Project/Area Number |
18K05387
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 38020:Applied microbiology-related
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Research Institution | Niigata University |
Principal Investigator |
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Project Period (FY) |
2018-04-01 – 2021-03-31
|
Project Status |
Completed (Fiscal Year 2020)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | ホスホリラーゼ / 糖 / クローニング / タンパク質発現 / 基質特異性 / 酸性オリゴ糖 / ライブラリー / 定量法 / 代謝経路 / 多糖 |
Outline of Final Research Achievements |
In order to discover and elucidate new oxidative metabolic pathways of various polysaccharides, phosphorylase that phosphorolyzes oligo-aldobionic acids, which are intermediates of oxidative degradation, has been comprehensively searched. Furthermore, the enzymatic and kinetic properties of the discovered phosphorylases were determined. Although some novel phosphorylases have been discovered, no new phosphorylases that act on oligo-aldobionic acids have been discovered. As a result of investigating the substrate specificity of the only enzyme that acted on the oligo-aldobionic acid in this search, it was a cellobionic acid phosphorylase that has a slightly different acceptor substrate specificity compared to the known one.
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Academic Significance and Societal Importance of the Research Achievements |
今回の酵素探索においては,酸化的代謝経路の中間産物である酸性オリゴ糖を加リン酸分解するホスホリラーゼを発見するに至らなかったが,いくつかの中性糖に作用する新規ホスホリラーゼを発見することはできた。当該酵素が関与するATP消費抑制型の糖質代謝経路が新たに発見された点から,学術的に意義がある。またホスホリラーゼはオリゴ糖合成の触媒としても有望であり,新規酵素が発見されたことにより新たに合成可能なオリゴ糖の種類が拡大した点も学術的に意義がある。
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