Development of a method of functional integration between host and graft on the transplantation therapy of human iPS cell-derived cardiac tissues
| Project/Area Number |
18H02887
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 55030:Cardiovascular surgery-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
湊谷 謙司 京都大学, 医学研究科, 教授 (20393241)
池田 義 京都大学, 医学研究科, 准教授 (40281092)
柴 祐司 信州大学, 学術研究院医学系, 教授 (70613503)
升本 英利 国立研究開発法人理化学研究所, 生命機能科学研究センター, 上級研究員 (70645754)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | 心臓再生医療 / iPS細胞 / バイオエンジニアリング / 再生医学 |
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| Outline of Final Research Achievements |
In this study, we found very low propagation speed of iPS cell-derived cardiac tissues due to low expression level of KCNJ2 gene coding IK1 channel. To solve this problem, we conducted a combination of 3-dimensional composition of iPS cell-derived cardiomyocytes and vascular cells, and integration of electrical stimulation and dynamic rocking culture training and confirmed structural and electrophysiological maturation of the tissues. We also established a system to evaluate graft-to-host integration and local wall motion using trans-esophageal echocardiogram in a pig myocardial infarction model.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、これまでのiPS細胞による再生医療における治療メカニズムであるパラクライン効果を超えた、動力源としての心筋組織を疾患心に供給し、かつ機能させるという次世代の心臓再生医療の実現に向けた基礎研究的基盤を供給するものである。今後ますます増加が予想される重症心不全に対する新たな治療選択肢を供給しうる意味において、高い学術的ならびに社会的意義を有するものと考えられる。
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Report
(4 results)
Research Products
(63 results)
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[Journal Article] Chronic optical pacing conditioning of h-iPSC engineered cardiac tissues.2019
Author(s)
Dwenger M, Kowalski WJ, Ye F, Yuan F, Tinney JP, Setozaki S, Nakane T, Masumoto H, Campbell P, Guido W, Keller BB.
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Journal Title
J Tissue Eng
Volume: 10
Pages: 204173141984174-204173141984174
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A therapeutic angiogenesis of sustained release of basic fibroblast growth factor using biodegradable gelatin hydrogel sheets in a canine chronic myocardial infarction model.2018
Author(s)
Kumagai M, Minakata K, Masumoto H, Yamamoto M, Yonezawa A, Ikeda T, Uehara K, Yamazaki K, Ikeda T, Matsubara K, Yokode M, Shimizu A, Tabata Y, Sakata R, Minatoya K.
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Journal Title
Heart Vessels
Volume: 33
Issue: 10
Pages: 1251-1257
DOI
Related Report
Peer Reviewed
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[Presentation] Surgical management for heart failure:A comparison of left ventricular repair and ventricular assist device2019
Author(s)
Kanemitsu H, Minatoya K, Yamazaki K, Sakamoto K, Kawatou M, Sakai J, Kudo M, Nakamura M, Yamamoto R, Kitagata Y, Ide Y, Ikeda T
Organizer
ISHLT2019
Related Report
Int'l Joint Research
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