| Project/Area Number |
18K05821
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 40040:Aquatic life science-related
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
金久保 光央 国立研究開発法人産業技術総合研究所, 材料・化学領域, 主任研究員 (70286764)
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| Project Period (FY) |
2018-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | コラーゲン / オリーブ葉 / 線維芽細胞 / ポリフェノール / オリーブハマチ |
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| Outline of Final Research Achievements |
We investigated the causes of the increase in muscle collagen content in cultured yellowtail muscle due to the ingestion of olive leaf powder using fibroblast cells. When olive leaf extract was added to fibroblast cells, the following three changes occurred: (1) the intracellular glutathione concentration was increased, and vitamin C, which is essential for collagen synthesis, was retained in high level for a long time, (2) the expression of genes coding the following three proteins was raised: type I collagen, enzyme involved in post-translational modification of collagen (POLD1), and protein involved in collagen folding (HSP47), (3) the activity of collagen-degrading enzymes was decreased. It was speculated that the intake of olive leaf powder to fish increases the amount of muscle collagen due to the combined effects of the above three factors.
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| Academic Significance and Societal Importance of the Research Achievements |
生体内でのコラーゲンの合成能力は年齢とともに衰えていく。コラーゲン合成を促進する物質はビタミンＣなど数種類しか報告がない。オリーブ葉粉末の抽出物にもコラーゲン合成促進効果があること、その作用機序がビタミンＣの作用機序とは異なるところに学術的な意義がある。オリーブ葉抽出物はヒト由来の線維芽細胞でもコラーゲン産生を促進することから、オリーブ葉抽出物は加齢によるコラーゲンの合成能力の低下を抑制できる可能性を秘めており、社会的な意義は大きい。
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