute cerebellar neural circuits, and elucidated part of the genetic program that controls the differentiation of these neurons and the formation of neural circuits.

Academic Significance and Societal Importance of the Research Achievements

動物の複雑な行動を制御する神経回路は遺伝プログラムに従って形成される。本研究では、小脳神経回路をモデルとして、小脳神経回路を構成するプルキンエ細胞と下オリーブ核ニューロンの、細胞分化や神経回路形成を制御する遺伝プログラムの一端を解明した。本研究は、多様な神経細胞が産生される発生生物学のメカニズムを明らかにするだけでなく、小脳神経回路の異常によって引き起こされる種々のヒト疾患の病態解明や治療法の開発にも貢献する可能性がある。

Report

(4 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (33 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (13 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 13 results,  Open Access: 9 results) Presentation (19 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results)

  • [Int'l Joint Research] University of South Carolina(米国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Morphological analysis of the cerebellum and its efferent system in a basal actinopterygian fish, Polypterus senegalus2022

    • Author(s)
      Ikenaga Takanori、Shimomai Rinko、Hagio Hanako、Kimura Satoru、Matsumoto Kazumasa、Kato Dai‐ichiro、Uesugi Kentaro、Takeuchi Akihisa、Yamamoto Naoyuki、Hibi Masahiko
    • Journal Title

      Journal of Comparative Neurology

      Volume: 530 Issue: 8 Pages: 1231-1246

    • DOI

      10.1002/cne.25271

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of Cerebellar Neural Circuits in Active Avoidance Conditioning in Zebrafish2021

    • Author(s)
      Wataru Koyama, Ryo Hosomi, Koji Matsuda, Koichi Kawakami, Masahiko Hibi, Takashi Shimizu
    • Journal Title

      eNeuro

      Volume: 8 Issue: 3 Pages: 0507-0520

    • DOI

      10.1523/eneuro.0507-20.2021

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cfdp1 controls the cell cycle and neural differentiation in the zebrafish cerebellum and retina2021

    • Author(s)
      Tsubasa Itoh, Shinsuke Inoue, Xiaoding Sun, Ryo Kusuda, Masahiko Hibi, Takashi Shimizu
    • Journal Title

      Developmental Dynamics

      Volume: 250 Issue: 11 Pages: 1618-1633

    • DOI

      10.1002/dvdy.371

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Maintenance of quiescent oocytes by noradrenergic signals2021

    • Author(s)
      Jeongho Kim, Moonjung Hyun, Masahiko Hibi, Young-Jai You
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 6925-6925

    • DOI

      10.1038/s41467-021-26945-x

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular properties of new enzyme rhodopsins with PDE activity2020

    • Author(s)
      Sugiura M., Tsunoda SP., Hibi M., Kandori H
    • Journal Title

      ACS Omega

      Volume: 5 Issue: 18 Pages: 10602-10609

    • DOI

      10.1021/acsomega.0c01113

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functionally distinct Purkinje cell types show temporal precision in encoding locomotion2020

    • Author(s)
      Weipang Chang, Andrea Pedroni, Victoria Hohendorf, Stefania Giacomello, Masahiko Hibi, Reinhard W. Koster, Konstantinos Ampatzis
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 117 Issue: 29 Pages: 17330-17337

    • DOI

      10.1073/pnas.2005633117

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Gsx2 is required for specification of neurons in the inferior olivary nuclei from Ptf1a-expressing neural progenitors in zebrafish.2020

    • Author(s)
      Itoh T, Takeuchi M, Sakgami M, Asakawa K, Sumiyama K, Kawakami K, Shimizu T, Hibi M.
    • Journal Title

      Development

      Volume: 147

    • DOI

      10.1242/dev.190603

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Syntaphilin-Mediated Docking of Mitochondria at the Growth Cone Is Dispensable for Axon Elongation In Vivo2019

