• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

幹細胞および薬剤の関節内投与による重度変形性関節症の病態改善治療

Research Project

Project/Area Number 18J01264
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Rehabilitation science/Welfare engineering
Research InstitutionThe University of Tokyo

Principal Investigator

千々松 良太  東京大学, 医学系研究科, 特任助教 (60803210)

Project Period (FY) 2018-04-25 – 2021-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords変形性関節症 / 間葉系幹細胞 / 行動解析 / 疼痛 / 骨棘
Outline of Annual Research Achievements

変形性膝関節症を治療し、その指標を痛みや運動能とするという目的において、報告のあるマウスのOAモデル(外科モデル2つ、コラゲナーゼモデル、パパインモデル、MIAモデル)を全て検証し、それぞれのOAモデルに特徴的な症状(軟骨の変性、骨病変、炎症など)を把握することができた。これらのモデルでの疼痛を評価するため、市販の行動解析を行ったが、膝の損傷がマウスの痛み行動として検出されないことが明らかになった。末梢神経(関節内、関節周囲)、後根神経節、脊髄レベルでの評価もそれに相当してマウスの膝痛を捉えることはできなかった。マウスを用いたOA解析では、痛み以外に、炎症、骨棘形成を見るツールとしての有用性を感じ、炎症のin vivo imagingや骨棘発生のμCT解析の系を立ち上げた。
次に幹細胞に関しては、従来難しいとされていたマウスの脂肪幹細胞、滑膜幹細胞の安定した培養法を確立し、動物へ投与可能な量を調製することが可能となった。ヒトの膝手術時
の余剰検体からも幹細胞を採取し、各組織由来幹細胞の異なる表現型について詳細に解析した。これらの検証から、幹細胞の性能をin vitroで評価する系を考案し、汎用性のあるアッセイ樹立に着手している。

Research Progress Status

翌年度、交付申請を辞退するため、記入しない。

Strategy for Future Research Activity

翌年度、交付申請を辞退するため、記入しない。

Report

(2 results)
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (31 results)

All 2020 2019 2018

All Journal Article (14 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 12 results,  Open Access: 8 results) Presentation (17 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Primary culture of mouse adipose and fibrous synovial fibroblasts under normoxic and hypoxic conditions2020

    • Author(s)
      SUGIMOTO Hikaru、MURAHASHI Yasutaka、CHIJIMATSU Ryota、MIWA Satoshi、YANO Fumiko、TANAKA Sakae、SAITO Taku
    • Journal Title

      Biomedical Research

      Volume: 41 Issue: 1 Pages: 43-51

    • DOI

      10.2220/biomedres.41.43

    • NAID

      130007801491

    • ISSN
      0388-6107, 1880-313X
    • Year and Date
      2020-02-01
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Associations of clinical outcomes and MRI findings in intra-articular administration of autologous adipose-derived stem cells for knee osteoarthritis2020

    • Author(s)
      Higuchi Junya、Yamagami Ryota、Matsumoto Takumi、Terao Tomohiro、Inoue Keita、Tsuji Shinsaku、Maenohara Yuji、Matsuzaki Tokio、Chijimatsu Ryota、Omata Yasunori、Yano Fumiko、Tanaka Sakae、Saito Taku
    • Journal Title

      Regenerative Therapy

      Volume: 14 Pages: 332-340

    • DOI

      10.1016/j.reth.2020.04.003

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adipose Tissue-Derived Stem Cells Have the Ability to Differentiate into Alveolar Epithelial Cells and Ameliorate Lung Injury Caused by Elastase-Induced Emphysema in Mice.2019

    • Author(s)
      Fukui E, Funaki S, Kimura K, Momozane T, Kimura A, Chijimatsu R, Kanzaki R, Kanou T, Ose N, Minami M, Miyagawa S, Sawa Y, Okumura M, Shintani Y.
    • Journal Title

      Stem Cells Int.

