Fusarium wilt resistance induced by magnesium oxide in Fusarium wilt-susceptible tomato plants

• Project/Area Number: 18K05647
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: Yamaguchi University
• Principal Investigator: Ito Shin-ichi 山口大学, 大学院創成科学研究科, 教授 (30243629)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Fusarium oxysporum / トマト萎ちょう病 / 誘導抵抗性 / 植物免疫 / 酸化マグネシウム / ナノ粒子 / ジャスモン酸 / トマト / Ｆｕｓａｒｉｕｍ ｏｘｙｓｐｏｒｕｍ

Outline of Final Research Achievements

Treatment of roots of Fusarium wilt-susceptible tomato plants with magnesium oxide (MgO) containing nanoparticles induces strong Fusarium wilt-resistance in the plants. The aim of this study was to elucidate the mechanism of the Fusarium wilt-resistance induced by MgO in tomato plants. The study suggested that the jasmonic acid signaling pathway is essential for the establishment of the MgO-induced resistance against Fusarium wilt in tomato. In addition, it was suggested that both DAMP-triggered immunity (DTI) and PAMP-triggered immunity (PTI) are involved in the resistance. Since the resistance was maintained until fruit harvest, MgO treatment of tomato seedlings is expected to be a practical technology for the control of Fusarium wilt of tomato.

Academic Significance and Societal Importance of the Research Achievements

1. 学術的意義：本研究により以下のことが初めて明らかになった。①萎ちょう病感受性トマトが萎ちょう病抵抗性になるメカニズム。②萎ちょう病感受性トマトに萎ちょう病抵抗性を誘導する方法。③MgO処理によるトマトの遺伝子発現パターン再構成と、病原菌感染に対する迅速応答。④MgOによるトマトの複数の植物ホルモンシグナル伝達経路の活性化。
2. 社会的意義：本研究によって、安価で安全な天然素材であるMgOがトマト萎ちょう病の防除に利用できることが明らかになった。トマト萎ちょう病を含むFusarium萎ちょう病防除技術の確立は世界的な課題であることから、本研究成果の社会的意義はきわめて大きい。

Research Products

• [Int'l Joint Research] Karnatak University(インド)
• [Int'l Joint Research] Prince of Songkla University(タイ)
• [Journal Article] Magnesium oxide induces immunity against Fusarium wilt by triggering the jasmonic acid signaling pathway in tomato (2021)
  • Author(s): Fujikawa Isamu、Takehara Yushi、Ota Makiko、Imada Kiyoshi、Sasaki Kazunori、Kajihara Hiroshi、Sakai Shoji、Jogaiah Sudisha、Ito Shin-ichi
  • Journal Title: Journal of Biotechnology Volume: 325 Pages: 100-108
  • DOI: 10.1016/j.jbiotec.2020.11.012
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] MgO-induced defence against bacterial wilt disease in Arabidopsis thaliana. (2019)
  • Author(s): Ota, M., Imada, K., Sasaki, K., Kajihara, H., Sakai, S., Ito, S.
  • Journal Title: Plant Pathology Volume: 68 Issue: 2 Pages: 323-333
  • DOI: 10.1111/ppa.12939
  • Peer Reviewed
• [Presentation] タマネギ乾腐病菌の日本株およびヨーロッパ株間の遺伝的多様性 (2021)
  • Author(s): 佐々木一紀・國本雅晃・執行正義・伊藤真一
  • Organizer: 令和3年度日本植物病理学会大会（オンライン開催、三重市）（2021月3月17日～19日）
• [Presentation] トマト根腐萎凋病菌のMC69ホモログの病原性への関与 (2020)
  • Author(s): 伊藤由后・濱田侑希・伊藤真一・佐々木一紀
  • Organizer: 令和2年度日本植物病理学会関西部会（オンライン開催、松江市）（2020月11月7日～8日）
• [Presentation] Suppression of Fusarium wilt of tomato by magnesium oxide nanoparticles (MgO NP) (2019)
  • Author(s): S. Ito
  • Organizer: International Conference on Emerging Advancement in Science & Technology.New Delhi, India (5-6 September, 2019)
  • Int'l Joint Research / Invited
• [Presentation] 仮焼酸化マグネシウムが誘導するトマト萎凋病発病抑制の解析 (2019)
  • Author(s): 藤川勇・佐々木一紀・境昭二・伊藤真一
  • Organizer: 日本植物病理学会大会