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Identification of roles of PRMT8 with dual catalytic activity in the brain

Research Project

Project/Area Number 18K05429
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38030:Applied biochemistry-related
Research InstitutionUniversity of Toyama (2020-2021)
University of Tsukuba (2018-2019)

Principal Investigator

Jun-Dal Kim  富山大学, 学術研究部薬学・和漢系, 准教授 (90570036)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsPRMT8 / アルギニンメチル化酵素 / リン脂質加水分解酵素 / 1塩基置換マウス / アルギニンメチル化 / リン脂質加水分解 / 内因性基質 / メチル化酵素 / 神経細胞の樹状突起形成 / アセチルコリンの産生 / 脳 / CRISPR/Cas9 システム
Outline of Final Research Achievements

In this study, we established PRMT8 MTase and PLase-specific inactive mice. We also identified methylated-proteins as candidate substrate for PRMT8. In addition to this, we found that MTase, PLase, and MTase/PLase inactive mice exhibit hyperactivity under the novel environment compared to wild type mice. Interestingly, RNA-Seq analysis using the brain tissues of Prmt8 knockout mice showed that altered the regulation of neurotransmitters-related gene expression in the cerebellum.

Academic Significance and Societal Importance of the Research Achievements

本研究により、生体内におけるPRMT8の多機能を解析することは、複雑かつ多彩な生命現象とタンパク質の働きに関する理解とともに、多機能タンパク質が機能を発揮する生物学的意義の本質的な理解へ繋がる。近年、不注意や多動性、衝動性の症状を呈する最も代表的な子供の神経疾患である注意欠陥・多動性障害を持つ発達障害の患者では、12番染色体のPRMT8を含む遺伝子座に欠損変異が知られている。従って、本研究は、PRMT8が有する二酵素活性の生物学的意義の理解に留まらず、神経疾患の発症メカニズムの解明に新たな突破口として発展する可能性を秘めている。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (47 results)

All 2022 2021 2020 2019 2018

All Journal Article (15 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 15 results,  Open Access: 11 results) Presentation (32 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Multi?omics analysis of right ventricles in rat models of pulmonary arterial hypertension: Consideration of mitochondrial biogenesis by chrysin2022

    • Author(s)
      Kobayashi Takayuki、Kim Jun-Dal、Naito Akira、Yanagisawa Asako、Jujo?Sanada Takayuki、Kasuya Yoshitoshi、Nakagawa Yoshimi、Sakao Seiichiro、Tatsumi Koichiro、Suzuki Takuji
    • Journal Title

      International Journal of Molecular Medicine

      Volume: 49 Issue: 5 Pages: 69-69

    • DOI

      10.3892/ijmm.2022.5124

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Temporal transcriptomic profiling reveals dynamic changes in gene expression of Xenopus animal cap upon activin treatment2021

    • Author(s)
      Satou-Kobayashi Yumeko、Kim Jun-Dal、Fukamizu Akiyoshi、Asashima Makoto
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 14537-14537

    • DOI

      10.1038/s41598-021-93524-x

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pathophysiological Roles of Stress-Activated Protein Kinases in Pulmonary Fibrosis2021

    • Author(s)
      Kasuya Yoshitoshi、Kim Jun-Dal、Hatano Masahiko、Tatsumi Koichiro、Matsuda Shuichi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 11 Pages: 6041-6041

    • DOI

      10.3390/ijms22116041

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Starvation‐induced transcription factor CREBH negatively governs body growth by controlling GH signaling2021

    • Author(s)
      Nakagawa Yoshimi、Kumagai Kae、Han Song‐iee、Mizunoe Yuhei、Araki Masaya、Mizuno Seiya、Ohno Hiroshi、Matsuo Kazuya、Yamada Yasunari、Kim Jun‐dal、Miyamoto Takafumi、Sekiya Motohiro、Konishi Morichika、Itoh Nobuyuki、Matsuzaka Takashi、Takahashi Satoru、Sone Hirohito、Shimano Hitoshi
    • Journal Title

      The FASEB Journal

      Volume: 35 Issue: 6 Pages: 21663-21663

    • DOI

      10.1096/fj.202002784rr

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Disruption of entire Cables2 locus leads to embryonic lethality by diminished Rps21 gene expression and enhanced p53 pathway2021

    • Author(s)
      Dinh Tra Thi Huong、Iseki Hiroyoshi、Mizuno Seiya、Iijima-Mizuno Saori、Tanimoto Yoko、..., Murata Kazuya、Muratani Masafumi、Ema Masatsugu、Kim Jun-Dal、Ishida Junji、Fukamizu Akiyoshi、Kato Mitsuyasu、Takahashi Satoru、Yagami Ken-ichi、Wilson Valerie、Arkell Ruth M、Sugiyama Fumihiro
    • Journal Title

      eLife

      Volume: 10

    • DOI

      10.7554/elife.50346

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Loss of PRMT1 in the central nervous system (CNS) induces reactive astrocytes and microglia during postnatal brain development2020

