DNA immobilized by photoionization of malachite green derivative and mechanism of its immobilization

• Project/Area Number: 18K05068
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 32020:Functional solid state chemistry-related
• Research Institution: Nara National College of Technology
• Principal Investigator: Uda Ryoko 奈良工業高等専門学校, 物質化学工学科, 教授 (90321463)
• Project Period (FY): 2018-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: DNA / 吸着 / 光 / マラカイトグリーン / 複合膜 / 光制御 / 光応答 / 膜 / 光イオン化

Outline of Final Research Achievements

The purpose of this work is DNA adsorption in a simple and cost-effective manner by using a malachite green derivative, which can be photoionized to afford a cationic moiety for interaction with DNA. We have prepared a polyvinyl chloride film containing a malachite green derivative and carried out photoinduced oligonucleotide adsorption on a film at the micrometer scale. We have investigated the mechanism of the DNA adsorption by using spectroscopic analysis and found that the DNA adsorption depended on the length and sequence of DNA.

Academic Significance and Societal Importance of the Research Achievements

DNAの微小領域への固定化は、マイクロアレイや電界効果トランジスタ、ラボオンチップなどに展開可能な技術である。本研究は、シンプルで柔軟性の高いDNA固定化法の構築を目指して、光でカチオンとなるユニークな性質を持つマラカイトグリーン誘導体を用いポリ塩化ビニルとの複合膜を作製した。光照射部分のみDNAを複合膜に結合させることに成功し、その結合のメカニズムを明らかにした。本研究のDNA光固定化法を用いることで、バイオチップなどの迅速な作製・研究室レベルでの自由度の高い展開が可能と考えられる。

Research Products

• [Journal Article] Photoinduced adsorption of oligonucleotides on polyvinyl chloride films containing malachite green derivative (2023)
  • Author(s): Ryoko M. Uda, Noriko Nishimoto
  • Journal Title: Soft Matter Volume: 19 Issue: 11 Pages: 2058-2063
  • DOI: 10.1039/d2sm01163h
  • Peer Reviewed
• [Journal Article] Photoinduced binding of malachite green copolymer to parallel G-quadruplex DNA (2019)
  • Author(s): Ryoko M. Uda, Noriko Nishimoto, Takashi Matsui and Shu Takagi
  • Journal Title: Soft Matter Volume: - Issue: 22 Pages: 4454-4459
  • DOI: 10.1039/c9sm00411d
  • Peer Reviewed
• [Presentation] マラカイトグリーン誘導体の光イオン化反応を用いた薄膜へのDNA吸着 (2020)
  • Author(s): 辰己 朱、西本 徳子、宇田 亮子
  • Organizer: 日本化学会第１００春季年会
• [Presentation] 光応答性マラカイトグリーン含有膜へのDNA吸着 (2019)
  • Author(s): 谷本 陸、宇田亮子
  • Organizer: 第68回高分子学会年次大会
• [Presentation] 光応答性マラカイトグリーン含有膜へのDNA吸着 (2019)
  • Author(s): 谷本 陸、宇田 亮子
  • Organizer: 第68回高分子学会年次大会
• [Book] Malachite Green: Properties and Uses, Chapter 2 (2020)
  • Author(s): R. M. Uda (Chapter 2), Rosa M. Laursen (Editor)
  • Total Pages: 161
  • Publisher: Nova Science Publishers
  • ISBN: 9781536178005