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Molecular dissection of peptide signaling in plants

Research Project

Project/Area Number 18H05274
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Review Section Broad Section G
Research InstitutionNagoya University

Principal Investigator

MATSUBAYASHI Yoshikatsu  名古屋大学, 理学研究科, 教授 (00313974)

Project Period (FY) 2018-06-11 – 2023-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥192,530,000 (Direct Cost: ¥148,100,000、Indirect Cost: ¥44,430,000)
Fiscal Year 2022: ¥39,910,000 (Direct Cost: ¥30,700,000、Indirect Cost: ¥9,210,000)
Fiscal Year 2021: ¥39,910,000 (Direct Cost: ¥30,700,000、Indirect Cost: ¥9,210,000)
Fiscal Year 2020: ¥39,910,000 (Direct Cost: ¥30,700,000、Indirect Cost: ¥9,210,000)
Fiscal Year 2019: ¥39,910,000 (Direct Cost: ¥30,700,000、Indirect Cost: ¥9,210,000)
Fiscal Year 2018: ¥32,890,000 (Direct Cost: ¥25,300,000、Indirect Cost: ¥7,590,000)
Keywordsペプチドホルモン / 受容体キナーゼ / 窒素栄養 / シロイヌナズナ / ストレス応答 / 長距離移行シグナル / 受容体 / 形態形成 / 環境応答
Outline of Final Research Achievements

The following results were obtained by a new approach that focuses on molecules and traces them back to mechanisms. (i) the discovery of a novel peptide hormone PSY and its receptor PSYR that controls stress response and growth switching in plant cells; (ii) the discovery of the phloem-mobile peptide signal CEPDL2, which communicates leaf nitrogen demand to the roots; (iii) the discovery of the protein phosphatase CEPH, which acts downstream of CEPDL2 and activates the nitrate transporter NRT2.1; and (iv) discovery of proteases that cleave peptide ligands that induce immune response from flagellin, the major protein constituting the bacterial flagellum.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は,動物のように動き回ることはできない植物が,様々な環境変化に対してどのように適応しているかの一端を解き明かしたものである.ペプチドホルモンPSYの発見は,トレードオフの関係にあるストレス応答と成長能力のバランスの人為的制御につながるブレイクスルーであり,CEPDL2やCEPHの発見は,植物の窒素栄養吸収のしくみの理解を飛躍的に深めるものである.フラジェリンから植物免疫を誘導するペプチドリガンドを切り出すプロテアーゼの発見は,植物が病原細菌を認識するしくみを理解する上での重要な手掛かりとなる.いずれの知見も農作物の生産性向上に応用可能である.

Assessment Rating
Ex-post Assessment Comments (Rating)

A: In light of the aim of introducing the research area into the research categories, expected outcomes of research have been produced.

Assessment Rating
Interim Assessment Comments (Rating)

A: In light of the aim of introducing the research area into the research categories, the expected progress has been made in research.

Report

(11 results)
  • 2024 Ex-post Assessment (Comments) ( PDF )
  • 2023 Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Abstract (Interim Assessment) ( PDF )   Annual Research Report   Interim Assessment (Comments) ( PDF )
  • 2019 Annual Research Report
  • 2018 Abstract ( PDF )   Comments on the Screening Results ( PDF )   Annual Research Report
  • Research Products

    (32 results)

All 2024 2023 2022 2021 2020 2019 2018 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 7 results) Presentation (15 results) (of which Int'l Joint Research: 14 results,  Invited: 15 results) Book (3 results) Remarks (5 results)

  • [Journal Article] Arabidopsis SBT5.2 and SBT1.7 subtilases mediate C-terminal cleavage of flg22 epitope from bacterial flagellin2024

    • Author(s)
      Matsui Sayaka、Noda Saki、Kuwata Keiko、Nomoto Mika、Tada Yasuomi、Shinohara Hidefumi、Matsubayashi Yoshikatsu
    • Journal Title

      Nature Communications

      Volume: 15 Issue: 1

    • DOI

      10.1038/s41467-024-48108-4

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Peptide ligand-mediated trade-off between plant growth and stress response2022

    • Author(s)
      Mari Ogawa-Ohnishi, Tomohide Yamashita, Mitsuru Kakita, Takuya Nakayama, Yuri Ohkubo, Yoko Hayashi, Yasuko Yamashita, Taizo Nomura, Saki Noda, Hidefumi Shinohara, Yoshikatsu Matsubayashi
    • Journal Title