    • Author(s)
      Hashimoto Misuzu、Kumabe Ayako、Kim Jun‐Dal、Murata Kazuya、Sekizar Sowmya、Williams Anna、Lu Weizhe、Ishida Junji、Nakagawa Tsutomu、Endo Mitsuharu、Minami Yasuhiro、Fukamizu Akiyoshi
    • Journal Title

      Journal of Neurochemistry

      Volume: 156 Issue: 6 Pages: 834-847

    • DOI

      10.1111/jnc.15149

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Transcriptomic Evaluation of Pulmonary Fibrosis-Related Genes: Utilization of Transgenic Mice with Modifying p38 Signal in the Lungs2020

    • Author(s)
      Matsuda Shuichi、Kim Jun-Dal、Sugiyama Fumihiro、Matsuo Yuji、Ishida Junji、Murata Kazuya、Nakamura Kanako、Namiki Kana、Sudo Tatsuhiko、Kuwaki Tomoyuki、Hatano Masahiko、Tatsumi Koichiro、Fukamizu Akiyoshi、Kasuya Yoshitoshi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 18 Pages: 6746-6746

    • DOI

      10.3390/ijms21186746

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transcriptomic changes involved in the dedifferentiation of myofibroblasts derived from the lung of a patient with idiopathic pulmonary fibrosis2020

    • Author(s)
      Suzuki Kenichi、Kim Jun-Dal、Ugai Keita、Matsuda Shuichi、Mikami Hideki、Yoshioka Kento、Ikari Jun、Hatano Masahiko、Fukamizu Akiyoshi、Tatsumi Koichiro、Kasuya Yoshitoshi
    • Journal Title

      Molecular Medicine Reports

      Volume: 22 Issue: 2 Pages: 1518-1526

    • DOI

      10.3892/mmr.2020.11218

    • NAID

      120007168384

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Histamine receptor agonist alleviates severe cardiorenal damages by eliciting anti-inflammatory programming.2020

    • Author(s)
      Noguchi, K., Ishida, J., Kim JD, Muromachi, N., Kako, K., Mizukami, H., Lu, W., Ishimaru, T., Kawasaki, S., Kaneko, Fukamizu, A
    • Journal Title

      Proc. Natl. Acad. Sci. U. S. A.

      Volume: 117 Issue: 6 Pages: 3150-3156

    • DOI

      10.1073/pnas.1909124117

    • NAID

      120007132810

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cooperative action of APJ and α1A-adrenergic receptor in vascular smooth muscle cells induces vasoconstriction2019

    • Author(s)
      Nagano K, Kwon C, Ishida J, Hashimoto T, Kim JD, Kishikawa N, Murao M, Kimura K, Kasuya Y, Kimura S, Chen YC, Tsuchimochi H, Shirai M, Pearson J, and Fukamizu A
    • Journal Title

      The Journal of Biochemistry

      Volume: 166 Issue: 5 Pages: 383-392

    • DOI

      10.1093/jb/mvz071

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The N-terminal sequence of murine PRMT5 variant 2 is required for Hsp70 interaction and CHIP ligase-mediated degradation2019

    • Author(s)
      Lu W, Kim JD, Tabara S, Kwon C, Mizukami H, Kimura K, and Fukamizu A
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 514 Issue: 4 Pages: 1185-1191

    • DOI

      10.1016/j.bbrc.2019.05.077

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Emerging impacts of biological methylation on genetic information2019

    • Author(s)
      Kako Koichiro、Kim Jun-Dal、Fukamizu Akiyoshi
    • Journal Title

      J. Biochem.

      Volume: 165 Issue: 1 Pages: 9-18

    • DOI

      10.1093/jb/mvy075

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] KDM5D-mediated H3K4 demethylation is required for sexually dimorphic gene expression in mouse embryonic fibroblasts2019

    • Author(s)
      Mizukami H, Kim JD, Tabara S, Lu W, Kwon C, Nakashima M, and Fukamizu A
    • Journal Title

      The Journal of Biochemistry

      Volume: 165 Issue: 4 Pages: 335-342

    • DOI

      10.1093/jb/mvy106

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gestational changes in PRMT1 expression of murine placentas2018

