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The mechanism of fish carbon dioxide anaesthesia to alleviate pain

Research Project

Project/Area Number 18K05830
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionKanazawa University

Principal Investigator

Matsubara Hajime  金沢大学, 生命理工学系, 教授 (50459715)

Co-Investigator(Kenkyū-buntansha) 渡邉 研一  東京農業大学, 生物産業学部, 教授 (20426315)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords魚類麻酔 / 炭酸 / ストレス / 可視化 / 魚類福祉 / 炭酸麻酔作用機序 / ストレス可視化 / 炭酸ガス / ストレス軽減 / 体表色彩 / 背地適応 / 両側回遊 / 炭酸取り込み / 炭酸麻酔 / 麻酔作用機序 / 苦痛軽減
Outline of Final Research Achievements

It is unclear whether fish reduce the pain same as the anesthetic action (suppress nerve activity such as the brain and central nervous system) of mammal. Because fish are poikilotherm and respirate with the help of gills. In this study, we artificially caused stress in the backland-adapted fish Japanese flounder using potassium. And we succeeded in quantifying the change in body surface color, which is the first stress response. In addition, we investigated well-known stress factors as well. It was clarified that carbonic dioxide anesthesia is the most stress-relieving fish anesthesia.

Academic Significance and Societal Importance of the Research Achievements

麻酔に関する知見は哺乳類では豊富であるが、魚類の多くは水棲変温動物であり、代謝や呼吸中枢の酸素圧低下に対する耐性などがそれと異なるため、哺乳類の麻酔剤が使えないことも多い。本研究により、「炭酸」が、他の既存麻酔剤に比べ、ストレスを軽減することを見出した。この成果は現存するストレス軽減魚類麻酔剤として炭酸を各機関に紹介し、魚類福祉に大きく貢献できると確信している。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (26 results)

All 2021 2020 2019 2018

All Journal Article (13 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 13 results,  Open Access: 5 results) Presentation (12 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Glyoxal‐induced formation of advanced glycation end‐products in type 1 collagen decreases both its strength and flexibility in vitro2021

    • Author(s)
      Kitamura Kei‐ichiro、Hirayama Jun、Tabuchi Yoshiaki、Minami Takao、Matsubara Hajime、Hattori Atsuhiko、Suzuki Nobuo
    • Journal Title

      Journal of Diabetes Investigation

      Volume: 12 Issue: 9 Pages: 1555-1559

    • DOI

      10.1111/jdi.13528

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression dynamics of the genes for the hypothalamo-pituitary-gonadal axis in tiger puffer (Takifugu rubripes) at different reproductive stages2021

    • Author(s)
      Zahangir Md. Mahiuddin、Matsubara Hajime、Ogiso Shouzo、Suzuki Nobuo、Ueda Hiroshi、Ando Hironori
    • Journal Title

      General and Comparative Endocrinology

      Volume: 301 Pages: 113660-113660

    • DOI

      10.1016/j.ygcen.2020.113660

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] ヤツメウナギ類(カワヤツメ、シベリアヤツメ)の好中球の形態学的および細胞化学的特徴2021

    • Author(s)
      近藤昌和, 安本信哉, 松原創
    • Journal Title

      水産大学校研究報告

      Volume: 69 Pages: 41-49

    • NAID

      120007089480

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of sclerostin in the regenerating scales of goldfish and its increase under microgravity during space flight2020

    • Author(s)
      Yamamoto, T., Ikegame, M., Hirayama, J., Kitamura, K., Tabuchi, Y., Furusawa, Y., Sekiguchi, T., Endo, M., Mishima, H., Seki, A., Yano, S., Matsubara H., Hattori, A. and Suzuki, N.
    • Journal Title

      Biomedical Research

      Volume: 41 Issue: 6 Pages: 279-288

    • DOI

      10.2220/biomedres.41.279

    • NAID

      130007948844

    • ISSN
      0388-6107, 1880-313X
    • Year and Date
      2020-12-01
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detection of RANKL-producing cells and osteoclastic activation by the addition of exogenous RANKL in the regenerating scales of goldfish.2020

    • Author(s)
      Yamamoto, T., Ikegame, M., Kawago, U., Tabuchi, Y., Hirayama, J., Sekiguchi, T., Furusawa, Y., Yachiguchi, K., Matsubara, H., Urata, M., Hattori, A. and Suzuki, N.
    • Journal Title

