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Development of functionalized peptide materials for 3D culture of cells and fluorescent imaging of extracellular environment.

Research Project

Project/Area Number 18K05351
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 37030:Chemical biology-related
Research InstitutionTokyo Institute of Technology

Principal Investigator

Tsutsumi Hiroshi  東京工業大学, 生命理工学院, 准教授 (70398105)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsペプチド / 自己組織化 / ヒドロゲル / 細胞 / 三次元培養 / イメージング / pH / 細胞培養
Outline of Final Research Achievements

We developed a new method to visualize extracellular environment of cancer cells cultured in 3D scaffolds composed of self-assembling peptide materials. We synthesized self-assembling peptide derivatives conjugated with a bioactive sequence (RGDS) and a pH sensitive fluorescent probe (RhoB-Aib). MCF7, a breast cancer cell, was successfully cultured in a 3D peptide hydrogel. In addition, an acidic environment of extracellular region was visualized by the fluorescent pH probe.

Academic Significance and Societal Importance of the Research Achievements

本研究は三次元的な細胞培養材料と蛍光イメージングプローブを駆使して、これまでに対象とされていなかった細胞外・細胞間の「空間」を新たなイメージング対象とする革新的な研究である。本研究は、がん細胞の悪性化の一因と考えられている細胞外酸性環境を蛍光によりリアルタイムにイメージングした最初の研究であり、がん疾患のメカニズム解明に貢献することが期待され、学術的に意義のある研究である。また、腫瘍に特有の微小環境を標的とした新しい創薬にも応用できると考えられ、社会的にも有意義な研究である。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (17 results)

All 2021 2020 2019 2018

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (13 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Journal Article] Short self‐assembling peptides with a urea bond: A new type of supramolecular peptide hydrogel materials2020

    • Author(s)
      Tsutsumi Hiroshi、Tanaka Kunifumi、Chia Jyh Yea、Mihara Hisakazu
    • Journal Title

      Peptide Science

      Volume: 113 Issue: 2 Pages: 1-13

    • DOI

      10.1002/pep2.24214

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of a Stapled α-Helix Peptide Library Displayed on Phage for the Screening of Galectin-3-Binding Peptide Ligands2020

    • Author(s)
      Anananuchatkul Teerapat、Chang Iou Ven、Miki Takayuki、Tsutsumi Hiroshi、Mihara Hisakazu
    • Journal Title

      ACS Omega

      Volume: 5 Issue: 11 Pages: 5666-5674

    • DOI

      10.1021/acsomega.9b03461

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Self-Assembling Peptides as Building Blocks of Functional Materials for Biomedical Applications2019

    • Author(s)
      Fukunaga Kazuto、Tsutsumi Hiroshi、Mihara Hisakazu
    • Journal Title

      Bulletin of the Chemical Society of Japan

      Volume: 92 Issue: 2 Pages: 391-399

    • DOI

      10.1246/bcsj.20180293

    • NAID

      130007594392

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Osteoblastic differentiation on hydrogels fabricated from Ca 2+ -responsive self-assembling peptides functionalized with bioactive peptides2018

    • Author(s)
      Tsutsumi Hiroshi、Kawamura Megumi、Mihara Hisakazu
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: 26 Issue: 12 Pages: 3126-3132

    • DOI

      10.1016/j.bmc.2018.04.039

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] 1.がん細胞外pH環境のイメージングを指向し弱酸性環境応答的蛍光プローブを組み込んだ自己集合化ペプチドゲルの創製2021

    • Author(s)
      青木 蓮・Chia Jyh Yea・三木 卓幸・三原 久和・堤 浩
    • Organizer
      日本化学会 第101春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 2.ウレア部位を有する金属イオン応答性自己集合化ペプチドの創製と金属ナノ材料への応用2021

    • Author(s)
      塩田 隼平・三木 卓幸・三原 久和・堤 浩
    • Organizer
      日本化学会 第101春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Functionalized Supramolecular Peptide Hydrogels for 3D Culture of Cancer Cells2019

    • Author(s)
      Hiroshi Tsutsumi
    • Organizer
      10th RSC-CSJ Joint Symposium
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of fluorescent pH probes toward fluorescence imaging of extracellular environments using self-assembling peptides2019

    • Author(s)
      Ren Aoki, Takayuki Miki, Hisakazu Mihara, Hiroshi Tsutsumi
    • Organizer
      第56回ペプチド討論会
    • Related Report
      2019 Research-status Report
  • [Presentation] Self-assembling peptide materials functionalized with bioactive sequences for three dimensional MCF-7 cell culture2019

    • Author(s)
      Jyh Yea Chia, Takayuki Miki, Hisakazu Mihara, Hiroshi Tsutsumi
    • Organizer
      第56回ペプチド討論会
    • Related Report
      2019 Research-status Report
  • [Presentation] ウレア結合をもつ自己集合化ペプチドゲルを用いたがん細胞の三次元培養2019

    • Author(s)
      堤 浩、Chia Jyh Yea、児玉伊織、三原久和
    • Organizer
      第29回バイオ・高分子シンポジウム
    • Related Report
      2019 Research-status Report
  • [Presentation] 自己集合化ペプチドマテリアル(1):ウレア結合をもつペプチドの構造-集合化能の相関解析と細胞培養材料応用2019

    • Author(s)
      児玉伊織・Chia Jyh Yea・三原久和・堤 浩
    • Organizer
      日本化学会 第99春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 自己集合化ペプチドマテリアル(2):がん組織工学に向けた自己集合化ペプチドヒドロゲルを用いたがん細胞の三次元培養2019

    • Author(s)
      堤 浩・Chia Jyh Yea・児玉伊織・三原久和
    • Organizer
      日本化学会 第99春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Functionalized self-assembling peptide materials with a urea bond towards the establishment of three dimensional cancer cell culture2018

    • Author(s)
      Chia Jyh Yea・児玉伊織・三原久和・堤 浩
    • Organizer
      第28回バイオ・高分子シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] ウレア結合をもつ自己組織化ペプチドの生理活性配列導入による機能化とがん細胞培養2018

    • Author(s)
      Chia Jyh Yea・児玉伊織・三原久和・堤 浩
    • Organizer
      第12回バイオ関連化学シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] Construction and Characterization of Functionalized Self-assembling Peptide Materials with a Urea Bond for Three Dimensional Cancer Cell Culture2018

    • Author(s)
      Chia Jyh Yea, Iori Kodama, Takayuki Miki, Hisakazu Mihara, Hiroshi Tsutsumi
    • Organizer
      10th International Peptide Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Construction of supramolecular peptide hydrogels functionalized with bioactive sequences for 3D cell culture2018

    • Author(s)
      Hiroshi Tsutsumi, Chia Jyh Yea, Iori Kodama, Hisakazu Mihara
    • Organizer
      10th International Peptide Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of Self-Assembling Peptide Materials Recruiting Growth Factors via Heparin Binding2018

    • Author(s)
      Hiroaki Oe, Hiroshi Tsutsumi, Takayuki Miki, Hisakazu Mihara
    • Organizer
      10th International Peptide Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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