• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of new nucleic acid medicines for intractable respiratory diseases

Research Project

Project/Area Number 18H02589
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 47060:Clinical pharmacy-related
Research InstitutionNagasaki University

Principal Investigator

SASAKI Hitoshi  長崎大学, 病院(医学系), 教授 (00170689)

Co-Investigator(Kenkyū-buntansha) 中村 忠博  長崎大学, 病院(医学系), 技術職員 (60882199)
川上 茂  長崎大学, 医歯薬学総合研究科(薬学系), 教授 (20322307)
山下 親正  東京理科大学, 薬学部薬学科, 教授 (30622188)
黒崎 友亮  長崎大学, 医歯薬学総合研究科(薬学系), 助教 (00582016)
室 高広  長崎大学, 病院(医学系), 技術職員 (10832834)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2019: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywords遺伝子デリバリー / 核酸医薬 / ナノ微粒子 / ドラッグデリバリー / ドラッグデリバリーシステム
Outline of Final Research Achievements

In this study, we developed gene and nucleic acid medicines for lung disease based on lung-targeted nanoparticles by systemic or pulmonary administration. Nanoparticles could be constructed by optimizing the mixing ratio of pDNA, cationic compound, and anionic compound for self-assembly. Nanoparticles showed high gene expression in lung selectively after intravenous and pulmonary administration. In addition, the nanoparticles were also able to adapt to siRNA. As a result of adding nanoparticles containing siRNA to luciferase to luciferase expressing mouse melanoma cells, it was shown that they suppressed the luciferase expression. Furthermore, as a result of intravenous administration of nanoparticles to melanoma lung metastasis model mice, lung metastasis was significantly suppressed as compared with the control.

Academic Significance and Societal Importance of the Research Achievements

本研究により、全身投与および経肺投与により選択的に送達できるナノ微粒子の調製に成功した。このナノ微粒子はがん細胞にも良好に取り込まれ、遺伝子発現および抑制をすることができるため、肺がんあるいは肺転移がんに対する遺伝子・核酸医薬開発へ応用することが可能である。また、他の肺疾患にも応用が可能であるため、幅広く医療貢献ができる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (15 results)

All 2021 2020 2019 2018

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (7 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Anionic Complex with Efficient Expression and Good Safety Profile for mRNA Delivery2021

    • Author(s)
      Hamada E, Kurosaki T, Hashizume J, Harasawa H, Nakagawa H, Nakamura T, Kodama Y, Sasaki H.
    • Journal Title

      Pharmaceutics

      Volume: 13 Issue: 1 Pages: 126-126

    • DOI

      10.3390/pharmaceutics13010126

    • NAID

      120006961264

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a DNA Vaccine for Melanoma Metastasis by Inhalation Based on an Analysis of Transgene Expression Characteristics of Naked pDNA and a Ternary Complex in Mouse Lung Tissues2020

    • Author(s)
      Kodama Y, Nakashima M, Nagahara T, Oyama N, Hashizume J, Nakagawa H, Harasawa H, Muro T, Kurosaki T, Yamashita C, Hashida M, Kitahara T, Sasaki H, Kawakami S, Nakamura T
    • Journal Title

      Pharmaceutics

      Volume: 12 Issue: 6 Pages: 540-540

    • DOI

      10.3390/pharmaceutics12060540

    • NAID

      120006988149

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Proliferation‐associated long noncoding RNA, TMPO‐AS1 , is a potential therapeutic target for triple‐negative breast cancer2020

    • Author(s)
      Mitobe Yuichi、Ikeda Kazuhiro、Sato Wataru、Kodama Yukinobu、Naito Mitsuru、Gotoh Noriko、Miyata Kanjiro、Kataoka Kazunori、Sasaki Hitoshi、Horie‐Inoue Kuniko、Inoue Satoshi
    • Journal Title

      Cancer Science

      Volume: 1 Issue: 7 Pages: 1-20

    • DOI

      10.1111/cas.14498

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methotrexate-Coated Complexes of Plasmid DNA and Polyethylenimine for Gene Delivery2018

    • Author(s)
      Kodama Y, Nishigaki W, Nakamura T, Fumoto S, Nishida K, Kurosaki T, Nakagawa H, Kitahara T, Muro T, Sasaki H.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 41 Issue: 10 Pages: 1537-1542

    • DOI

      10.1248/bpb.b18-00144

    • NAID

      130007494219

    • ISSN
      0918-6158, 1347-5215
    • Year and Date
      2018-10-01
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene delivery system of pDNA using the blood glycoprotein fetuin.2018

    • Author(s)
      Kodama Y, Hanamura H, Muro T, Nakagawa H, Kurosaki T, Nakamura T, Kitahara T, Kawakami S, Nakashima M, Sasaki H.
    • Journal Title

      Journal of Drug Targeting

      Volume: 印刷中 Issue: 7 Pages: 604-609

    • DOI

      10.1080/1061186x.2017.1405425

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Splenic Delivery System of pDNA through Complexes Electrostatically Constructed with Protamine and Chondroitin Sulfate2018

    • Author(s)
      Kodama Y, Nishigaki W, Nakamura T, Fumoto S, Nishida K, Kurosaki T, Nakagawa H, Kitahara T, Muro T, Sasaki H.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 41 Issue: 3 Pages: 342-349

    • DOI

      10.1248/bpb.b17-00667

    • NAID

      130006407447

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] RNAワクチン開発を目的としたmRNA内包三元複合体の開発2020

    • Author(s)
      濵田 英里、黑﨑 友亮、兒玉 幸修、室 高広、中村 忠博、佐々木 均
    • Organizer
      日本薬剤学会第35年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 静電的相互作用を基盤としたaptamer被膜型遺伝子ベクターの開発2019

    • Author(s)
      黒崎 友亮、兒玉 幸修、中川 博雄、中村 忠博、大山 要、中嶋 幹郎、川上 茂、佐々木 均
    • Organizer
      日本薬剤学会第34年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 大腸がん腹膜播種に対するMDM2-siRNA複合体の有効性評価2018

    • Author(s)
      丸田 由佳理, 兒玉 幸修, 北原 隆志, 佐々木 均
    • Organizer
      日本薬剤学会第33年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] c-Myc-siRNA三重複合体によるメラノーマ増殖抑制に関する基礎的検討2018

    • Author(s)
      三枝 由香莉, 兒玉 幸修, 北原 隆志, 佐々木 均
    • Organizer
      日本薬剤学会第33年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 大腸がん腹膜播種に対するVEGF-siRNAナノ粒子の開発2018

    • Author(s)
      大倉 真生, 兒玉 幸修, 北原 隆志, 佐々木 均
    • Organizer
      第34回日本DDS学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 生体分解型siRNAベクターの構築と有用性評価2018

    • Author(s)
      柿木 優希, 兒玉 幸修, 北原 隆志, 佐々木 均
    • Organizer
      第34回日本DDS学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] VEGF-siRNA複合体によるメラノーマ肺転移の抑制2018

    • Author(s)
      岡田 明莉, 兒玉 幸修, 北原 隆志, 佐々木 均
    • Organizer
      第34回日本DDS学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] 肺指向性薬物送達体2019

    • Inventor(s)
      佐々木均、兒玉幸修、川上茂
    • Industrial Property Rights Holder
      佐々木均、兒玉幸修、川上茂
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-098677
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 経肺投与用薬物送達体2019

    • Inventor(s)
      佐々木均、兒玉幸修、川上茂
    • Industrial Property Rights Holder
      佐々木均、兒玉幸修、川上茂
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-099112
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi