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Identification of the responsible genes that govern Na and Cl accumulation in leaves of rice under salinity stress

Research Project

Project/Area Number 18K05572
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39010:Science in plant genetics and breeding-related
Research InstitutionShinshu University

Principal Investigator

Horie Tomoaki  信州大学, 学術研究院繊維学系, 教授 (90591181)

Co-Investigator(Kenkyū-buntansha) 石川 亮  神戸大学, 農学研究科, 准教授 (70467687)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords耐塩性 / イネ / 遺伝資源 / Na+輸送 / Cl-輸送
Outline of Final Research Achievements

We searched for genetic loci that govern leaf Na accumulation in rice under salt stress using backcross recombinant inbred lines derived from cultivated rice and wild rice (composed of 159 lines). A quantitative trait locus for Na accumulation was deduced to exist in Chromosome 1 and 5, respectively. However, the responsible genes have not yet been isolated. On the other hand, chromosome segment substitution lines (CSSLs) derived from cultivated rice and salt tolerant rice (composed of 44 lines) were used to search for important loci controlling Cl accumulation in rice under salt stress. Several unexpected problems have hindered the progression of research for Cl. Trial and error has, however, led to the assumption that root Cl accumulation could be an important index to consider salt tolerance of rice. Based on the root-based anion analysis using CSSLs, at least one genetic locus that significantly influences on the level of root Cl accumulation was identified.

Academic Significance and Societal Importance of the Research Achievements

NaやClの高蓄積を伴う土壌の塩類集積は、アジアやアフリカでの稲作と米収量に深刻な悪影響を及ぼし始めている。栽培イネは、穀類で最も塩ストレスに脆弱であり、耐塩性に優れた栽培品種を作出することが急務である。本研究では、塩ストレス環境下における主たる毒性要素であるNaやClの、イネ体内での分配や蓄積に大きな影響を及ぼすと予想される遺伝子座領域を発見することができた。今後、それらの責任遺伝子の構造や機能を同定することで有用知見を蓄積させ、耐塩性のイネ栽培品種の育種を加速させることができるかもしれない。本研究の成果は、その礎となり得るものである。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (8 results)

All 2021 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results) Presentation (3 results) Remarks (1 results)

  • [Int'l Joint Research] タスマニア大学(オーストラリア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] タスマニア大学(オーストラリア)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Functions and structure of roots and their contributions to salinity tolerance in plants2021

    • Author(s)
      Ichirou Karahara, Tomoaki Horie
    • Journal Title

      Breeding Science

      Volume: 71 Issue: 1 Pages: 89-108

    • DOI

      10.1270/jsbbs.20123

    • NAID

      130007991336

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Doing 'business as usual' comes with a cost: evaluating energy cost of maintaining plant intracellular K+ homeostasis under saline conditions.2019

    • Author(s)
      Rubio F, Nieves-Cordones M, Horie T, Shabala S.
    • Journal Title

      New Phytologist

      Volume: - Issue: 3 Pages: 1097-1104

    • DOI

      10.1111/nph.15852

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 塩ストレス下のイネの葉身のNa+濃度を制御するQTLの探索2021

    • Author(s)
      戸田 楓香, 石川 亮, Amarasinghe Yaddehige Priya Jayantha, 石井 尊生, 堀江 智明1)
    • Organizer
      日本育種学会 第140回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 野生イネ(O. rufipogon)-栽培イネ(O. sativa)CSSLsを利用した塩ストレス下のイネのCl-恒常性機構を制御する遺伝子座の探索2019

    • Author(s)
      佐藤くるみ、高師知紀、伊藤隆、堀江智明
    • Organizer
      日本育種学会 第136回講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] 塩ストレス下のイネのCl-輸送・分配システムの解明を目指したCl-蓄積量の評価2018

    • Author(s)
      佐藤くるみ、伊藤隆、石川亮、石井尊生、堀江智明
    • Organizer
      日本育種学会 第134回講演会
    • Related Report
      2018 Research-status Report
  • [Remarks] 信州大学学術情報オンラインシステムSOAR

    • URL

      http://soar-rd.shinshu-u.ac.jp/profile/ja.OFTmWUDU.html

    • Related Report
      2020 Research-status Report 2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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