      Volume: 2019 Pages: 5179172-5179172

    • DOI

      10.1155/2019/5179172

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adamts17 is involved in skeletogenesis through modulation of BMP-Smad1/5/8 pathway2019

    • Author(s)
      Oichi Takeshi、Taniguchi Yuki、Soma Kazuhito、Oshima Yasushi、Yano Fumiko、Mori Yoshifumi、Chijimatsu Ryota、Kim-Kaneyama Joo-ri、Tanaka Sakae、Saito Taku
    • Journal Title

      Cellular and Molecular Life Sciences

      Volume: 76 Issue: 23 Pages: 4795-4809

    • DOI

      10.1007/s00018-019-03188-0

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mechanisms of synovial joint and articular cartilage development2019

    • Author(s)
      Chijimatsu Ryota、Saito Taku
    • Journal Title

      Cellular and Molecular Life Sciences

      Volume: 76 Issue: 20 Pages: 3939-3952

    • DOI

      10.1007/s00018-019-03191-5

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Wnt/β-catenin signaling contributes to articular cartilage homeostasis through lubricin induction in the superficial zone2019

    • Author(s)
      Xuan Fengjun、Yano Fumiko、Mori Daisuke、Chijimatsu Ryota、Maenohara Yuji、Nakamoto Hideki、Mori Yoshifumi、Makii Yuma、Oichi Takeshi、Taketo Makoto Mark、Hojo Hironori、Ohba Shinsuke、Chung Ung-il、Tanaka Sakae、Saito Taku
    • Journal Title

      Arthritis Research & Therapy

      Volume: 21 Issue: 1 Pages: 247-247

    • DOI

      10.1186/s13075-019-2041-5

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Oral administration of EP4-selective agonist KAG-308 suppresses mouse knee osteoarthritis development through reduction of chondrocyte hypertrophy and TNF secretion2019

    • Author(s)
      Murahashi Yasutaka、Yano Fumiko、Chijimatsu Ryota、Nakamoto Hideki、Maenohara Yuji、Amakawa Masahiro、Miyake Yoshihide、Yamanaka Hiroyuki、Iba Kousuke、Yamashita Toshihiko、Tanaka Sakae、Saito Taku
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 20329-20329

    • DOI

      10.1038/s41598-019-56861-6

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 関節の発生のメカニズムと臨床応用を目指した今後の展望2019

    • Author(s)
      千々松良太、齋藤琢
    • Journal Title

      THE BONE

      Volume: 33 Pages: 79-86

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Effect of the small compound TD ‐198946 on glycosaminoglycan synthesis and transforming growth factor β3‐associated chondrogenesis of human synovium‐derived stem cells in vitro2019

    • Author(s)
      Chijimatsu Ryota、Yano Fumiko、Saito Taku、Kobayashi Masato、Hamamoto Shuichi、Kaito Takashi、Kushioka Junichi、Hart David A.、Chung Ung‐il、Tanaka Sakae、Yoshikawa Hideki、Nakamura Norimasa
    • Journal Title

      Journal of Tissue Engineering and Regenerative Medicine

      Volume: 13 Issue: 3 Pages: 446-458

    • DOI

      10.1002/term.2795

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Intervertebral disc regeneration with an adipose mesenchymal stem cell-derived tissue-engineered construct in a rat nucleotomy model2019

    • Author(s)
      Ishiguro Hiroyuki、Kaito Takashi、Yarimitsu Seido、Hashimoto Kunihiko、Okada Rintaro、Kushioka Junichi、Chijimatsu Ryota、Takenaka Shota、Makino Takahiro、Sakai Yusuke、Moriguchi Yu、Otsuru Satoru、Hart David A.、Fujie Hiromichi、Nakamura Norimasa、Yoshikawa Hideki
    • Journal Title

      Acta Biomaterialia

      Volume: 87 Pages: 118-129

    • DOI

      10.1016/j.actbio.2019.01.050

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A novel and efficient method for culturing mouse nucleus pulposus cells2019

