| Project/Area Number |
18K05640
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 39040:Plant protection science-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
Sone Teruo 北海道大学, 農学研究院, 教授 (00333633)
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| Co-Investigator(Kenkyū-buntansha) |
阿部 歩 北海道大学, 農学研究院, 技術専門職員 (70374626)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | イネいもち病 / 薬剤耐性 / 突然変異 / ミトコンドリア / GFP / QoI / 損傷乗り越え合成 / オルタナティブオキシダーゼ / QoI剤 / eGFP / mitotracker |
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| Outline of Final Research Achievements |
This study aimed to clarify the mechanism of emergence, inheritance and distribution of QoI fungicide resistance in rice blast fungus. We could not conclude about the mechanism of mutation emergence, due to the difficulty of mutation induction in vitro. On the other hand, visualization of mitochondria with GFP was succeed, and it enabled to observe that only a few mitochondria were distributed to the conidia as tubule form, and they are divided into dots simultaneously with the septum formation between conidia and conidiophore. It suggested us that conidiation has a role for the rapid distribution of the mutant.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究はイネいもち病の防除において大きな問題となっているQoI剤耐性の発生と蔓延の仕組みを明らかにしようとするものである．本研究により菌の中で少量発生した変異ミトコンドリアが，胞子に分配される際に，急速に自己増殖し，変異株になる過程が明らかにされた．即ち，ミトコンドリアの自己増殖を防ぐことで，変異の蔓延を遅らせる事が出来る可能性が示唆された．また，菌の中のミトコンドリアを可視化する技術や，変異ミトコンドリアを定量する技術も開発され，いもち病菌におけるミトコンドリア生物学の基礎が確立された．
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