      Biological Science in Space

      Volume: 34 Issue: 0 Pages: 34-40

    • DOI

      10.2187/bss.34.34

    • NAID

      130007884615

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Morphologies and population genetic structures of the eight-barbel loach of the genus Lefua on southern Sakhalin2020

    • Author(s)
      Machida Yoshiyasu、Kanaiwa Minoru、Shedko Sergey V.、Matsubara Hajime、Kobayashi Hirozumi、Mandagi Ixchel F.、Ooyagi Akira、Yamahira Kazunori
    • Journal Title

      Ichthyological Research

      Volume: 68 Issue: 2 Pages: 239-248

    • DOI

      10.1007/s10228-020-00783-1

    • NAID

      40022547174

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Oral administration of melatonin contained in drinking water increased bone strength in naturally aged mice2020

    • Author(s)
      Igarashi-Migitaka Junko、Seki Azusa、Ikegame Mika、Honda Masato、Sekiguchi Toshio、Mishima Hiroyuki、Shimizu Nobuaki、Matsubara Hajime、Srivastav Ajai K.、Hirayama Jun、Maruyama Yusuke、Kamijo-Ikemori Atsuko、Hirata Kazuaki、Hattori Atsuhiko、Suzuki Nobuo
    • Journal Title

      Acta Histochemica

      Volume: 122 Issue: 6 Pages: 151596-151596

    • DOI

      10.1016/j.acthis.2020.151596

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata2020

    • Author(s)
      Zanaty Mohamed I.、Sawada Niina、Kitani Yoichiro、Nassar Hossam F.、Mahmoud Hamada M.、Hayakawa Kazuichi、Sekiguchi Toshio、Ogiso Shouzo、Tabuchi Yoshiaki、Urata Makoto、Matsubara Hajime、Takeuchi Yutaka、Hattori Atsuhiko、Srivastav Ajai K.、Amornsakun Thumronk、Suzuki Nobuo
    • Journal Title

      International Journal of Environmental Research and Public Health

      Volume: 17 Issue: 4 Pages: 1391-1391

    • DOI

      10.3390/ijerph17041391

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Changes in Sex Steroids and Ovarian Steroidogenic Enzyme mRNA Levels in Artificially Maturing Japanese Eel (Anguilla japonica) and Naturally Maturing New Zealand Longfin Eel (Anguilla dieffenbachii) during Vitellogenesis2019

    • Author(s)
      Matsubara Hajime、Lokman P. Mark、Kazeto Yukinori、Okumura Hiromi、Ijiri Shigeho、Hirai Toshiaki、Young Graham、Adachi Shinji、Yamauchi Kohei
    • Journal Title

      Fishes

      Volume: 4 Issue: 4 Pages: 52-52

    • DOI

      10.3390/fishes4040052

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gadolinium at Low Concentration Suppresses both Osteoclastic and Osteoblastic Activities in the Scales of Goldfish2019

    • Author(s)
      Suzuki Nobuo、Watanabe Kazuki、Sekimoto Aika、Urata Makoto、Zanaty Mohamed Ibrahim、Sekiguchi Toshio、Kitani Yoichiro、Matsubara Hajime、Srivastav Ajai Kumar、Hattori Atsuhiko
    • Journal Title

      American Journal of Environmental Sciences

      Volume: 15 Issue: 4 Pages: 137-144

    • DOI

      10.3844/ajessp.2019.137.144

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Physiological Role of a Calcitonin-Like Substance in the Life Cycle of the Lamprey, Lampetra japonica (Cyclostomata)2019

    • Author(s)
      Nobuo Suzuki、Toshio Sekiguchi、Koji Yachiguchi、Makoto Urata、Yasuko Kinoshita、Hajime Matsubara、Kiyoshi Asahina、Akira Kambegawa、Ajai Kumar Srivastav
    • Journal Title

      International Journal of Zoological Investigations

      Volume: 05 Issue: 02 Pages: 23-30

    • DOI

      10.33745/ijzi.2019.v05i02.003

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Development of a system for measuring calcitonin in the stingray Dasyatis akajei (a cartilaginous fish): the possible involvement of stingray calcitonin in gonadal development2019

    • Author(s)
      Takagi Takashi、Sekiguchi Toshio、Sasayama Yuichi、Hattori Atsuhiko、Watanabe Takushi X.、Kitani Yoichiro、Tabuchi Yoshiaki、Matsubara Hajime、Srivastav Ajai K.、Hai Tran Ngoc、Amornsakun Thumronk、Suzuki Nobuo
    • Journal Title