      Science

      Volume: 378 Issue: 6616 Pages: 175-180

    • DOI

      10.1126/science.abq5735

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A type 2C protein phosphatase activates high-affinity nitrate uptake by dephosphorylating NRT2.12021

    • Author(s)
      Ohkubo Yuri、Kuwata Keiko、Matsubayashi Yoshikatsu
    • Journal Title

      Nature Plants

      Volume: 7 Issue: 3 Pages: 310-316

    • DOI

      10.1038/s41477-021-00870-9

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Shoot-to-root mobile CEPD-like 2 integrates shoot nitrogen status to systemically regulate nitrate uptake in Arabidopsis2020

    • Author(s)
      Ota Ryosuke、Ohkubo Yuri、Yamashita Yasuko、Ogawa-Ohnishi Mari、Matsubayashi Yoshikatsu
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 641-641

    • DOI

      10.1038/s41467-020-14440-8

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Screening and identification of a non-peptide antagonist for the peptide hormone receptor in Arabidopsis.2019

    • Author(s)
      Shinohara, H., Yasue, N., Onuki, T., Yoshida, M. Matsubayashi, Y.
    • Journal Title

      Commun. Biol. Commun Biol.

      Volume: 2 Issue: 1 Pages: 61-61

    • DOI

      10.1038/s42003-019-0307-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lateral inhibition by a peptide hormone-receptor cascade during Arabidopsis lateral root founder cell formation.2019

    • Author(s)
      Toyokura K, Goh T, Shinohara H, Shinoda A, Kondo Y, Okamoto Y, Uehara T, Fujimoto K, Okushima Y, Ikeyama Y, Nakajima K, Mimura T, Tasaka M, Matsubayashi Y and Fukaki H.
    • Journal Title

      Developmental Cell

      Volume: 48 Issue: 1 Pages: 64-75

    • DOI

      10.1016/j.devcel.2018.11.031

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pectin RG-I rhamnosyltransferases represent a novel plant-specific glycosyltransferase family2018

    • Author(s)
      Takenaka Yuto、Kato Kohei、Ogawa-Ohnishi Mari、Tsuruhama Kana、Kajiura Hiroyuki、Yagyu Kenta、Takeda Atsushi、Takeda Yoichi、Kunieda Tadashi、Hara-Nishimura Ikuko、Kuroha Takeshi、Nishitani Kazuhiko、Matsubayashi Yoshikatsu、Ishimizu Takeshi
    • Journal Title

      Nature Plants

      Volume: 4 Issue: 9 Pages: 669-676

    • DOI

      10.1038/s41477-018-0217-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] PLENTY, a hydroxyproline O-arabinosyltransferase, negatively regulates root nodule symbiosis in Lotus japonicus2018

    • Author(s)
      Yoro Emiko、Nishida Hanna、Ogawa-Ohnishi Mari、Yoshida Chie、Suzaki Takuya、Matsubayashi Yoshikatsu、Kawaguchi Masayoshi
    • Journal Title

      Journal of Experimental Botany

      Volume: 70 Issue: 2 Pages: 507-517

    • DOI

      10.1093/jxb/ery364

    • NAID

      120007128131

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Exploring peptide hormones in plants: identification of four peptide hormone-receptor pairs and two post-translational modification enzymes2018

    • Author(s)
      MATSUBAYASHI Yoshikatsu
    • Journal Title

      Proceedings of the Japan Academy, Series B

      Volume: 94 Issue: 2 Pages: 59-74

    • DOI

      10.2183/pjab.94.006

    • NAID

      130006339623

    • ISSN
      0386-2208, 1349-2896
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Peptide signal-mediated adaptation to spatially and temporally flucuating environments in plants2023

    • Author(s)
      松林 嘉克
    • Organizer
      The 33rd International Conference on Arabidopsis Research
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Plant adaptation to fluctuating nitrogen environments by long-range mobile peptide signals2023

    • Author(s)
      松林 嘉克
    • Organizer
      11th Plant Peptides and Receptors Meeting
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Plant adaptation to fluctuating nitrogen environments by long-range mobile peptide signals2023

    • Author(s)
      松林 嘉克
    • Organizer
      Nitrogen2023
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Exploring peptide hormones in plants: Identification of five peptide hormone-receptor pairs and two post-translational modification enzymes2023

