Development of new nucleic acid medicines for intractable respiratory diseases
| Project/Area Number |
18H02589
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 47060:Clinical pharmacy-related
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| Research Institution | Nagasaki University |
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Principal Investigator |
SASAKI Hitoshi 長崎大学, 病院(医学系), 教授 (00170689)
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| Co-Investigator(Kenkyū-buntansha) |
中村 忠博 長崎大学, 病院(医学系), 技術職員 (60882199)
川上 茂 長崎大学, 医歯薬学総合研究科(薬学系), 教授 (20322307)
山下 親正 東京理科大学, 薬学部薬学科, 教授 (30622188)
黒崎 友亮 長崎大学, 医歯薬学総合研究科(薬学系), 助教 (00582016)
室 高広 長崎大学, 病院(医学系), 技術職員 (10832834)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2019: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Keywords | 遺伝子デリバリー / 核酸医薬 / ナノ微粒子 / ドラッグデリバリー / ドラッグデリバリーシステム |
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| Outline of Final Research Achievements |
In this study, we developed gene and nucleic acid medicines for lung disease based on lung-targeted nanoparticles by systemic or pulmonary administration. Nanoparticles could be constructed by optimizing the mixing ratio of pDNA, cationic compound, and anionic compound for self-assembly. Nanoparticles showed high gene expression in lung selectively after intravenous and pulmonary administration. In addition, the nanoparticles were also able to adapt to siRNA. As a result of adding nanoparticles containing siRNA to luciferase to luciferase expressing mouse melanoma cells, it was shown that they suppressed the luciferase expression. Furthermore, as a result of intravenous administration of nanoparticles to melanoma lung metastasis model mice, lung metastasis was significantly suppressed as compared with the control.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、全身投与および経肺投与により選択的に送達できるナノ微粒子の調製に成功した。このナノ微粒子はがん細胞にも良好に取り込まれ、遺伝子発現および抑制をすることができるため、肺がんあるいは肺転移がんに対する遺伝子・核酸医薬開発へ応用することが可能である。また、他の肺疾患にも応用が可能であるため、幅広く医療貢献ができる。
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Development of a DNA Vaccine for Melanoma Metastasis by Inhalation Based on an Analysis of Transgene Expression Characteristics of Naked pDNA and a Ternary Complex in Mouse Lung Tissues2020
Author(s)
Kodama Y, Nakashima M, Nagahara T, Oyama N, Hashizume J, Nakagawa H, Harasawa H, Muro T, Kurosaki T, Yamashita C, Hashida M, Kitahara T, Sasaki H, Kawakami S, Nakamura T
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Journal Title
Pharmaceutics
Volume: 12
Issue: 6
Pages: 540-540
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Methotrexate-Coated Complexes of Plasmid DNA and Polyethylenimine for Gene Delivery2018
Author(s)
Kodama Y, Nishigaki W, Nakamura T, Fumoto S, Nishida K, Kurosaki T, Nakagawa H, Kitahara T, Muro T, Sasaki H.
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 41
Issue: 10
Pages: 1537-1542
DOI
NAID
ISSN
0918-6158, 1347-5215
Year and Date
2018-10-01
Related Report
Peer Reviewed
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[Journal Article] Splenic Delivery System of pDNA through Complexes Electrostatically Constructed with Protamine and Chondroitin Sulfate2018
Author(s)
Kodama Y, Nishigaki W, Nakamura T, Fumoto S, Nishida K, Kurosaki T, Nakagawa H, Kitahara T, Muro T, Sasaki H.
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 41
Issue: 3
Pages: 342-349
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed
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