| Project/Area Number |
18K05407
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Saitama University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 分子シャペロン / 熱ショックタンパク質 / シャペロンネットワーク / シアノバクテリア / ClpB / DnaK / GroEL / DnaJ / 光合成 / 遺伝子重複 / 新規機能獲得 / DnaK（Hsp70） / J ドメインタンパク質 / シャペロニン / pH調節 |
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| Outline of Final Research Achievements |
In contrast to E.coli, cyanobacteria including Synechococcus elongatus PCC7942 contain multiple homologs (paralogs) of groEL and clpB. One of the clpB genes (clpB2) is the only example of clpB/hsp104 which is essential. E.coli has multiple dnaK genes which are not essential whereas some dnaK genes in the family are essential in cyanobacteria. DnaK functions as a central hub in the cellular molecular chaperone network. It cooperates with other molecular chaperones such as ClpB. In the present study, I focused upon unique chaperone paralogs and essential dnaK and clpB genes in S. elongatus PCC7942. As far as I know, there is no study on essential DnaK and ClpB. We established chaperone systems which consist of essential DnaK2, DnaJ1/DnaJ2, and ClpB2.
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| Academic Significance and Societal Importance of the Research Achievements |
DnaKやClpBなどの分子シャペロン（系）の研究は、主に大腸菌の（非必須）分子シャペロンやコシャペロンに関して行われてきた。本研究では、大腸菌のホモログとは生理的機能や生化学的特性が異なる（今までに研究対象とはならなかった）ホモログ（パラログ）を解析することで独自性・新規性の高い研究を行うことができた。これらの成果は、高等植物などの独立栄養生物の分子シャペロン研究に資するものと考える。
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