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Functional analysis of Tmem86a using oligodendorocyte progenitor cells

Research Project

Project/Area Number 18K06000
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 42020:Veterinary medical science-related
Research InstitutionOsaka Prefecture University

Principal Investigator

Komori Masayuki  大阪府立大学, 生命環境科学研究科, 教授 (40183347)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsプラスマローゲン / Tmem86a / リゾプラスマロゲナーゼ / オリゴデンドロサイト前駆細胞 / オリゴデンドロサイト / 膜タンパク質
Outline of Final Research Achievements

Since the experiments using oligodendrocyte progenitor cells was not successful, it was decided to perform functional analysis of Tmem86a using a heterologous expression system of FLAG-tagged human Tmem86a. Although heterogeneous expression of FLAG tagged human Tmem86a alone was not successful, four chimeric proteins (Ch1 to 4) fused so that the N-terminal side is Tmem86b and the C-terminal side is Tmem86a, were possible to express them in methylotrophic yeast. In particular, Ch4 includes a nearly full-length Tmem86a. In addition, the expression of TMEM86a mRNA was stronger in rat brains than in other tissues, suggesting that Tmem86a may play an important role in the brain.

Academic Significance and Societal Importance of the Research Achievements

FLAGタグ付加ヒトTmem86bを異種過剰発現する酵母ミクロソームから可溶化・精製した標品を用いてリポソーム再構成系でのリゾプラスマロゲナーゼ活性を測定したが検出できず、Tmem86bがリゾプラスマロゲナーゼを持たないというごく最近の報告(文献3)を支持する結果が得られた。一方、FLAGタグ付加ヒトTmem86bとTmem86aのキメラタンパク質のうち、ほぼ全長のTmem86aを含むCh4についてメタノール資化酵母を用いる異種発現系の確立に成功したことは、Tmem86aの生化学的な機能解析などを行う上で学術的に有用である。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (10 results)

All 2021 2019 2018 Other

All Int'l Joint Research (4 results) Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results)

  • [Int'l Joint Research] University of Groningen(オランダ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] University of Groningen(オランダ)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] University of Groningen(オランダ)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] University of Groningen(オランダ)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Carbon tetrachloride suppresses ER‐Golgi transport by inhibiting COPII vesicle formation on the ER membrane in the RLC‐16 hepatocyte cell line.2021

    • Author(s)
      Nakagawa H, Komori M, and Nishimura K.
    • Journal Title

      Cell Biol Int.

      Volume: 45 Pages: 633-641

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] ラット肝由来株化細胞において四塩化炭素は小胞体-Golgi間小胞輸送を阻害する2019

    • Author(s)
      中川博史、小森雅之、西村和彦
    • Organizer
      第46回日本毒性学会学術年会
    • Related Report
      2019 Research-status Report
  • [Presentation] ラット肝由来株化細胞を用いた四塩化炭素肝毒性での細胞内小胞輸送阻害メカニズムの解析2019

    • Author(s)
      中川博史、小森雅之、西村和彦
    • Organizer
      第162回日本獣医学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Heterologous expression of N-terminal FLAG-tagged human lysoplasmalogenase2018

    • Author(s)
      西川和雅、國澤卓磨、Ida J.van der Klei、小森雅之
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Visualization of import of peroxisomal matrix proteins in derived cells from H. polymorpha pex14 deletion strain2018

    • Author(s)
      矢野敦也、奥添彩加、国本将樹、Ida J.van der Klei、小森雅之
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Phospholipase D1またはPhospholipase D2の抑制はER-Golgi間小胞輸送調節を介してERストレス由来アポトーシスを誘導する2018

    • Author(s)
      中川博史、硲一樹、小森雅之、西村和彦
    • Organizer
      第161回日本獣医学会学術集会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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