    • Author(s)
      Kushioka Junichi、Kaito Takashi、Chijimatsu Ryota、Okada Rintaro、Ishiguro Hiroyuki、Bal Zeynep、Kodama Joe、Takenaka Shota、Makino Takahiro、Sakai Yusuke、Yoshikawa Hideki
    • Journal Title

      The Spine Journal

      Volume: epub Issue: 9 Pages: 1573-1583

    • DOI

      10.1016/j.spinee.2019.04.005

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 特集 間葉系幹細胞治療の現状と課題 間葉系幹細胞を用いた再生医療の現状と課題2018

    • Author(s)
      千々松 良太、中村 憲正
    • Journal Title

      整形・災害外科

      Volume: 61 Issue: 11 Pages: 1329-1336

    • DOI

      10.18888/se.0000000635

    • ISSN
      0387-4095
    • Year and Date
      2018-10-01
    • Related Report
      2018 Annual Research Report
  • [Journal Article] Impact of dexamethasone concentration on cartilage tissue formation from human synovial derived stem cells in vitro.2018

    • Author(s)
      Chijimatsu R, Kobayashi M, Ebina K, Iwahashi T, Okuno Y, Hirao M, Fukuhara A, Nakamura N, Yoshikawa H.
    • Journal Title

      Cytotechnology

      Volume: 70 Issue: 2 Pages: 819-829

    • DOI

      10.1007/s10616-018-0191-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] First-in-Human Pilot Study of Implantation of a Scaffold-Free Tissue-Engineered Construct Generated From Autologous Synovial Mesenchymal Stem Cells for Repair of Knee Chondral Lesions2018

    • Author(s)
      Shimomura Kazunori、Yasui Yukihiko、Koizumi Kota、Chijimatsu Ryota、Hart David A.、Yonetani Yasukazu、Ando Wataru、Nishii Takashi、Kanamoto Takashi、Horibe Shuji、Yoshikawa Hideki、Nakamura Norimasa、Sakaue Morito、Sugita Norihiko、Moriguchi Yu
    • Journal Title

      The American Journal of Sports Medicine

      Volume: 46 Issue: 10 Pages: 2384-2393

    • DOI

      10.1177/0363546518781825

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] TRPV2 Protects Articular Cartilage Via Lubricin Induction2020

    • Author(s)
      Hideki Nakamoto; Fumiko Yano; Ryota Chijimatsu; Yuji Maenohara; Kosei Nagata; Sakae Tanaka; Taku Saito
    • Organizer
      ORS 2020 ANNUAL MEETING
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Thienoindazole由来低分子化合物はPI3K/AKTを介して細胞外基質産生を促進し椎間板変性を抑制する2019

    • Author(s)
      串岡純一、海渡貴司、千々松良太、矢野文子、岡田倫太郎、石黒博之、小玉城、齋藤琢、鄭雄一、吉川秀樹
    • Organizer
      第48回 脊椎脊髄病学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Runx3 は関節軟骨に保護的に作用する2019

    • Author(s)
      永田 向生,張 成虎,矢野 文子,千々松良太,牧井 勇磨,河田 学,森 大典,田中 栄,齋藤 琢
    • Organizer
      第37回骨代謝学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] メカノセンサー Trpv2 は Lubricin の誘導を介して関節軟骨を保護する2019

    • Author(s)
      中元 秀樹,千々松良太,村橋 靖崇,矢野 文子,前之原悠司,永田 向正,田中 栄,齋藤 琢
    • Organizer
      第37回骨代謝学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ルブリシンは関節軟骨最表層細胞の分化を抑制し、関節軟骨の恒常性を維持している2019

    • Author(s)
      前之原悠司,千々松良太,中元 秀樹,村橋 靖崇,矢野 文子,田中 栄,齋藤 琢
    • Organizer
      第37回骨代謝学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Tace は変形性関節症を促進的に制御する2019

    • Author(s)
      金子 泰三,千々松良太,矢野 文子,堀内 圭輔,田中 栄,齋藤 琢
    • Organizer
      第37回骨代謝学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 膝関節軟骨欠損モデルを用いたマウス滑膜細胞の軟骨再生誘導能の検討2019