    • Author(s)
      松林 嘉克
    • Organizer
      The 24th International Conference on Plant Growth Substances
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 植物の窒素吸収を制御する長距離移行ペプチド群2021

    • Author(s)
      大久保祐里,松林嘉克
    • Organizer
      第44回日本分子生物学会年会ワークショップ 『不均一環境変動に対する植物の情報統御機構』
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Receptor kinase signaling in plants: from peptide ligand identification to non-peptide antagonist development.2019

    • Author(s)
      Matsubayashi Y.
    • Organizer
      International Symposium on Plant Receptor Kinases and Cell Signaling 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long-distance peptide signaling involved in systemic regulation of nitrogen acquisition.2019

    • Author(s)
      Matsubayashi Y.
    • Organizer
      The International Plant Growth Substances Association (IPGSA) Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long distance peptide signaling mediating nitrogen homeostasis.2019

    • Author(s)
      Matsubayashi Y.
    • Organizer
      International Workshop on Plant Membrane Biology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long distance peptide signaling mediating nitrogen homeostasis.2019

    • Author(s)
      Matsubayashi Y.
    • Organizer
      The 4th International Symposium on the Nitrogen Nutrition of Plants
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long distance peptide signaling mediating nitrogen homeostasis.2019

    • Author(s)
      Matsubayashi Y
    • Organizer
      Frontiers in plant environmental response research
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long distance peptide signaling mediating nitrogen homeostasis.2019

    • Author(s)
      Matsubayashi Y
    • Organizer
      Cold Spring Harbor Asia conference on Plant Cell and Development Biology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long-distance peptide signaling involved in systemic regulation of nitrogen acquisition2018

    • Author(s)
      Matsubayashi Yoshikatsu
    • Organizer
      Sainsbury Laboratory Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Long-distance peptide signaling involved in systemic regulation of nitrogen acquisition2018

    • Author(s)
      Matsubayashi Yoshikatsu
    • Organizer
      Frontiers in BioAgricultural Research
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Root-to-shoot and shoot-to-root long-distance mobile peptides mediate systemic regulation of nitrogen acquisition2018

    • Author(s)
      Matsubayashi Yoshikatsu
    • Organizer
      SEB's Annual Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Root-to-shoot and shoot-to-root long-distance mobile peptides mediate systemic regulation of nitrogen acquisition2018

    • Author(s)
      Matsubayashi Yoshikatsu
    • Organizer
      Keystone Symposia, Plant Signaling: Molecular Pathways and Network Integration
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] 窒素取り込みを制御する長距離移行ペプチド群2021

    • Author(s)
      大久保祐里,松林嘉克
    • Publisher
      アグリバイオ/北隆館
    • Related Report
      2021 Annual Research Report
  • [Book] 植物の窒素栄養吸収制御の巧みなしくみ2021

    • Author(s)
      大久保祐里,松林嘉克
    • Publisher
      生化学/日本生化学会
    • Related Report
      2021 Annual Research Report
  • [Book] 生物の科学 遺伝2020

    • Author(s)
      大久保 祐里,松林 嘉克
    • Total Pages
      160
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2020 Annual Research Report
  • [Remarks] 細胞間シグナル研究グループ

    • URL

      https://www.bio.nagoya-u.ac.jp/~b2/

    • Related Report
      2022 Annual Research Report
  • [Remarks] 細胞間シグナル研究グループHP

    • URL

      https://www.bio.nagoya-u.ac.jp/~b2/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 名古屋大学大学院理学研究科生命理学専攻 細胞間シグナル研究グループ

    • URL

      https://www.bio.nagoya-u.ac.jp/~b2/index.html

    • Related Report
      2020 Annual Research Report
  • [Remarks] 名古屋大学 大学院理学研究科 生命理学専攻 細胞間シグナル研究グループ(松林嘉克教授)ホームページ

    • URL

      https://www.bio.nagoya-u.ac.jp/~b2/index.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 名古屋大学 大学院理学研究科 生命理学専攻 細胞間シグナル研究グループ(松林嘉克教授)ホームページ

    • URL

      http://www.bio.nagoya-u.ac.jp/~b2/index.html

    • Related Report
      2018 Annual Research Report

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Published: 2018-06-12   Modified: 2025-02-04  

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