    • Author(s)
      Anna Sato, Jun-Dal Kim, Hayase Mizukami, Misaki Nakashima, Koichiro Kako, Junji Ishida, Atsuo Itakura, Satoru Takeda, Akiyoshi Fukamizu
    • Journal Title

      Placenta

      Volume: 65 Pages: 47-54

    • DOI

      10.1016/j.placenta.2018.04.001

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] PRMT1 Deficiency in Mouse Juvenile Heart Induces Dilated Cardiomyopathy and Reveals Cryptic Alternative Splicing Products2018

    • Author(s)
      Murata Kazuya、Lu Weizhe、Hashimoto Misuzu、Ono Natsumi、Muratani Masafumi、Nishikata Kana、Kim Jun-Dal、Ebihara Shizufumi、Ishida Junji、Fukamizu Akiyoshi
    • Journal Title

      iScience

      Volume: 8 Pages: 200-213

    • DOI

      10.1016/j.isci.2018.09.023

    • NAID

      120007128525

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] アクチビン処理下でのツメガエルのアニマルキャップのトランスクリプトーム解析2021

    • Author(s)
      小林夢子, 金俊達, 深水昭吉, 浅島誠
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 細胞分裂におけるSNRNP200の制御メカニズムの解析-Sororinのスプライシングと中心体制御に機能する-2021

    • Author(s)
      柳下 玲奈, 加藤 かざし, 宇田川 里奈, 村谷 匡史, 金 俊達, 木村 圭志
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 二重酵素活性を有するPRMT8の活性変異マウスの作製と機能解析2021

    • Author(s)
      森 遥佳, 金 俊達, 石田 純治, 深水 昭吉
    • Organizer
      第94回日本生化学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 心臓におけるMETTL18を介したヒスチジンメチル化の生物学的意義の解明2021

    • Author(s)
      中村 夏奈子, 金 俊達, 権 哲源, 深水 昭吉
    • Organizer
      第94回日本生化学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 遺伝子発現プロファイリングから探る、心腎連関病態のH3アゴニストによる病態改善メカニズム2021

    • Author(s)
      笠井 郁也, 石田 純治, 室町 直人, 野口 和之, 金 俊達, 深水 昭吉
    • Organizer
      第94回日本生化学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Study on the effect of H179Y one-point mutation in murine PRMT1 on biological functions2021

    • Author(s)
      姚 遠, 金 俊達, 大徳 浩照, 室町 直人, 石田 純治, 深水 昭吉
    • Organizer
      第94回日本生化学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 脂肪組織形成における METTL18 を介したヒスチジンメチル化に関する研究2021

    • Author(s)
      岸川 奈那, 金 俊達, 石田 純治, 深水 昭吉
    • Organizer
      第94回日本生化学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 高血圧妊娠胎仔FGRの遺伝子発現の特質解析2021

    • Author(s)
      石田 純治, 松田 紘奈, 金 俊達, 深水 昭吉
    • Organizer
      第94回日本生化学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] PRMT1欠損は生後の脳においてアストロサイトやミクログリアの活性化を引き起こす2021

    • Author(s)
      橋本美涼, 金 俊達, 村田知弥, 中川寅, 深水昭吉
    • Organizer
      第85回日本生化学会中部支部例会・シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] Study on the effect of H179Y one-point mutation in murine PRMT1 on biological functions2020

    • Author(s)
      姚遠, 室町直人, 金俊達, 深水昭吉
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] ヒスタミンH3受容体アゴニストの心腎病態での遺伝子発現プロファイリング2020

    • Author(s)
      室町直人, 金俊達, 石田純治, 野口和之, 深水昭吉
    • Organizer
      第93回日本生化学会大会 2
    • Related Report
      2020 Research-status Report
  • [Presentation] PRMT8が有するアルギニンメチル化活性の生物学的意義に関する研究2020

    • Author(s)
      森遥佳, 田原早央莉, 金俊達, 深水昭吉
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 心筋細胞特異的遺伝子欠損マウスを用いたMETTL18の機能解析2020

    • Author(s)
      中村夏奈子, 金俊達, 権哲源, 深水昭吉
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 宇宙マウス心臓を用いた遺伝情報の統合的解析2020

    • Author(s)
      田原早央莉, 金俊達, 陸偉哲, 深水昭吉
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 心腎連関病態モデルマウスの確立と解析2019

    • Author(s)
      野口 和之、石田 純治、室町直人、金 俊達、加香 孝一郎、山縣 邦弘、深水 昭吉
    • Organizer
      第62回日本腎臓学会学術総会
    • Related Report
      2019 Research-status Report
  • [Presentation] 妊娠高血圧モデルの肥大心を用いた網羅的遺伝し発言解析とα1A-ARの役割の解明2019

