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Analysis of the mechanisms by which intra-tumor metabolic microenvironment leads to radioresistance

Research Project

Project/Area Number 18H02759
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 52040:Radiological sciences-related
Research InstitutionHokkaido University

Principal Investigator

Onodera Yasuhito  北海道大学, 医学研究院, 准教授 (90435561)

Co-Investigator(Kenkyū-buntansha) 及川 司  北海道大学, 医学研究院, 講師 (20457055)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Keywords放射線 / 代謝協調 / 細胞間相互作用 / 糖代謝 / 共培養 / 代謝 / 放射線耐性 / 治療耐性
Outline of Final Research Achievements

Using cell-specific regulation of glucose metabolism, we co-cultured glucose-sufficient and glucose-deficient cancer cells and established "metabolic cooperation" between them to analyze viability, intracellular metabolism, phenotypic changes, and response to radiation. The above experimental system revealed that cancer cells in a glucose-depleted state maintain their viability by obtaining metabolites from glucose-sufficient cells, and that oxidative stress and ER stress in the former cells are markedly suppressed. Estimation of the mediating substances and mechanisms, as well as the various phenotypic changes that occur in the metabolic cooperative state, are currently under analysis.

Academic Significance and Societal Importance of the Research Achievements

腫瘍の内部はがん細胞の盛んな増殖と代謝変化とが相まって、しばしば栄養素や酸素の欠乏領域を生じる。そのような領域では薬剤や放射線の効果、および免疫細胞の作用が抑制されるため、治療効率が低下する。また、申請者自身の解析により、栄養素欠乏領域の存在は、周囲のがん細胞も含めた腫瘍全体の治療耐性亢進を誘導することが明らかとなった。栄養欠乏領域のがん細胞は、周囲の細胞の中間代謝産物を利用して生存性を維持している。このような「代謝協調」のメカニズムを明らかにし、それに基づき飢餓領域において細胞死を効率よく誘導する方法を確立できれば、既存の治療法の効率をより高めることができると考えられる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (4 results)

All 2020 2019

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 4 results)

  • [Journal Article] Lysosomal trafficking mediated by Arl8b and BORC promotes invasion of cancer cells that survive radiation.2020

    • Author(s)
      Wu PH, Onodera Y, Giaccia AJ, Le QT, Shimizu S, Shirato H, Nam JM.
    • Journal Title

      Communications Biology

      Volume: 27 Issue: 1 Pages: 1-15

    • DOI

      10.1038/s42003-020-01339-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Rab27b contributes to radioresistance and exerts a paracrine effect via epiregulin in glioblastoma.2020

    • Author(s)
      Nishioka S, Wu PH, Yakabe T, Giaccia AJ, Le QT, Aoyama H, Shimizu S, Shirato H, Onodera Y, Nam JM.
    • Journal Title

      Neuro-Oncology Advances

      Volume: 8 Issue: 1 Pages: 1-13

    • DOI

      10.1093/noajnl/vdaa091

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lambda-Carrageenan Enhances the Effects of Radiation Therapy in Cancer Treatment by Suppressing Cancer Cell Invasion and Metastasis through Racgap1 Inhibition2019

    • Author(s)
      Wu Ping-Hsiu、Onodera Yasuhito、Recuenco Frances C.、Giaccia Amato J.、Le Quynh-Thu、Shimizu Shinichi、Shirato Hiroki、Nam Jin-Min
    • Journal Title

      Cancers

      Volume: 11 Issue: 8 Pages: 1192-1192

    • DOI

      10.3390/cancers11081192

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Targeting Integrins in Cancer Nanomedicine: Applications in Cancer Diagnosis and Therapy2019

    • Author(s)
      Wu Ping-Hsiu、Opadele Abayomi Emmanuel、Onodera Yasuhito、Nam Jin-Min
    • Journal Title

      Cancers

      Volume: 11 Issue: 11 Pages: 1783-1783

    • DOI

      10.3390/cancers11111783

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access

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Published: 2018-04-23   Modified: 2022-01-27  

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