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Dynamism on pathogen recognition and subsequent responses through the plant cell wall

Research Project

Project/Area Number 18K05645
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionOkayama University

Principal Investigator

TOYODA Kazuhiro  岡山大学, 環境生命科学研究科, 教授 (50294442)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords植物免疫 / 植物細胞壁 / エリシター / サプレッサー / オキシダティブバースト / 防御応答 / 活性酸素種(ROS) / 細胞壁ペルオキシダーゼ / 細胞膜 NADPH オキシダーゼ / シロイヌナズナ PRX34 / スルフェン酸化 / シロイヌナズナ / PRX34 / 細胞壁 / ペルオキシダーゼ / 活性酸素種 / 病原体認識 / エフェクター
Outline of Final Research Achievements

In recent years, research on plant immunity (PTI) through pattern recognition receptors (PRRs) has progressed, but its relationship (interaction) with signal transduction originating from the plant cell wall remains unknown. We have previously shown that the plant cell wall directly recognizes pathogens through constituent(s) and initiates intracellular and extracellular defense responses. We also made efforts on a molecular mechanism by which pathogen- and plant-derived suppressors attenuate elicitor/MAMP-induced defense responses. In this study, we found a role of the plant cell wall during the PTI response, and demonstrated the existence of an unknown signaling pathway that does not require known molecules.

Academic Significance and Societal Importance of the Research Achievements

植物の病原体感染時における認識と応答についての一連の研究から、植物細胞壁の重要性が改めて明らかとなった。特に細胞壁に分泌されるペルオキシダーゼが活性酸素種(ROS)生成だけでなくカビなどによる侵入の障壁となるカロース生成・蓄積に必須であることが示された。事実、ペルオキシダーゼ欠損個体では病原菌に対する罹病性は増加し、逆に過剰発現個体には抵抗性が付与された。本知見はペルオキシダーゼ(遺伝子)が植物の耐病性品種作出の上で新たなターゲット分子となることを示しており、学術的意義に留まらず応用的にも価値がある。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (38 results)

All 2021 2020 2019 2018

All Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (32 results) (of which Int'l Joint Research: 3 results) Book (1 results)

  • [Journal Article] Antagonism between SA- and JA- signaling conditioned by saccharin in Arabidopsis renders resistance to a specific pathogen2020

    • Author(s)
      Phuong, L.T., Fitrianti, A.N., Luan, M.T., Matsui, H., Noutoshi, Y., Yamamoto, M., Ichinose, Y., Shiraishi, T. and Toyoda, K.
    • Journal Title

      J. Gen. Plant Pathol.

      Volume: 86(2) Issue: 2 Pages: 86-99

    • DOI

      10.1007/s10327-019-00899-x

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Endogenous suppressor(s) in Arabidopsis thaliana2020

    • Author(s)
      Mai, T.L., Kawasaki, T., Fitrianti, A.N., Phuong, L.T., Shiokawa, T., Tada, H., Matsui, H., Noutoshi, Y., Yamamoto, M., Ichinose, Y., Shiraishi, T. and Toyoda, K.
    • Journal Title

      J. Gen. Plant Pathol.

      Volume: 86(2) Issue: 2 Pages: 100-106

    • DOI

      10.1007/s10327-019-00897-z

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The plant activator saccharin induces resistance to wheat powdery mildew through activation of multiple defense-related genes2020

    • Author(s)
      Phuong, L.T., Zhao, L., Fitrianti, A.N., Matsui, H., Noutoshi, Y., Yamamoto, M., Ichinose, Y., Shiraishi, T. and Toyoda, K.
    • Journal Title

      J. Gen. Plant Pathol.

      Volume: 86(2) Issue: 2 Pages: 107-113

    • DOI

      10.1007/s10327-019-00900-7

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A class III peroxidase PRX34 is a component of disease resistance in Arabidopsis2019

    • Author(s)
      1)Zhao, L., Phuong, L.T., Luan, M.T., Fitrianti, A.N., Matsui, H., Nakagami, H., Noutoshi, Y., Yamamoto, M., Ichinose, Y., Shiraishi, T. and Toyoda, K.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 85 Issue: 6 Pages: 405-412

    • DOI

      10.1007/s10327-019-00863-9

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A class III peroxidase PRX34 is a component of disease resistance in Arabidopsis2019

    • Author(s)
      1)Lei Zhao, Le Thi Phuong, Mai Thanh Luan, Aprilia Nur Fitrianti, Hidenori Matsui, Hirofumi Nakagami, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi and Kazuhiro Toyoda
    • Journal Title

      Journal of General Plant Pathology

      Volume: 印刷中

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] The role of CEP5 peptide as an endogenous suppressor in Arabidopsis thaliana2021

    • Author(s)
      Aprilia Nur Fitrianti, Thanh Luan Mai, Tsugumi Shiokawa, Hiroko Tada, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, Kazuhiro Toyoda
    • Organizer
      International Symposium on Environmental and Life Science: FY2020 Debrief Meeting on Education and Research (25 January 2021), Okayama University, Okayama, Japan
    • Related Report
      2020 Annual Research Report
  • [Presentation] メトミノストロビンの抵抗性誘導作用に関する研究 (3) メトミノストロビンはシロイヌナズナの MAP キナーゼを活性化する2021

    • Author(s)
      伊藤千晶・小原七海・佐藤穂高・市成光広・山田 晶・櫻本和生・白石 慎・山本 隆・松井英譲・能年義輝・山本幹博・一瀬勇規・白石友紀・豊田和弘
    • Organizer
      日本農薬学会第46回大会(令和3年3月8~10日),東京農工大学(オンライン),東京
    • Related Report
      2020 Annual Research Report
  • [Presentation] メトミノストロビンの抵抗性誘導作用に関する研究(4) メトミノストロビンはシロイヌナズナにおいて PTI 関連遺伝子の発現を促進する2021

    • Author(s)
      小原七海・伊藤千晶・佐藤穂高・市成光広・山田 晶・櫻本和生・白石 慎・山本 隆・松井英譲・能年義輝・山本幹博・一瀬勇規・白石友紀・豊田和弘
    • Organizer
      日本農薬学会第46回大会(令和3年3月8~10日),東京農工大学(オンライン),東京
    • Related Report
      2020 Annual Research Report
  • [Presentation] CEP5 peptide negatively modulates defense responses in Arabidopsis thaliana2021

    • Author(s)
      Aprilia, N.F., Luan, M.T., Matsui, H., Noutoshi, Y., Yamamoto, M., Ichinose, Y., Shiraishi, T., Toyoda, K.
    • Organizer
      令和3年度日本植物病理学会大会(令和3年3月18~20日),津(オンライン)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] メトミノストロビンの抵抗性誘導作用に関する研究5.シロイヌナズナのPTI応答における MAPキナーゼ活性に対する影響2021

    • Author(s)
      伊藤千晶・小原七海・佐藤穂高・市成光広・山田 晶・櫻本和生・白石 慎・山本 隆・松井英譲・能年義輝・山本幹博・一瀬勇規・白石友紀・豊田和弘
    • Organizer
      令和3年度日本植物病理学会大会(令和3年3月18~20日),津(オンライン)
    • Related Report
      2020 Annual Research Report
  • [Presentation] メトミノストロビンの抵抗性誘導作用に関する研究 6.シロイヌナズナにおける PTI 関連遺伝子の発現に対する影響2021

    • Author(s)
      小原七海・伊藤千晶・佐藤穂高・市成光広・山田 晶・櫻本和生・白石 慎・山本 隆・松井英譲・能年義輝・山本幹博・一瀬勇規・白石友紀・豊田和弘
    • Organizer
      令和3年度日本植物病理学会大会(令和3年3月18~20日),津(オンライン)
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナの細胞内/細胞外レドックス制御における NADPH オキシダーゼ RBOHD の役割2020