    • Author(s)
      権哲源、中村夏奈子、田原早央莉、金俊達、石田純治、深水 昭吉
    • Organizer
      第40回日本妊娠高血圧学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] PRMT8が有する2つの酵素活性に対する特異的不活性マウスの作製と解析2019

    • Author(s)
      田原早央莉、金俊達、森遥佳、陸偉哲、深水 昭吉
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 妊娠高血圧胎仔FGRの遺伝子発現プロファイリング2019

    • Author(s)
      石田純治、金俊達、霍思全、中島実咲、深水昭吉
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] PRMT8が有するアルギニンメチル化活性の生物学的意義に関する研究2019

    • Author(s)
      田原早央莉、金俊達、 森遥佳、陸偉哲、深水 昭吉
    • Organizer
      2019年度日本化学会関東支部例会
    • Related Report
      2019 Research-status Report
  • [Presentation] Hsp70/CHIPsによるPRMT5バリアン2のN末端配列依存的な分解機構2019

    • Author(s)
      陸偉哲、金俊達、 田原早央莉、権哲源、深水 昭吉
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ヒスタミンH3受容体アゴニストの心腎病態でに抗炎症遺伝子プログラミング2019

    • Author(s)
      室町直人、石田純治、野口和之、金俊逹、深水 昭吉
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 血管平滑筋細胞のアペリン受容体が誘導する毛感攣縮の解析2019

    • Author(s)
      権哲源、永野克将、金俊逹、石田純治、深水 昭吉
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] The Role of α1A-Adrenergic Receptor on Cardiac Function in Pregnancy-Associated Hypertensive Mice2019

    • Author(s)
      Chulwon Kwon, Kanako Nakamura, Saori Tabara, Jun-Dal Kim, Junji Ishida, Akiyoshi Fukamizu
    • Organizer
      46th Annual Meeting & International Symposium, The Korean Society for Microbiology and Biotechnology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 血管平滑筋細胞のアペリン受容体が誘導する毛感攣縮の解析2019

    • Author(s)
      権哲源、永野克将、金俊逹、石田純治、深水昭吉
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 妊娠高血圧/α1A-AR-KOマウスの肥大心臓では、Hmgcs2の発現が変化する2019

    • Author(s)
      権 哲源、中村 夏奈子、田原 早央莉、金 俊逹、石田 純治、深水 昭吉
    • Organizer
      2019年度 日本生化学会関東支部例会
    • Related Report
      2019 Research-status Report
  • [Presentation] Analysis of Transcription and Translation by RNA-seq and Ribo-seq in Cardiac PRMT1-Deficient Mice2018

    • Author(s)
      Weizhe Lu, Kazuya Murata, Hayase Mizukami, Jun-dal Kim, Junji Ishida, Akiyoshi Fukamizu
    • Organizer
      2018年生化学会関東支部例会
    • Related Report
      2018 Research-status Report
  • [Presentation] PRMT1スプライスバリアント欠損がマウス個体の代謝に与える影響2018

    • Author(s)
      水上早瀬、金 俊逹、陸 偉哲、横山 航、中島実咲、石田純治、深水昭吉
    • Organizer
      2018年生化学会関東支部例会
    • Related Report
      2018 Research-status Report
  • [Presentation] PRMT8のアルギニンメチル化に対する不活性化マウスの作製と標的基質の探索2018

    • Author(s)
      田原早央莉、金 俊達、水上早瀬、陸 偉哲、深水昭吉
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 次世代シーケンサーを用いて心筋PRMT1欠損マウス心臓遺伝子の転写と翻訳解析2018

    • Author(s)
      陸 偉哲、村田知弥、水上早瀬、金 俊達、石田純治、深水昭吉
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 糖代謝におけるPRMT1v2の役割の解明2018

    • Author(s)
      水上早瀬、金 俊逹、陸 偉哲、中島実咲、石田純治、深水昭吉
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Gene Expression Landscape of Heart Failure Mice model caused by Cardiac Specific PRMT1 Deficiency2018

    • Author(s)
      Weizhe Lu, Kazuya Murata, Hayase Mizukami, Jun-dal Kim, Junji Ishida, Akiyoshi Fukamizu
    • Organizer
      第2回日本循環器学会基礎研究フォーラム
    • Related Report
      2018 Research-status Report
  • [Presentation] Gestational changes in placental PRMT1 expression2018

    • Author(s)
      Anna Sato, Jun-Dai Kim, Akiyoshi Fukamizu, Atsuo Itakura, Satoru Takeda
    • Organizer
      Meeting of the International Federation of Placenta Associations (IFPA) 2018 Tokyo
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2023-01-30  

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