| Project/Area Number |
18K05662
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 39040:Plant protection science-related
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
Yoshida Shigenobu 国立研究開発法人農業・食品産業技術総合研究機構, 植物防疫研究部門, グループ長 (90354125)
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| Co-Investigator(Kenkyū-buntansha) |
山内 智史 国立研究開発法人農業・食品産業技術総合研究機構, 中日本農業研究センター, 上級研究員 (50442760)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | バイオフィルム / バイオコントロール / 複合種 / 生物的防除 |
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| Outline of Final Research Achievements |
To obtain fundamental knowledge on the development of the novel biocontrol strategy by enhancing biofilm (BF) formation of biocontrol agents on plant surface using BF formation promoting microbes, we investigated bacterial strains enhancing BF formation together with Pseudomonas sp. strain 62AP4, which shows inhibitory effect against gray mold of tomato caused by Botrytis cinerea. Consequently, we found the bacterial strain 62HD-25, obtained from culture collection of tomato phyllosphere, stably enhanced BF formation with strain 62AP4, and verified optimal co-inoculation condition of both strains for the formation. When potted tomato plants were co-inoculated with both bacterial strains, then inoculated with B. cinerea, occurrence of gray mold symptom was stably suppressed on the leaf surface, and this suppression was suggested owing to inhibition of conidial germination of B. cinerea by BF formed by co-inoculation with strains 62AP4 and 62HD-25.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果は、ある一定の発病抑制ポテンシャルを持つ微生物株に他の微生物種との協同体を形成させ、植物体上での定着性を増強させて抑制効果を安定化させようとする、農作物病害の新たな生物的防除技術開発の実現可能性を提示する基盤的成果であるだけでなく、微生物生態学的観点でも異種微生物間の相互作用の理解増進と新たな機能解明に役立つ成果でもあることから、その学術的な意義は大きい。また、本成果は「みどりの食料システム戦略」に代表される近年の我が国の農林水産行政施策の目標の一つとなっている化学農薬に代替しうる病害防除技術の新たな開発に資する成果でもあることから、社会的にも大きな意義を持つものである。
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