Development of a simple animal species identification method for leather products by a mass spectrometer

• Project/Area Number: 18K02255
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 08030:Family and consumer sciences, and culture and living-related
• Research Institution: Okayama University
• Principal Investigator: Hatano Naoya 岡山大学, ヘルスシステム統合科学研究科, 特任講師 (10332280)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 質量分析 / ペプチド / 皮革 / 動物種鑑別

Outline of Final Research Achievements

For products made of animal leather, the Household Goods Quality Labeling Law stipulates the quality labeling of miscellaneous goods and industrial products, and for those that use even a part of this, it is essential to identify the animal species. We succeeded in extracting peptides derived from collagen protein from leather, and searched for peptides with different amino acid sequences among the 6 species of animals to be displayed. Using this as reference data for the amino acid sequence corresponding to each animal species, we constructed an "animal species determination system" that determines by combining peptide moieties with different amino acid sequences between animal species. As a result of evaluating this system in a blind test, we achieved a correct answer rate of 100% for discrimination and succeeded in constructing a simple and accurate "animal species discrimination system".

Academic Significance and Societal Importance of the Research Achievements

今回開発した皮革製品の動物種鑑別システムは、ブラインドテストにより、鑑別の正答率100%を達成した。質量分析計を用いることで、超感度に測定でき、外観を損なわない程度のわずかな試料で鑑別が可能となった。今回は表示対象の中でも特に流通量の多い6種の動物種(牛、馬、豚、羊、やぎ、鹿)を対象としたが、本手法は、その他の様々な動物種にも応用できる。製品の偽装表示問題の克服は、消費者の利益保護につながり、安心して豊かな生活を営むことができる社会の実現を可能とする。

Research Products

• [Journal Article] Regulation of the tubulin polymerization-promoting protein by Ca2+/S100 proteins (2021)
  • Author(s): Doi Seita、Fujioka Naoki、Ohtsuka Satomi、Kondo Rina、Yamamoto Maho、Denda Miwako、Magari Masaki、Kanayama Naoki、Hatano Naoya、Morishita Ryo、Hasegawa Takafumi、Tokumitsu Hiroshi
  • Journal Title: Cell Calcium Volume: 96 Pages: 102404-102404
  • DOI: 10.1016/j.ceca.2021.102404
  • Peer Reviewed / Open Access
• [Journal Article] Identification and Biochemical Characterization of High Mobility Group Protein 20A as a Novel Ca2+/S100A6 Target (2021)
  • Author(s): Yamamoto Maho、Kondo Rina、Hozumi Haruka、Doi Seita、Denda Miwako、Magari Masaki、Kanayama Naoki、Hatano Naoya、Morishita Ryo、Tokumitsu Hiroshi
  • Journal Title: Biomolecules Volume: 11 Issue: 4 Pages: 510-510
  • DOI: 10.3390/biom11040510
  • NAID: 120007037385
  • Peer Reviewed / Open Access
• [Journal Article] Both IRBIT and long-IRBIT bind to and coordinately regulate Cl -/HCO 3- exchanger AE2 activity through modulating the lysosomal degradation of AE2 (2021)
  • Author(s): Itoh, R. Hatano, N. Murakami, M. Mitsumori, K. Kawasaki, S. Wakagi, T. Kanzaki, Y. Kojima, H. Kawaai, K. Mikoshiba, K. Hamada, K. Mizutani, A.
  • Journal Title: Sci Rep . Volume: 16 Issue: 1 Pages: 5990-5990
  • DOI: 10.1038/s41598-021-85499-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Occupational respiratory allergy to lettuce in lettuce farmers (2020)
  • Author(s): Sekiya Reina、Nagano Tatsuya、Moriyama Tatsuya、Kishi Toshiyuki、Shinke Haruko、Yano Erika、Hatano Naoya、Katsurada Masahiro、Umezawa Kanoko、Katsurada Naoko、Hori Suya、Hazeki Nobuko、Fukunaga Atsushi、Yamamoto Masatsugu、Kamiryo Hiroshi、Shinohara Masakazu、Kobayashi Kazuyuki、Kotani Yoshikazu、Nishimura Yoshihiro
  • Journal Title: Clinical & Experimental Allergy Volume: 50 Issue: 8 Pages: 932-941
  • DOI: 10.1111/cea.13682
  • NAID: 120006877484
  • Peer Reviewed / Open Access
• [Journal Article] Development and Characterization of Novel Molecular Probes for Ca2+/Calmodulin-Dependent Protein Kinase Kinase, Derived from STO-609. (2020)
  • Author(s): Ohtsuka S, Ozeki Y, Fujiwara M, Miyagawa T, Kanayama N, Magari M, Hatano N, Suizu F, Ishikawa T, and Tokumitsu H.
  • Journal Title: Biochemistry Volume: 59(17) Issue: 17 Pages: 1701-1710
  • DOI: 10.1021/acs.biochem.0c00149
  • NAID: 120007027579
  • Peer Reviewed / Open Access
• [Journal Article] Phosphorylation and dephosphorylation of Ca2+/calmodulin-dependent protein kinase kinase β at Thr144 in HeLa cells (2020)
  • Author(s): Takabatake Shota、Fukumoto Yusei、Ohtsuka Satomi、Kanayama Naoki、Magari Masaki、Sakagami Hiroyuki、Hatano Naoya、Tokumitsu Hiroshi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 525 Issue: 1 Pages: 251-257
  • DOI: 10.1016/j.bbrc.2020.02.056
  • NAID: 120006961125
  • Peer Reviewed
• [Journal Article] Identification of specific protein amino acid substitutions of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli ST131: a proteomics approach using mass spectrometry (2019)
  • Author(s): Nakamura Akihiro、Komatsu Masaru、Ohno Yuki、Noguchi Nobuyoshi、Kondo Akira、Hatano Naoya
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 8555-8555
  • DOI: 10.1038/s41598-019-45051-z
  • Peer Reviewed / Open Access