Development of a simple gene mutation detection method and verification of the frequency of drug-resistant viruses
| Project/Area Number |
18K05347
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 37030:Chemical biology-related
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
河島 尚志 東京医科大学, 医学部, 主任教授 (70224772)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | RNAウイルス / 等温核酸増幅法 / 逆転写PCR法 / Point of Care Testing / 簡易遺伝子変異検出 / 薬剤耐性ウイルス |
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| Outline of Final Research Achievements |
We constructed a detection system that can discriminate influenza mutations in about 30 minutes, although the sensitivity is low. Mutants at the site are known to be involved in the effectiveness of antiviral drugs. Furthermore, we improved this detection system, and then examined its application to simple detection of the new coronavirus. As a result, in the positive/negative judgment, this method showed a high concordance rate with the existing reverse transcription real-time PCR method (TaqMan probe method).
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| Academic Significance and Societal Importance of the Research Achievements |
本法の開発により、変異などを含む核酸バイオマーカーの簡便検出システムが構築された。よって、感染性や薬剤耐性、重篤化等を引き起こすウイルス変異の仕組みの解明や抗ウイルス薬開発の効率化等が期待できる。また、パンデミック対策への利用に加え、究極的には、それに限らず、生活習慣病やその他の感染症等様々な疾患に対する簡易診断への応用も考えられる。
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Report
(4 results)
Research Products
(19 results)
