Elucidation of the transmission and diffusion mechanism of antibacterial resistance in Salmonella that contaminates broilers

• Project/Area Number: 18K05997
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Kagoshima University
• Principal Investigator: Chuma Takehisa 鹿児島大学, 農水産獣医学域獣医学系, 教授 (90201631)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: サルモネラ / 抗菌剤耐性 / ブロイラー

Outline of Final Research Achievements

In this study, we isolated Salmonella from the cecal contents of broilers in Kagoshima prefecture, investigated serotype and antimicrobial resistance, and attempted to elucidate the mode of the transmission. From 2018 to 2021, the Salmonella positive rates from individual chickens showed an increasing trend of 12.5, 13.4, 21.6 and 23.6%, respectively, and the positive rates of chicken flock were 72.9%, 72.9%, 89.6% and 93.8%, respectively. As a result of antimicrobial susceptibility testing, ABPC, CTX, and CFX resistance were no longer observed in 2018. On the other hand, from 2018 to 2020, the isolation rate of S. Schwarzengrund increased and KM resistance increased, strongly suggesting a relationship between serotype and resistance acquisition.

Academic Significance and Societal Importance of the Research Achievements

食中毒の原因菌であり近年では薬剤耐性菌の出現も問題視されているサルモネラのブロイラー農場レベルにおける汚染状況が明らかとなった。サルモネラの抗菌剤感受性が近年大きく変化している傾向が認められ、今後の薬剤使用のための有力な情報となった。サルモネラの抗菌剤耐性獲得のメカニズムとして血清型との関わりが示され、抗菌剤耐性菌の蔓延防止につながる可能性がある。

Research Products

• [Journal Article] Decreasing trend of β-lactam resistance in Salmonella isolates from broiler chickens due to the cessation of ceftiofur in ovo administration (2022)
  • Author(s): Duc V M, Kakiuchi R, Muneyasu H, Toyofuku H, Obi T, Chuma T
  • Journal Title: Veterinary and Animal Science Volume: 16 Pages: 1002481-5
  • DOI: 10.1016/j.vas.2022.100248
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Increased Salmonella Schwarzengrund prevalence and antimicrobial susceptibility of Salmonella enterica isolated from broiler chickens in Kagoshima Prefecture in Japan between 2013 and 2016 (2020)
  • Author(s): Duc V M, Shin J, Nagamatsu Y, Fujiwara A, Toyofuku H, Obi T, Chuma T.
  • Journal Title: Journal of Veterinary Medical Science Volume: 82 Pages: 585-589
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prevalence of Salmonella in broiler chickens in Kagoshima, Japan in 2009 to 2012 and the relationship between serovars changing and antimicrobial resistance (2019)
  • Author(s): Duc V. M., Nakamoto Y., Fujiwara A., Toyofuku H., Obi T., and Chuma T.
  • Journal Title: BMC Veterinary Research Volume: 15 Issue: 1 Pages: 108-115
  • DOI: 10.1186/s12917-019-1836-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 2019、2020年に鹿児島県のブロイラーから分離されたサルモネラの抗菌剤耐性 (2021)
  • Author(s): 守富健人、Phan Thi Thuy Linh、津留優、宗安祥佳、Vu Minh Duc、山元三保子、中馬猛久
  • Organizer: 令和3年度獣医学術九州地区学会
• [Presentation] The disappearance of β-lactam resistance in Salmonella isolates from broiler chickens in Kagoshima Prefecture in 2018. (2020)
  • Author(s): Vu Minh Duc、宗安祥佳、豊福肇、小尾岳士、中馬猛久
  • Organizer: 第163回日本獣医学会
• [Presentation] Antimicrobial resistance genes of Salmonella serovars isolated from broiler chickens in Kagoshima, Japan (2019)
  • Author(s): Vu Minh Duc、柿内梨那、豊福肇、小尾岳士、中馬猛久
  • Organizer: 第９２回日本細菌学会
• [Presentation] Genetic characterization of beta-lactam resistance in Salmonella isolated from broiler chickens in Kagoshima, Japan (2019)
  • Author(s): Vu Minh Duc、柿内梨那、豊福肇、小尾岳士、中馬猛久
  • Organizer: 第１６２回日本獣医学会
• [Presentation] ブロイラー由来サルモネラにおける血清型の流行とβラクタム系抗菌剤耐性の消失 (2019)
  • Author(s): 宗安祥佳、柿内梨那、Vu Minh Duc、中馬猛久
  • Organizer: 第６８回日本獣医公衆衛生学会（九州）
• [Presentation] ブロイラー盲腸内容物由来サルモネラの陽性率、血清型、薬剤耐性状況の解析 (2019)
  • Author(s): ◯柿内梨那、下地康大、Vu Minh Duc、小尾岳士、中馬猛久
  • Organizer: 平成３０年度日本獣医師会獣医学術学会年次大会
• [Presentation] ブロイラーから分離されたサルモネラの血清型および薬剤耐性の状況 (2018)
  • Author(s): ◯柿内梨那、下地康大、Vu Minh Duc、小尾岳士、中馬猛久
  • Organizer: 第６７回日本獣医公衆衛生学会（九州）
• [Presentation] Emergence of Salmonella Schwarzengrund and antimicrobial susceptibility of Salmonella from broiler chicken in Kagoshima, Japan during 2013-2016 (2018)
  • Author(s): ◯Vu Minh Duc、豊福肇、小尾岳士、中馬猛久
  • Organizer: 第１６１回日本獣医学会