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Development of DNA markers to identify races of southern root-knot nematode.

Research Project

Project/Area Number 18K05663
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

Tabuchi Hiroaki  国立研究開発法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター, 上級研究員 (10355571)

Co-Investigator(Kenkyū-buntansha) 浅水 恵理香  龍谷大学, 農学部, 教授 (00370924)
上杉 謙太  国立研究開発法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター, 主任研究員 (00414798)
鈴木 崇之  国立研究開発法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター, チーム長 (20414823)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsサツマイモネコブセンチュウ / レース / DNAマーカー / サツマイモ
Outline of Final Research Achievements

Races of the southern root-knot nematode (SRKN) is identified based on their different responses to six differential sweetpotato cultivars. Because this inoculation method is time-comsuming and laboriuos, we intented to develop DNA markers to identify SRKN races easily. At first, we established new single egg-mass isolates of SRKN followed by the race idenfication with inocultaion method. Then we conducted DNA polymorphism analysis of 40 new and already-existing isolates of SRKN, and did not identify the race-specific genome region. However, we suppose that analysis of about 20 isolates of each SRKN race might restrict the race-specific DNA region to several sites out of about fifty thousand polymorphic sites.

Academic Significance and Societal Importance of the Research Achievements

線虫レースの分化に関与するゲノム領域の同定は、植物が線虫を異物と認識し、線虫がそれを回避するために分子進化する機構の解明に繋がる。また、簡易なレース判別システムの開発により、抵抗性品種の導入による低コストな線虫被害抑制対策が行える。本研究では各レースにつき約20系統の解析によりレース特異的DNA多型部位を数カ所程度まで絞り込める可能性を示すことで、上記目標に向けた新知見を与えるものである。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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