    • Author(s)
      Tine Verreet , Cory J Weaver , Hiromu Hino , Masahiko Hibi, Fabienne E Poulain
    • Journal Title

      eNeuro

      Volume: 6 Issue: 5 Pages: ENEURO.0026-19.2019

    • DOI

      10.1523/eneuro.0026-19.2019

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish.2019

    • Author(s)
      Nimura T, Itoh T, Hagio H, Hayashi T, Di Donato V, Takeuchi M, Itoh T, Inoguchi F, Sato Y, Yamamoto N, Katsuyama Y, Del Bene F, Shimizu T, Hibi M.
    • Journal Title

      Dev Biol.

      Volume: 455 Issue: 2 Pages: 393-408

    • DOI

      10.1016/j.ydbio.2019.07.010

    • NAID

      120006808762

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Tracing of Afferent Connections in the Zebrafish Cerebellum Using Recombinant Rabies Virus2019

    • Author(s)
      Dohaku Ryuji、Yamaguchi Masahiro、Yamamoto Naoyuki、Shimizu Takashi、Osakada Fumitaka、Hibi Masahiko
    • Journal Title

      Frontiers in Neural Circuits

      Volume: 13 Pages: 1-15

    • DOI

      10.3389/fncir.2019.00030

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Color opponency with a single kind of bistable opsin in the zebrafish pineal organ2018

    • Author(s)
      Wada Seiji、Shen Baoguo、Kawano-Yamashita Emi、Nagata Takashi、Hibi Masahiko、Tamotsu Satoshi、Koyanagi Mitsumasa、Terakita Akihisa
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 115 Issue: 44 Pages: 11310-11315

    • DOI

      10.1073/pnas.1802592115

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Madagascar ground gecko genome analysis characterizes asymmetric fates of duplicated genes2018

    • Author(s)
      Hara Y, Takeuchi M, Kageyama Y, Tatsumi K, Hibi M, Kiyonari H, Kuraku S.
    • Journal Title

      BMC Biology

      Volume: 16 Issue: 1 Pages: 40-40

    • DOI

      10.1186/s12915-018-0509-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish.2018

    • Author(s)
      Nagao Y, Takada H, Miyadai M, Adachi T, Seki R, Kamei Y, Hara I, Taniguchi Y, Naruse K, Hibi M, Kelsh RN, Hashimoto H
    • Journal Title

      PLoS Genetics

      Volume: 14(4) Issue: 4 Pages: 1007260-1007260

    • DOI

      10.1371/journal.pgen.1007260

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Role of transcription factors Skor1b/2 and Foxp1b/4 in differentiation of Purkinje cells in zebrafish2021

    • Author(s)
      Itoh, T., Yura, S., Nakanishi, A., Uehara, M., Shimizu, T., Hibi, M.
    • Organizer
      第54回 日本発生生物学学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Molecular mechanisms of Purkinje cell differentiation mediated by transcriptional regulators2021

    • Author(s)
      Uehara, M., Itoh., T., Yura, S., Nakanishi, A., Shimizu, T., Hibi, M.
    • Organizer
      The 27th Japanese Medaka and Zebrafish Meeting
    • Related Report
      2020 Annual Research Report
  • [Presentation] Molecular mechanisms that control differentiation of Purkinje cells in zebrafish2020

    • Author(s)
      Itoh, T., Yura, S., Nakanishi, A., Uehara, M., Shimizu, T., Hibi, M.
    • Organizer
      The 26th Japanese Medaka and Zebrafish Meeting
    • Related Report
      2020 Annual Research Report
  • [Presentation] 小型魚類を用いた小脳神経回路の形成と機能の解析2019

    • Author(s)
      日比正彦
    • Organizer
      第一回小脳システム研究会シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Generation of novel optogenetic tools controlling intracellular second messengers in neurons2019

