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Synthesis of the adsorbent imitated from the binding site of biomolecules and its application to mycotoxin analysis

Research Project

Project/Area Number 18K05526
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38050:Food sciences-related
Research InstitutionChubu University

Principal Investigator

Yamamoto Atsushi  中部大学, 応用生物学部, 教授 (60360806)

Co-Investigator(Kenkyū-buntansha) 小玉 修嗣  東海大学, 理学部, 教授 (70360807)
山本 雅納  東北大学, 多元物質科学研究所, 助教 (70802966)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords環境負荷因子 / ハサミ型吸着剤 / リュープロレリン / 環境負荷危害因子 / オクラトキシンA / 特異的吸着剤 / 人工抗体 / カビ毒 / 理論化学計算 / 酵素可溶化法
Outline of Final Research Achievements

The binding constant of the peptide, leuprorelin to mycotoxin, ochratoxin A in acetonitrile was about 10,000 /M at 0 degree Celsius. However, its value of the leuprorelin immobilized resin to ochratoxin was exceeding 100,000,000 /M. This value is comparable to that of ochratoxin A antibody, and it is speculated that two leuprorelin functional groups in close proximity include one ochratoxin A molecule in cooperation. We have shown for the first time that immobilization of a peptide in a bimolecular competitive arrangement produces specific adsorption comparable to that of an antibody. We pursued the possibility of equivalent collaborative adsorption with smaller molecular functional groups.

Academic Significance and Societal Importance of the Research Achievements

特異的吸着現象の代名詞として使われているのが「抗体」である。生成には生体の防御反応を利用せざるを得ず、動物愛護の見地からは決して望ましいものではない。これと同等の吸着特性を有する化学合成品として高分子アプタマが知られているが、より小さな分子が協働で作用することでも可能であることを見出したのは世界初である。また、今回のコロナ禍においてワクチン開発技術が脚光を浴びているが、ワクチン投与によって生体内で抗体を誘導するのではなく、治療薬としての人工抗体の開発にも応用可能な技術となりうる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (8 results)

All 2021 2020 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] A label-free, direct solid-phase fluorimetric analysis of ochratoxin A in agricultural products with monoclonal antibody-immobilized monolith2021

    • Author(s)
      Koji Kikukawa, Miki Fukui, Ayano Oka, Ryohei Yamamoto, Atsushi Yamamoto, Mikiko Uchigashima, Takeshi Kono, Daisuke Kozaki, Shuji Kodama
    • Journal Title

      Food Chemistry

      Volume: 346 Pages: 128736-128736

    • DOI

      10.1016/j.foodchem.2020.128736

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Probing the Entropic Effect in Molecular Noncovalent Interactions between Resin-Bound Polybrominated Arenes and Small Substrates2018

    • Author(s)
      Masanori Yamamoto, Miyuki Obara, Keisuke Ochi, Atsushi Yamamoto, KatsuhikoTakenaka, Tsunehiro Tanaka, Kazunori Sato
    • Journal Title

      ChemPlusChem

      Volume: 83 Issue: 9 Pages: 820-824

    • DOI

      10.1002/cplu.201800304

    • NAID

      120006818708

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 分子間作用型吸着剤を用いたジチオカルバメート系農薬の分析法2020

    • Author(s)
      小出高羅、菊川浩史、中島花奈、山本良平、山本 敦
    • Organizer
      第80回分析化学討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ジチオカルバメート系農薬分析法の問題点とその解決策2020

    • Author(s)
      小出高羅、菊川浩史、山口瑛紀、山本良平、山本 敦
    • Organizer
      日本分析化学会第69年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 吸着剤官能基の導入密度と捕捉特性に関する考察2020

    • Author(s)
      小久保翠月、山本 敦
    • Organizer
      第20回高山フォーラム
    • Related Report
      2020 Annual Research Report
  • [Presentation] ハロゲン化フェノール類の特異的吸着剤の開発2020

    • Author(s)
      小久保翠月、小出高羅、山本良平、山本 敦、三輪俊夫、林 義貴
    • Organizer
      日本薬学会第140年会
    • Related Report
      2019 Research-status Report
  • [Presentation] 血清アルブミンのリガンド結合能を利用したカビ毒ochratoxin Aのワンポット精製法とその分析への応用2019

    • Author(s)
      大和直樹、冨澤千明、山本良平、山本 敦、小玉修嗣
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Research-status Report
  • [Presentation] A simple and rapid pretreatment of coffee beans with serum albumin for ochratoxin A HPLC analysis2018

    • Author(s)
      Naoki Yamato, Atsumi Nagaya, Ryohei Yamamoto, Atsushi Yamamoto, Naoya Kasai
    • Organizer
      Association for the Science and Information on Coffee
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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