    • Author(s)
      三輪哲史、千々松良太、矢野文子、田中栄、齋藤琢
    • Organizer
      第37回骨代謝学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 低分子化合物を用いた椎間板変性抑制および再生へのアプローチ2019

    • Author(s)
      串岡純一、海渡貴司、千々松良太、矢野文子、岡田倫太郎、石黒博之、Zeynep Bal、小玉城、齋藤琢、鄭雄一、吉川秀樹
    • Organizer
      第34回日本整形外科学会基礎
    • Related Report
      2019 Annual Research Report
  • [Presentation] ルブリシンは関節軟骨最表層細胞の分化を抑制し、関節軟骨の恒常性を維持している2019

    • Author(s)
      前之原悠司、千々松良太、中元秀樹、村橋靖崇、矢野文子、田中栄、齋藤琢
    • Organizer
      第34回日本整形外科学会基礎
    • Related Report
      2019 Annual Research Report
  • [Presentation] メカノセンサーTrpv2はlubricinの誘導を介して関節軟骨を保護する2019

    • Author(s)
      中元秀樹、千々松良太、村橋靖崇、矢野文子、片野坂友紀、前之原悠司、永田向正、田中栄、齋藤琢
    • Organizer
      第34回日本整形外科学会基礎
    • Related Report
      2019 Annual Research Report
  • [Presentation] Taceは変形性関節症を促進的に制御する2019

    • Author(s)
      金子泰三、千々松良太、矢野文子、堀内圭輔、田中栄、齋藤琢
    • Organizer
      第34回日本整形外科学会基礎
    • Related Report
      2019 Annual Research Report
  • [Presentation] A thienoindazole derivative small compound prevented and regenerated intervertebral disc degeneration by enhancing extracellular matrix production2018

    • Author(s)
      Junichi Kushioka, Takashi Kaito, Ryota Chijimatsu, Fumiko Yano, Rintaro Okada, Hiroyuki Ishiguro, Joe Kodama, Yuichiro Ukon, Taku Saito, Ung Chang Lee, Hideki Yoshikawa
    • Organizer
      Cervical Spine Research Society
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development Of Osteogenic Artificial Bone With Plasma Technology2018

    • Author(s)
      Joe Kodama; Ryota Chijimatsu; Yuichiro Ukon; Junichi Kushioka; Kunihiko Hashimoto; Hiroyuki Ishiguro; Rintaro Okada; Daisuke Tateiwa; Hiroyuki Tsukazaki; Takashi Kamatani; Shota Takenaka; Takahiro Makino; Yusuke Sakai; Satoshi Hamaguchi; Takashi Kaito
    • Organizer
      Orthopaedic research society
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Thienoindazole由来低分子化合物はマウスでの椎間板変性を抑制する2018

    • Author(s)
      串岡純一、海渡貴司、千々松良太、矢野文子、岡田倫太郎、石黒博之、齋藤琢、鄭雄一、吉川秀樹
    • Organizer
      脊椎脊髄病学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 脂肪由来間葉系幹細胞由来の三次元人工組織による構造的・力学的な椎間板再生2018

    • Author(s)
      石黒博之、海渡貴司、鎗光清道、牧野孝洋、武中章太、千々松良太、橋本国彦、岡田倫太郎、串岡純一、森口悠、藤江祐道、中村憲正、吉川秀樹
    • Organizer
      脊椎脊髄病学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] マウス滑膜細胞初代培養条件の検討2018

    • Author(s)
      杉本光、村橋靖崇、森大典、千々松良太、矢野文子、田中栄、齋藤琢
    • Organizer
      骨代謝学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] プラズマ表面処理を用いた官能基修飾によるバイオマテリアルの生体親和性向上2018

    • Author(s)
      大野一平、杉本敏司、伊藤智子、Anjar A.H.、小玉 城、千々松良太、海渡貴司、朝森千永子、浜口智志
    • Organizer
      プラズマ・核癒合学会
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2018-05-01   Modified: 2024-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi