Effect on virulence of S. suis by switching the surface antigenicity

• Project/Area Number: 18H02658
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 49050:Bacteriology-related
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Okura Masatoshi 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門, 上級研究員 (60508315)
• Co-Investigator(Kenkyū-buntansha): 芝原 友幸 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門, グループ長補佐 (00355207)
• Project Period (FY): 2018-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000); Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
• Keywords: 細菌 / 表層抗原 / 血清型 / 抗原変換 / 細菌学 / 人獣共通感染症 / 人獣共通感染症起因菌 / レンサ球菌 / 表層抗原変換 / 抗原性変換 / Streptococcus suis / 莢膜型 / リポタイコ酸

Outline of Final Research Achievements

In the present study, several types of surface antigen switching mutants of Streptococcus suis were constructed with the method developed by the authors, and then compared with parent strain on virulence in mice and piglets, colonization on/invasion in the host cells, resistance against macrophage phagocytosis and the bactericidal effect of leukocytes, etc. The results provides the first evidence that serotype switch in S. suis can definitively modify the interactions with host cells and in vivo.

Academic Significance and Societal Importance of the Research Achievements

本研究成果により、血清型の相違が動物への病原性や宿主細胞との相互作用に多様に影響することを実証した。血清型の相違による表現型への影響については、多くの病原細菌において、よく分かっておらず、血清型によっては、病原性が高くなることが示唆される型も明らかにした。本菌については、有効なワクチンがまだ開発されておらず、表層抗原はワクチンの開発にもつながるため、得られた成果を活用することにより、本菌の有効な新規ワクチンも期待できる。以上から、本研究成果は学術的にも社会的にも非常に重要な成果と見なすことができる。

Research Products

• [Int'l Joint Research] モントリオール大学獣医学部(カナダ)
• [Int'l Joint Research] モントリオール大学(カナダ)
• [Int'l Joint Research] ボルステル研究センター(ドイツ)
• [Journal Article] Update on Streptococcus suis Research and Prevention in the Era of Antimicrobial Restriction: 4th International Workshop on S. suis (2020)
  • Author(s): Mariela Segura, Virginia Aragon, Susan L Brockmeier, Connie Gebhart, Astrid de Greeff, Anusak Kerdsin, Mark A O'Dea, Masatoshi Okura, Mariette Salery, Constance Schultsz, Peter Valentin-Weigand, Lucy A Weinert, Jerry M Wells, Marcelo Gottschalk
  • Journal Title: Pathogens Volume: 9 Issue: 5 Pages: 374-374
  • DOI: 10.3390/pathogens9050374
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of a mismatch amplification mutation assay to correctly serotype isolates of Streptococcus suis serotypes 1, 2, 1/2, and 14 (2020)
  • Author(s): Sonia Lacouture, Masatoshi Okura, Daisuke Takamatsu, Lorelei Corsaut, Marcelo Gottschalk
  • Journal Title: Journal of Veterinary Diagnostic Investigation Volume: 32 Issue: 3 Pages: 490-494
  • DOI: 10.1177/1040638720915869
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Structure determination of Streptococcus suis serotypes 7 and 8 capsular polysaccharides and assignment of functions of the cps locus genes involved in their biosynthesis (2019)
  • Author(s): Goyette-Desjardins Guillaume、Vinogradov Evgeny、Okura Masatoshi、Takamatsu Daisuke、Gottschalk Marcelo、Segura Mariela
  • Journal Title: Carbohydrate Research Volume: 473 Pages: 36-45
  • DOI: 10.1016/j.carres.2018.12.009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Streptococcus suis serotype 3 and serotype 18 capsular polysaccharides contain di- N -acetyl-bacillosamine (2018)
  • Author(s): Goyette-Desjardins Guillaume、Vinogradov Evgeny、Okura Masatoshi、Takamatsu Daisuke、Gottschalk Marcelo、Segura Mariela
  • Journal Title: Carbohydrate Research Volume: 466 Pages: 18-29
  • DOI: 10.1016/j.carres.2018.07.003
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Capsular Sialyltransferase Specificity Mediates Different Phenotypes in Streptococcus suis and Group B Streptococcus (2018)
  • Author(s): Roy David、Takamatsu Daisuke、Okura Masatoshi、Goyette-Desjardins Guillaume、Van Calsteren Marie-Rose、Dumesnil Audrey、Gottschalk Marcelo、Segura Mariela
  • Journal Title: Frontiers in Microbiology Volume: 9 Pages: 545-545
  • DOI: 10.3389/fmicb.2018.00545
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Serotype switching can modulate virulence in Streptococcus suis (2023)
  • Author(s): M. Okura、J-P Auger、T. Shibahara、G. Goyette-Desjardins、M-R Van Calsteren、 F. Maruyama、M. Kawai、M. Segura、M. Gottschalk、 D. Takamatsu
  • Organizer: 第96回日本細菌学会総会
• [Presentation] Switching of capsular type impacts on Streptococcus suis virulence in mice and pigs (2019)
  • Author(s): Masatoshi Okura, Jean-Philippe Auger, Tomoyuki Shibahara, Guillaume Goyette-Desjardins, Marie-Rose Van Calsteren, Fumito Maruyama, Makoto Osaki, Mariela Segura, Marcelo Gottschalk, Daisuke Takamatsu
  • Organizer: 4th international workshop on Streptococcus suis
  • Int'l Joint Research / Invited