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Dynamics analysis of the nucleus by using a fluorescent labeling method for the nuclear envelope

Research Project

Project/Area Number 18K05177
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 34020:Analytical chemistry-related
Research InstitutionKyushu Institute of Technology

Principal Investigator

Sueda Shinji  九州工業大学, 大学院情報工学研究院, 教授 (00325581)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords核膜 / 核膜ラベル化 / 蛍光ラベル化 / 細胞分裂 / 核内膜タンパク質 / 核膜崩壊 / 核膜形成 / 核膜崩壊・再構成
Outline of Final Research Achievements

In this work, we have developed a fluorescent labeling method for the nuclear envelope (NE), which does not rely on the inner nuclear membrane proteins. In this method, labeling of the NE is accomplished by fixing a fluorescent protein on the inner nuclear membrane based on a unique biotinylation reaction from the archaeon Sulfolobus tokodaii. With this method, we succeeded in the tracing of the NE dynamics during cell division.

Academic Significance and Societal Importance of the Research Achievements

核膜の蛍光ラベル化技術としては核内膜上に存在するタンパク質を利用する手法が開発されているが、この場合、核内膜タンパク質がそれぞれ独自の局在性を示すため、核膜の動きを正確に追跡することができない。本研究では、古細菌由来の特殊なビオチン化酵素反応を利用して、核内膜タンパク質を利用しない、新規な蛍光ラベル化技術を開発することに成功した。本ラベル化技術は、核膜に関連した様々な生命現象の解明に活用されることが期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (12 results)

All 2021 2020 2019 2018

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (9 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results)

  • [Journal Article] Stepwise Preparation of a Polymer Comprising Protein Building Blocks on a Solid Support for Immunosensing Platform2020

    • Author(s)
      Hiroki Miyao, Utaro Uemura, and Shinji Sueda
    • Journal Title

      Analytical Sciences

      Volume: 36 Issue: 2 Pages: 213-219

    • DOI

      10.2116/analsci.19P318

    • NAID

      130007796150

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2020-02-10
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Enzyme-based protein-tagging systems for site-specific labeling of proteins in living cells2020

    • Author(s)
      Sueda Shinji
    • Journal Title

      Microscopy

      Volume: 69 Issue: 3 Pages: 156-166

    • DOI

      10.1093/jmicro/dfaa011

    • NAID

      120006997083

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fluorescent Labeling of the Nuclear Envelope by Localizing Green Fluorescent Protein on the Inner Nuclear Membrane2018

    • Author(s)
      Taniyama Toshiyuki、Tsuda Natsumi、Sueda Shinji
    • Journal Title

      ACS Chemical Biology

      Volume: 13 Issue: 6 Pages: 1463-1469

    • DOI

      10.1021/acschembio.8b00219

    • NAID

      120006630935

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 核膜選択的な蛍光ラベル化技術における核移行シグナルの機能に関する検証2021

    • Author(s)
      荒木優花、 深川凌平、都田菜摘、末田慎二
    • Organizer
      第81回分析化学討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 酵素反応を基軸としたタンパク質分析に資する蛍光ラベル化技術の開発2021

    • Author(s)
      末田慎二
    • Organizer
      第58回化学関連支部合同九州大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] IRES発現系を利用した核膜選択的な蛍光ラベル化技術の効率化2019

    • Author(s)
      荒木優花、都田菜摘、谷山俊之、末田慎二
    • Organizer
      第56回化学関連支部合同九州大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 蛍光タンパク質の細胞内局在制御技術を活用した核膜の動態解析2019

    • Author(s)
      都田菜摘、谷山俊之、末田慎二
    • Organizer
      日本分析化学会第68年会
    • Related Report
      2019 Research-status Report
  • [Presentation] 蛍光タンパク質の細胞内局在制御に基づいた核膜選択的な蛍光プローブの開発2019

    • Author(s)
      都田菜摘、谷山俊之、末田慎二
    • Organizer
      第61回日本顕微鏡学会九州支部会
    • Related Report
      2019 Research-status Report
  • [Presentation] 蛍光タンパク質の局在制御に基づいた核膜に対する選択的なラベル化技術の開発2018

    • Author(s)
      谷山俊之、都田菜摘、末田慎二
    • Organizer
      第78回分析化学討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] 細胞内での核膜の動態解析を可能にする蛍光ラベル化技術の開発2018

    • Author(s)
      都田菜摘、谷山俊之、末田慎二
    • Organizer
      第55回化学関連支部合同九州大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 古細菌由来のユニークな酵素反応系を利用した蛍光タンパク質によるラベル化技術の機能拡張2018

    • Author(s)
      末田慎二
    • Organizer
      第31回バイオメディカル分析科学シンポジウム
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Functional extension of the labeling technology with fluorescent proteins based on a unique biotinylation reaction2018

    • Author(s)
      Shinji Sueda
    • Organizer
      The 11th Japan-Korea Joint Symposium on Bio-microsensing Technology
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2018-04-23   Modified: 2022-01-27  

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