    • Author(s)
      Hagio H, Matsuda K, Shimizu T, Nagata T, Koyanagi M, Terakita A, Hibi M
    • Organizer
      第25回小型魚類研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Differentiation of inferior olivary nucleus neurons and Purkinje cells from Ptf1a-expressing progenitors2019

    • Author(s)
      Itoh T, Yura S, Takeuchi M, Sakagami M, Shimizu T, Hibi M
    • Organizer
      第25回小型魚類研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Development and function of zebrafish cerebellar neural circuitry2019

    • Author(s)
      Hibi M, Itoh T, Yura S, Matsuda K, Shimizu T
    • Organizer
      NEURO2019(第42回日本神経科学大会、第62回日本神経化学会大会)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Development and function of zebrafish cerebellar neural circuits2019

    • Author(s)
      Hibi M, Itoh T, Yura S, Matsuda K, Shimizu T
    • Organizer
      Gordon Research Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Roles of transcriptional regulators Foxp1b/Foxp4 and Skor1b/Skor2 in differentiation of Purkinje cells in the zebrafish cerebellum2019

    • Author(s)
      Yura S, Itoh T, Shimizu T, Hibi M
    • Organizer
      第52回日本発生生物学大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Cfdp1 regulates cell cycle and differentiation of granule cells in the zebrafish cerebellum2019

    • Author(s)
      Shimizu T, Inoue S, Sun X, Hibi M
    • Organizer
      第52回日本発生生物学大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Molecular mechanisms for differentiation of the inferior olivary nucleus neurons in zebrafish2019

    • Author(s)
      Itoh T, Takeuchi M, Sakagami M, Shimizu T, Hibi M
    • Organizer
      第52回日本発生生物学大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Transcriptional regulators controlling formation of the cerebellar neural circuits.2018

    • Author(s)
      Hibi M, Itoh T, Yura S, Matsuda K, Shimizu T
    • Organizer
      5th Imaging Structure and Function in the Zebrafish Brain Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genetic control for development of the inferior olive nucleus neurons in zebrafish.2018

    • Author(s)
      Itoh T, Takeuchi M, Sakagami M, Asakawa K, Kawakami K, Shimizu T, Hibi M
    • Organizer
      13th International Zebrafish Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Involvement of axonal transport of Reelin in the layer and the neural circuit formation of the cerebellum and the optic tectum.2018

    • Author(s)
      Hibi M, Nimura T, Itoh T, Hayashi T, Takeuchi M, Di Donata V, Del Bene F, Shimizu T.
    • Organizer
      13th International Zebrafish Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The cerebellar neural circuits differentially regulate freezing and active avoidance responses in the classical fear conditioning.2018

    • Author(s)
      Matsuda K, Yoshida M, Hosomi R, Tanabe H, Kawakami K, Hibi M, Shimizu T
    • Organizer
      13th International Zebrafish Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Optogenetic manipulation of zebrafish neural circuits toward understanding higher order function of the cerebellum.2018

    • Author(s)
      Shimizu T, Matsuda K, Hibi M
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of axonal transport of Reelin in layer and neural circuit formation in the cerebellum and the optic tectum.2018

    • Author(s)
      Nimura T, Di Donata V, Hayashi T, Takeuchi M, Shimizu T, Del Bene F, Hibi M
    • Organizer
      第70回日本細胞生物学会第51回日本発生生物学会合同学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Fat2 controls formation of cerebellar neural circuits in zebrafish.2018

    • Author(s)
      Dohaku R, Takeuchi M, Shimizu T, Hibi M
    • Organizer
      第70回日本細胞生物学会第51回日本発生生物学会合同学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Molecular mechanisms that control development of the inferior olive nucleus neurons in zebrafish.2018

    • Author(s)
      Itoh T, Takeuchi M, Sakagami M, Asakawa K, Kawakami K, Shimizu T, Hibi M
    • Organizer
      第70回日本細胞生物学会第51回日本発生生物学会合同学会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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