      International Aquatic Research

      Volume: 11 Issue: 3 Pages: 267-276

    • DOI

      10.1007/s40071-019-00236-0

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Infection status of commercial fish with cystacanth larvae of the genus Corynosoma (Acanthocephala: Polymorphidae) in Hokkaido, Japan2019

    • Author(s)
      Sasaki Mizuki、Katahira Hirotaka、Kobayashi Mari、Kuramochi Toshiaki、Matsubara Hajime、Nakao Minoru
    • Journal Title

      International Journal of Food Microbiology

      Volume: 305 Pages: 108256-108256

    • DOI

      10.1016/j.ijfoodmicro.2019.108256

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 深海性高級魚キチジの種苗生産に関する研究-VI2019

    • Author(s)
      松原創,春見達郎,岩田大輔,小林寛,永見新,横田高士,中野美和,松原孝博,鈴木信雄,柳町隆造
    • Organizer
      日本水産学会春季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ニホンザリガニの生殖腺形態の周年変化2019

    • Author(s)
      小林寛,松原創,永見新,鈴木信雄,中田和義
    • Organizer
      日本水産学会春季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 海水魚と淡水魚精子の卵門侵入2019

    • Author(s)
      松原創,春見達郎,小林寛,永見新,横田高士,中野美和,市村政樹,近藤昌和,鈴木信雄,柳町隆造
    • Organizer
      日本水産学会春季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 魚類における炭酸ガスの麻酔作用2019

    • Author(s)
      松原創,小林寛,重松惇志,永見新,近藤昌和,鈴木信雄
    • Organizer
      日本水産学会秋季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] シシャモの初期発生を制御する環境要因2019

    • Author(s)
      林寛,高橋優太,大倉俊介,重松惇志,永見新,中尾勝哉,川崎琢真,山口浩志,横田高士,中野美和,春見達朗,鈴木信雄,松原創
    • Organizer
      日本水産学会秋季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 深海性高級魚キチジの種苗生産に関する研究-V2019

    • Author(s)
      松原創、春見達郎、岩田大輔、小林寛、永見新、横田高士、中野美和、松原孝博、鈴木信雄、柳町隆造
    • Organizer
      平成31年度日本水産学会春期大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 海水魚と淡水魚精子の卵門侵入2019

    • Author(s)
      松原創、春見達郎、小林寛、永見新、横田高士、中野美和、市村政樹、近藤昌和、鈴木信雄、柳町隆造
    • Organizer
      平成31年度日本水産学会春期大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ニホンザリガニの生殖腺形態の周年変化2019

    • Author(s)
      小林寛、松原創、永見新、鈴木信雄、中田和義
    • Organizer
      平成31年度日本水産学会春期大会
    • Related Report
      2018 Research-status Report
  • [Presentation] General aquaculture in Japan2018

    • Author(s)
      Hajime Matsubara
    • Organizer
      Joint Symposium between Kanazawa University and Prince of Songkla University on Recent Advances in Marine Science, Aquaculture and Food Technology
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Fish fertilization -Fish sperm into egg through the micropyle -2018

    • Author(s)
      Hajime Matsubara, Tatsuro Harumi, Arata Nagami, Hiroshi Kobayashi,Masakazu Kondoh, Masaki Ichimura and Ryuzo Yanagimachi
    • Organizer
      Joint Symposium between Kanazawa University and Prince of Songkla University on Recent Advances in Marine Science, Aquaculture and Food Technology
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] The carbon dioxide as fish anesthetic2018

    • Author(s)
      Arata Nagami,Hiroshi,Kobayashi, Masakazu Kondoh and Hajime Matsubara
    • Organizer
      Joint Symposium between Kanazawa University and Prince of Songkla University on Recent Advances in Marine Science, Aquaculture and Food Technology
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Reproduction of endangered Japanese crayfish, Cambaroides japonicus2018

    • Author(s)
      Hiroshi Kobayashi, Arata Nagami, Masakazu Kondoh, Kazuyoshi Nakata and Hajime Matsubara
    • Organizer
      Joint Symposium between Kanazawa University and Prince of Songkla University on Recent Advances in Marine Science, Aquaculture and Food Technology
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Book] 魚類学の百科事典2018

    • Author(s)
      日本魚類学会編集
    • Total Pages
      718
    • Publisher
      丸善出版株式会社
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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