    • Author(s)
      高須瑞穂・木元菜々子・松井英譲・能年義輝・山本幹博・一瀬勇規・白石友紀・豊田和弘
    • Organizer
      令和2年度日本植物病理学会関西部会(令和2年11月7~8日),島根大学(オンライン),松江
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナの MAMP 誘導性オキシダティブバーストにおける細胞壁ペルオキシダーゼと NADPH オキシダーゼの関与2020

    • Author(s)
      木元菜々子・高須瑞穂・Aprilia Nur Fitrianti・松井英譲・能年義輝・山本幹博・一瀬勇規・白石友紀・豊田和弘
    • Organizer
      令和2年度日本植物病理学会関西部会(令和2年11月7~8日),島根大学(オンライン),松江
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナにおける既知の受容体を介さないカビの認識機構2020

    • Author(s)
      矢野裕奈・Aprilia Nur Fitrianti・木元菜々子・松井英譲・能年義輝・山本幹博・一瀬勇規・白石友紀・豊田和弘
    • Organizer
      令和2年度日本植物病理学会関西部会(令和2年11月7~8日),島根大学(オンライン),松江
    • Related Report
      2020 Annual Research Report
  • [Presentation] The role of CEP5 peptide as an endogenous suppressor in Arabidopsis thaliana2020

    • Author(s)
      Aprilia, N.F., Luan, M.T., Matsui, H., Noutoshi, Y., Yamamoto, M., Ichinose, Y., Shiraishi, T., Toyoda, K.
    • Organizer
      令和2年度日本植物病理学会関西部会(令和2年11月7~8日),島根大学(オンライン),松江
    • Related Report
      2020 Annual Research Report
  • [Presentation] Endogenous suppressor(s) in Arabidopsis thaliana2020

    • Author(s)
      Aprilia Nur Fitrianti, Mai Thanh Luan, Le Thi Phuong, Lei Zhao, Hiori Monden, Norika Shiiba, Tsugumi Shiokawa, Hiroko Tada, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi and Kazuhiro Toyoda
    • Organizer
      2020 Japan-US Early Career Online Symposium (29 September to 7 October), Cornell University, Ithaca, NY, USA
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Endogenous suppressor(s) in Arabidopsis thaliana2019

    • Author(s)
      1)Mai Thanh Luan, Tatsuhiro Kawasaki, Aprilia Nur Fitrianti, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi and Kazuhiro Toyoda
    • Organizer
      Vietnam-Japan Science and Technology 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Study on the mode of action of CEP peptide in Arabidopsis2019

    • Author(s)
      2)Aprilia Nur Fitrianti, Mai Thanh Luan, Le Thi Phuong, Lei Zhao, Hiyori Monden, Norika Shiiba, Tsugumi Shiokawa, Hiroko Tada, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi and Kazuhiro Toyoda
    • Organizer
      Kansai-area Meeting of the Phytopathological Society of Japan
    • Related Report
      2019 Research-status Report
  • [Presentation] The mode of action of saccharin as a plant activator of disease resistance2019

    • Author(s)
      3)Le Thi Phuong, Mai Thanh Luan, Aprilia Nur Fitrianti, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi, and Kazuhiro Toyoda
    • Organizer
      Kansai-area Meeting of the Phytopathological Society of Japan
    • Related Report
      2019 Research-status Report
  • [Presentation] メトミノストロビンの作用機構に関する研究Ⅱ.コムギにおけるうどんこ病抵抗性のプライミング2019

    • Author(s)
      4)佐藤穂高・Le Thi Phuong・市成光広・櫻本和生・山田 晶・松井英譲・能年義輝・山本幹博・一瀬勇規・白石友紀・豊田和弘
    • Organizer
      令和元年度日本植物病理学会関西部会
    • Related Report
      2019 Research-status Report
  • [Presentation] シロイヌナズナの分泌型ペルオキシダーゼ PRX34 は MAMP 誘導性オキシダティブバーストに関与する2019

