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Characterization of a new ability of Lactobacillus to exploit adjacent-possible ecological niche

Research Project

Project/Area Number 18K05419
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionKitakyushu National College of Technology

Principal Investigator

Mizuno Kouhei  北九州工業高等専門学校, 生産デザイン工学科, 教授 (80342583)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords乳酸菌 / ラクトバチルス / 大腸菌 / 共生 / Lactobacillus / 相互作用 / プロバイオティクス / カゼイ / ラクトバシラス / 共培養 / プラスミド / 突然変異 / Lactobacullus / 腸内細菌叢
Outline of Final Research Achievements

We discovered that a certain species of Lactobacillus in a condition could grow with co-existing E. coli. We have proposed the new inter-species interaction as Adjacent-Possible Ecological Niche(APEN) as a new concept of ecological view of point. Firstly, the plasmid derived from Listeria was found to be transformed into the Lactobacillus, which is expected to be useful for further genetic analysis. In addition, giving a fresh medium regularly, the co-culture could be kept over 1 month, which suggests that this inter-species symbiotic relation is stable. These results would give us a new insight into ecological microbiological system.

Academic Significance and Societal Importance of the Research Achievements

乳酸菌の1種ラクトバシラスは、腸管において常に少数者(0.1~数%)だが、乳酸や抗菌性物質を生産して菌叢全体を安定化させて、有害菌の侵入を防ぐ。本研究は、多くの栄養素を外部からの供給に依存する乳酸菌が、どのようにして、貧栄養環境で生存するかについて、新しい知見をもたらした。隣接する他種細菌に強力に依存して生育できるこの新しく発見された能力は、腸内環境における乳酸菌の定着や生存戦略についての定説に重要な示唆を与えるものであり、今後、腸内細菌のバランスによる疾患やその予防法などにも重要な視点を与える。
今後、本現象を分子メカニズムまで解明できれば、新しい腸内細菌叢の制御にもつながると期待できる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (16 results)

All 2020 2019 2018 Other

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Open Access: 1 results,  Peer Reviewed: 1 results) Presentation (12 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Novel multicellular prokaryote discovered next to an underground stream2020

    • Author(s)
      Kouhei Mizuno, Mais Maree, Toshihiko Nagamura, Akihiro Koga, Satoru Hirayama, Soichi Furukawa, Kenji Tanaka, Kazuya Morikawa
    • Journal Title

      Biorxiv

      Volume: nd

    • Related Report
      2020 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Journal Article] Vinylpyrroles: solid-state structures and aggregation-induced emission properties2019

    • Author(s)
      Okawara Toru、Matsufuji Yurina、Mizuno Kouhei、Takehara Kenji、Nagamura Toshihiko、Iwasa Seiji
    • Journal Title

      RSC Advances

      Volume: 9 Issue: 40 Pages: 22817-22822

    • DOI

      10.1039/c9ra04088a

    • Related Report
      2019 Research-status Report
  • [Journal Article] Production of pigments by Rhodotorula mucilaginosa2018

    • Author(s)
      Wai XianLau, Octavio Carvajal-Zarrabal, Cirilo Nolasco-Hipólito, Mizuno Kouhei,Zayn Al-Abideen Gregory, Mohammad Omar Abdullah, Seng ChiewToh, Samuel Lihan
    • Journal Title

      Malaysian Journal of microbiology

      Volume: 14 Pages: 344-350

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 乳酸菌と大腸菌の共培養に関する研究2019

    • Author(s)
      有田光騎、園田達彦、水野康平
    • Organizer
      第56回化学関連支部合同九州大会(2019年7月13日北九州市)
    • Related Report
      2019 Research-status Report
  • [Presentation] 新種細菌HS-3の形成するコロニーの形態学的基礎研究2019

    • Author(s)
      古賀暁洋、水野康平
    • Organizer
      第56回化学関連支部合同九州大会(2019年7月13日北九州市)
    • Related Report
      2019 Research-status Report
  • [Presentation] 可視光応答型光触媒による大腸菌の光殺菌効果の検討2019

    • Author(s)
      嘉村槙一、小畑賢次、水野康平、松嶋茂憲
    • Organizer
      日本セラミックス協会九州支部秋季研究発表会(2019年11月12日福岡県春日市)
    • Related Report
      2019 Research-status Report
  • [Presentation] New bacterium controls cells just like nematic liquid crystals for two-dimensional life2019

    • Author(s)
      Akihiko Koga, Mari Takahara, Tatsuhiko Sonoda, Toshihiko Nagamura, Kouhei Mizuno
    • Organizer
      第29回日本MRS年次大会(2019年11月27日横浜市)
    • Related Report
      2019 Research-status Report
  • [Presentation] Morphological changes of Lactobacillus casei in a long-term co-culture with Escherichia coli2019

    • Author(s)
      Ryo Ito, Tatsuhiko Sonoda, Kouhei Mizuno
    • Organizer
      第12回日韓合同バイオセンシングテクノロジーシンポジウム(北九州市)
    • Related Report
      2019 Research-status Report
  • [Presentation] New cave bacterium forms liquid crystal-like colony2019

    • Author(s)
      Akihiko Koga, Satoko Nakanomori, Mari Takahara, Tatsuhiko Sonoda, Toshihiko Nagamura, Kouhei Mizuno
    • Organizer
      第12回日韓合同バイオセンシングテクノロジーシンポジウム(北九州市)
    • Related Report
      2019 Research-status Report
  • [Presentation] 広域宿主プラスミドpJRD215のLactobacillus属への形質転換法の確立2018

    • Author(s)
      馬場佳也、水野康平
    • Organizer
      第55回化学関連合同支部会
    • Related Report
      2018 Research-status Report
  • [Presentation] 蛍光タンパク質遺伝子の乳酸菌への形質転換法の確立2018

    • Author(s)
      伊藤瞭、水野康平
    • Organizer
      第55回化学関連合同支部会
    • Related Report
      2018 Research-status Report
  • [Presentation] Phylogenomic analysis of catalase genes of lactic acid bacteria in the order Lactobacillales2018

    • Author(s)
      Daisuke Fukuda、Kouhei Mizuno
    • Organizer
      微生物生態学会年次大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Transformation of a red fluorescent protein DsRed into Lactobacillus casei2018

    • Author(s)
      Ryo Ito, Kouhei Mizuno
    • Organizer
      第11回日韓合同バイオマイクロセンシングテクノロジーシンポジウム
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 乳酸菌の細胞コンタクトを介した新しい環境適応能力2018

    • Author(s)
      水野康平
    • Organizer
      第115回有機デバイス研究会「有用菌の科学と産業応用」
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Two bacterial survival strategies: Adjacent-Possible ecological niche (APEN) of lactic acid bacteria, and polyhydroxyalkanoates (PHA) as a carbon and energy storage compound.2018

    • Author(s)
      水野康平
    • Organizer
      筑波大学医学セミナー
    • Related Report
      2018 Research-status Report
    • Invited
  • [Remarks] Two bacterial survival strategies: APEN of LAB...

    • URL

      http://www.md.tsukuba.ac.jp/gradmed/seminar/pdf/190123.pdf

    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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