Identification of a disease resistant gene in anthesis of rice. Does it block the attack of the pathogen on the surface of stigma after the pollination?

• Project/Area Number: 18K05581
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Mizobuchi Ritsuko 国立研究開発法人農業・食品産業技術総合研究機構, 作物研究部門, グループ長補佐 (40425591)
• Project Period (FY): 2018-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: イネ / 病害抵抗性 / もみ枯細菌病 / Burkholderia glumae

Outline of Final Research Achievements

The objective of this study is cloning　and characterization the QTL (RBG2) for resistance to bacterial grain rot, caused by the bacterial pathogen Burkholderia glumae, which is a destructive disease in heading stage of rice. We analyzed the CSSLs derived from a cross between Koshihikari　(susceptible) and cultivars of World Rice Collection. We found that a line derived from cultivar A was resistant. The expression of RBG2 on the surface of stigma was detected in both of K (resistant) and H (susceptible).

Academic Significance and Societal Importance of the Research Achievements

本研究では、もみ枯細菌病菌に対する穂枯れ抵抗性遺伝子の単離と機能解明を目指し、コアコレクション品種の染色体を「コシヒカリ」(罹病性)に導入した染色体置換系統（CSSLs）から、品種AのRBG2領域をコシヒカリに導入した系統が穂枯れ抵抗性を示すことを明らかにした。このことは、世界の複数の品種のRBG2アリルが今後育種母本として有用であることを示唆している。もみ枯細菌病の穂枯れ抵抗性遺伝子を単離して機能を明らかにした報告はないため、本研究成果は、学術的および育種的に重要な知見であり、今後は本研究で見出した予測遺伝子が真の原因遺伝子であるかどうかを明らかにしていく予定である。

Research Products

• [Journal Article] A MAPKKK gene from rice, RBG1res, confers resistance to Burkholderia glumae through negative regulation of ABA (2023)
  • Author(s): Mizobuchi Ritsuko、Sugimoto Kazuhiko、Tsushima Seiya、Fukuoka Shuichi、Tsuiki Chikako、Endo Masaki、Mikami Masafumi、Saika Hiroaki、Sato Hiroyuki
  • Journal Title: Scientific Reports Volume: 13 Issue: 1 Pages: 3947-3947
  • DOI: 10.1038/s41598-023-30471-9
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of major rice cultivars for resistance to bacterial seedling rot caused by <i>Burkholderia glumae</i> and identification of Japanese standard cultivars for resistance assessments (2020)
  • Author(s): Mizobuchi Ritsuko、Fukuoka Shuichi、Tsuiki Chikako、Tsushima Seiya、Sato Hiroyuki
  • Journal Title: Breeding Science Volume: 70 Issue: 2 Pages: 221-230
  • DOI: 10.1270/jsbbs.19117
  • NAID: 130007833734
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed / Open Access
• [Presentation] 日本の主要水稲品種のもみ枯細菌病菌による苗腐敗抵抗性の評価 (2019)
  • Author(s): 溝淵律子、福岡修一、對木千加子、對馬誠也、佐藤宏之
  • Organizer: 日本育種学会