    • Author(s)
      5)高須瑞穂・木元菜々子・Zhao Lei・Le Thi Phuong・松井英譲・能年義輝・山本幹博・一瀬勇規・白石友紀・豊田和弘
    • Organizer
      令和元年度日本植物病理学会関西部会
    • Related Report
      2019 Research-status Report
  • [Presentation] シロイヌナズナにおける既知の受容体を必要としない病原糸状菌の認識2019

    • Author(s)
      6)矢野裕奈・アプリリア ヌ フィランティ・松井英譲・能年義輝・山本幹博・一瀬勇規・白石友紀・豊田和弘
    • Organizer
      令和2年度日本植物病理学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] シロイヌナズナにおけるオキシダティブバースト反応の前提条件:細胞内/細胞外レドックス制御における NADPH オキシダーゼ RBOHD の役割2019

    • Author(s)
      7)高須瑞穂・木元菜々子・松井英譲・能年義輝・山本幹博・一瀬勇規・白石友紀・豊田和弘
    • Organizer
      令和2年度日本植物病理学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] シロイヌナズナの MAMP 誘導性オキシダティブバースト反応に対する細胞壁ペルオキシダーゼと NADPH オキシダーゼの寄与2019

    • Author(s)
      8)木元菜々子・高須瑞穂・アプリリア ヌ フィランティ・ザオ レイ・松井英譲・能年義輝・山本幹博・一瀬勇規・白石友紀・豊田和弘
    • Organizer
      令和2年度日本植物病理学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Antagonism between SA- and JA-signaling conditioned by saccharin renders resistance to a specific pathogen in Arabidopsis thaliana2018

    • Author(s)
      1)Le Thi Phuong, Aprilia Nur Fitrianti, Mai Thanh Luan, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi and Kazuhiro Toyoda
    • Organizer
      The 29th Annual Meeting of the Japanese Plant-Microbe Interactions (19-21 September 2018)
    • Related Report
      2018 Research-status Report
  • [Presentation] A Cep peptide acts as an endogenous suppressor in Arabidopsis thaliana2018

    • Author(s)
      2)Aprilia Nur Fitrianti, Mai Thanh Luan, Le Thi Phuong, Lei Zhao, Tsugumi Shiokawa, Hiroko Tada, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi and Kazuhiro Toyoda
    • Organizer
      The 29th Annual Meeting of the Japanese Plant-Microbe Interactions (19-21 September 2018)
    • Related Report
      2018 Research-status Report
  • [Presentation] エンドウの細胞壁タンパク質に検出されるキトサン結合タンパク質2018

    • Author(s)
      3)松尾実佳、川端真矢、三木紅葉、松井英譲、能年義輝、山本幹博、一瀬勇規、白石友紀、豊田和弘
    • Organizer
      植物微生物研究会第28回研究交流会(平成30年9月19~21日)
    • Related Report
      2018 Research-status Report
  • [Presentation] Antagonism between SA- and JA-signaling conditioned by saccharin renders resistance to a specific pathogen in Arabidopsis thaliana2018

    • Author(s)
      4)Le Thi Phuong, Aprilia Nur Fitrianti, Mai Thanh Luan, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi and Kazuhiro Toyoda,
    • Organizer
      Kansai-area Meeting of the Phytopathological Society of Japan (27-28 September 2018)
    • Related Report
      2018 Research-status Report
  • [Presentation] エンドウの細胞壁タンパク質に検出されるキトサン結合タンパク質2018

    • Author(s)
      5)松尾実佳、川端真矢、三木紅葉、松井英譲、能年義輝、山本幹博、一瀬勇規、白石友紀、豊田和弘
    • Organizer
      平成30年度日本植物病理学会関西部会(平成30年9月27~28日)
    • Related Report
      2018 Research-status Report
  • [Presentation] 細胞外オキシダティブバースト反応の分子機構:ペルオキシダーゼのスルフェン酸化とその役割2018

    • Author(s)
      6)高須瑞穂、吉岡美樹、吉岡博文、松井英譲、能年義輝、山本幹博、一瀬勇規、白石友紀、豊田和弘
    • Organizer
      平成30年度日本植物病理学会関西部会(平成30年9月27~28日)
    • Related Report
      2018 Research-status Report
  • [Presentation] A CEP peptide acts as an endogenous suppressor in Arabidopsis2018

