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Genomic diversity and community structure of soybean bradyrhizobia in Philippines

Research Project

Project/Area Number 18K05376
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionUniversity of Miyazaki

Principal Investigator

Saeki Yuichi  宮崎大学, 農学部, 教授 (50295200)

Co-Investigator(Kenkyū-buntansha) 山本 昭洋  宮崎大学, 農学部, 准教授 (30452915)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsダイズ / 根粒菌 / Bradyrhizobium / Sinorhizobium / 群集構造 / 共生窒素固定 / 環境傾度 / 熱帯 / フィリピン / 塩ストレス / 親和性 / 多様性
Outline of Final Research Achievements

In this study, the diversity of indigenous bradyrhizobia collected from the soils at 11 locations in the Philippines was investigated using a local soybean cultivar. All the isolates were classified under the Bradyrhizobium species. The major influence on the distribution and diversity of soybean bradyrhizobia is attributed to the difference in the flooding period, followed by soil properties. As determined, it is proposed that the major micro-symbiont of soybean in the Philippines are B. elkanii for non-flooded soils, then B. diazoefficiens and B. japonicum for flooded soils. The most efficient and effective strain that can be used as an inoculant under the Philippines’ local condition was B. elkanii isolate in terms of symbiotic N-fixation and nodulation. In terms of their symbiotic N-fixation and nodulation under salt stress conditions, the results obtained from the mix inoculation test indicated the dominance of sinorhizobia under saline conditions.

Academic Significance and Societal Importance of the Research Achievements

現在、地球温暖化が進む中、熱帯に土着化している土壌微生物を解析することは将来の日本の農業にとっても重要な知見になる。本研究においては、ダイズと共生窒素固定を行う根粒菌について、多様性と群集構造に関して研究を行った。特にフィリピンは日本と同じ島嶼国であり、高度により温帯から熱帯気候が分布しており、温暖化の進行に伴う土壌環境を解析する研究フィールドとして適していると考えられる。本研究成果は、日本本土から南西諸島、フィリピンと根粒菌の多様性を検出し、その群集構造は緯度による差と土壌の利用形態によって遷移することを示した。温帯から熱帯地域における共生窒素固定利用について基盤となる成果である。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (16 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (6 results) Book (3 results) Remarks (1 results)

  • [Int'l Joint Research] Central Luzon State University(フィリピン)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Central Luzon State University(フィリピン)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] Central Luzon State University/Don Mariano Marcos Memorial State Univ.(フィリピン)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Effect of salt stress on soybean growth and nodulation under inoculation with soybean rhizobia2021

    • Author(s)
      Yoshikazu Nitawaki, Hayato Kitabayashi, Maria Luisa Tabing Mason, Akihiro Yamamoto, Yuichi Saeki
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 67 Issue: 2 Pages: 103-113

    • DOI

      10.1080/00380768.2020.1860644

    • NAID

      210000158746

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Symbiotic performance of indigenous soybean bradyrhizobia from the Philippines with soybean (Glycine max [L.] Merill) cultivars harboring different Rj genotypes2021

    • Author(s)
      Maria Luisa T. Mason, Baby Lyn T. De Guzman, Akihiro Yamamoto, Yuichi Saeki
    • Journal Title

      Symbiosis

      Volume: 83 Issue: 1 Pages: 55-63

    • DOI

      10.1007/s13199-020-00731-7

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Influence of flooding and soil properties on the genetic diversity and distribution of indigenous soybean-nodulating bradyrhizobia in the Philippines2018

    • Author(s)
      Maria Luisa T. Mason, Baby Lyn Cortez Tabing, Akihiro Yamamoto, Yuichi Saeki
    • Journal Title

      Heliyon

      Volume: 4 Issue: 11 Pages: e00921-e00921

    • DOI

      10.1016/j.heliyon.2018.e00921

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] アンプリコンシークエンス解析によるBradyrhizobium属ダイズ根粒菌 群集構造解析の試み2020

    • Author(s)
      平島紘子, 吉武欣之介, 山本昭洋, 佐伯雄一
    • Organizer
      日本土壌肥料学会 2020年度岡山大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 日本における土着アズキ根粒菌の群集構造に関する研究2020

    • Author(s)
      槙原 竜, 門脇正行, 佐伯雄一, 城 惣吉
    • Organizer
      日本土壌肥料学会 2020年度岡山大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 塩類集積土壌での Sinorhizobium 属根粒菌の優占化のメカニズム解明 塩類集積が根粒菌の根 粒形成遺伝子の発現に与える影響2019

    • Author(s)
      安河内孝晃, 直野晋也, 北林颯人, 山本昭洋, 佐伯雄一
    • Organizer
      日本土壌肥料学会九州支部例会
    • Related Report
      2019 Research-status Report
  • [Presentation] 塩ストレス下におけるダイズの耐塩性と感染根粒菌の群集構造2019

    • Author(s)
      北林颯人, 安河内孝晃, 山本昭洋, 佐伯雄一
    • Organizer
      日本土壌肥料学会2019年度静岡大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Genetic diversity and distribution of soybean bradyrhizobia in the Philippines2018

    • Author(s)
      Maria Luisa Tabing Mason, Akihiro Yamamoto, Yuichi Saeki
    • Organizer
      日本土壌肥料学会
    • Related Report
      2018 Research-status Report
  • [Presentation] ダイズの耐塩性と感染根粒菌の群集構造構築に関する研究 根粒菌の接種がダイズの耐塩性に与える影響およびダイズの根粒着生調節遺伝子Rfg1について2018

    • Author(s)
      北林颯人、山本昭洋、佐伯雄一
    • Organizer
      日本土壌肥料学会九州支部例会
    • Related Report
      2018 Research-status Report
  • [Book] The Soils of Japan2021

    • Author(s)
      Arakawa Y, Kusaba T, Kubotera H, Uezono I, Miyamaru N, Saeki Y, Niimi H, Nakano K, Koga N, Hara Y, Yamada Y, Inoue K, Ihara H, Nagatomo M, Yoshida K, Yamaguchi N, Hayashi H, Kondo T, Yamane T, Akagi I, Kinjo K, Shima T, Mitsugi K
    • Total Pages
      372
    • Publisher
      Springer
    • ISBN
      9789811582288
    • Related Report
      2020 Annual Research Report
  • [Book] Nitrogen Fixation(Distribution and Characterization of the Indigenous Soybean- Nodulating Bradyrhizobia in the Philippines)2020

    • Author(s)
      Maria Luisa Tabing Mason, Yuichi Saeki
    • Total Pages
      18
    • Publisher
      Intech
    • ISBN
      9781789846492
    • Related Report
      2019 Research-status Report
  • [Book] アグリバイオ(ダイズ根粒菌の生態研究を環境傾度対応型ダイズ生産技術へ)2019

    • Author(s)
      佐伯雄一・山本昭洋
    • Total Pages
      4
    • Publisher
      北隆館
    • Related Report
      2019 Research-status Report
  • [Remarks] 宮崎大学農学部応用生物科学科ホームページ

    • URL

      http://www.miyazaki-u.ac.jp/abs/

    • Related Report
      2020 Annual Research Report 2019 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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