    • Author(s)
      7)Aprilia Nur Fitrianti, Mai Thanh Luan, Le Thi Phuong, Lei Zhao, Tsugumi Shiokawa, Hiroko Tada, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi and Kazuhiro Toyoda
    • Organizer
      Kansai-area Meeting of the Phytopathological Society of Japan (27-28 September 2018)
    • Related Report
      2018 Research-status Report
  • [Presentation] メトミノストロビンの作用機構に関する研究2018

    • Author(s)
      8)佐藤穂高、市成光広、櫻本和生、山田 晶、松井英譲、能年義輝、山本幹博、一瀬勇規、白石友紀、豊田和弘
    • Organizer
      平成30年度日本植物病理学会関西部会(平成30年9月27~28日)
    • Related Report
      2018 Research-status Report
  • [Presentation] A class III peroxidase PRX34 is a component of disease resistance in Arabidopsis2018

    • Author(s)
      9)Lei Zhao, Le Thi Phuong, Mai Thanh Luan, Aprilia Nur Fitrianti, Hidenori Matsui, Hirofumi Nakagami, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi and Kazuhiro Toyoda
    • Organizer
      Jilin University-Okayama University Joint Sakura Science Workshop (18 December 2018)
    • Related Report
      2018 Research-status Report
  • [Presentation] エンドウの細胞壁画分に見出されたキトサンと結合するタンパク質複合体2018

    • Author(s)
      10)松尾実佳、三木紅葉、松井英譲、能年義輝、山本幹博、一瀬勇規、白石友紀、豊田和弘
    • Organizer
      平成31年度日本植物病理学会大会(平成31年3月18~20日)
    • Related Report
      2018 Research-status Report
  • [Presentation] AtCEP peptide promotes disease susceptibility towards non-adapted pathogens in Arabidopsis thaliana2018

    • Author(s)
      11)Aprilia Nur Fitrianti, Mai Thanh Luan, Le Thi Phuong, Lei Zhao, Hiori Monden, Norika Shiiba, Tsugumi Shiokawa, Hiroko Tada, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi and Kazuhiro Toyoda
    • Organizer
      2019 Annual Meeting of the Phytopathological Society of Japan (18-20 March 2019)
    • Related Report
      2018 Research-status Report
  • [Presentation] A class III peroxidase PRX34 is a component of disease resistance in Arabidopsis2018

    • Author(s)
      12)Lei Zhao, Le Thi Phuong, Mai Thanh Luan, Aprilia Nur Fitrianti, Hidenori Matsui, Hirofumi Nakagami, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi and Kazuhiro Toyoda
    • Organizer
      2019 Annual Meeting of the Phytopathological Society of Japan (18-20 March 2019)
    • Related Report
      2018 Research-status Report
  • [Presentation] Saccharin induces wheat resistance to powdery mildew fungus through activation of defense genes2018

    • Author(s)
      13)Le Thi Phuong, Mai Thanh Luan, Lei Zhao, Aprilia Nur Fitrianti, Hidenori Matsui, Yoshiteru Noutoshi, Mikihiro Yamamoto, Yuki Ichinose, Tomonori Shiraishi and Kazuhiro Toyoda
    • Organizer
      2019 Annual Meeting of the Phytopathological Society of Japan (18-20 March 2019)
    • Related Report
      2018 Research-status Report
  • [Book] Ultrastructural and cytological studies on Mycosphaerella pinodes infection of the model legume Medicago truncatula (doi: 10.3389/978-2-88945-634-5), In Advances in Ascochyta Research2018

    • Author(s)
      1)Tomoko Suzuki, Aya Maeda, Masaya Hirose, Yuki Ichinose, Tomonori Shiraishi and Kazuhiro Toyoda(分担)
    • Total Pages
      193
    • Publisher
      Frontiers Media SA, Switzerland
    • ISBN
      9782889456345
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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