| Project/Area Number |
18K06000
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42020:Veterinary medical science-related
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
Komori Masayuki 大阪府立大学, 生命環境科学研究科, 教授 (40183347)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | プラスマローゲン / Tmem86a / リゾプラスマロゲナーゼ / オリゴデンドロサイト前駆細胞 / オリゴデンドロサイト / 膜タンパク質 |
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| Outline of Final Research Achievements |
Since the experiments using oligodendrocyte progenitor cells was not successful, it was decided to perform functional analysis of Tmem86a using a heterologous expression system of FLAG-tagged human Tmem86a. Although heterogeneous expression of FLAG tagged human Tmem86a alone was not successful, four chimeric proteins (Ch1 to 4) fused so that the N-terminal side is Tmem86b and the C-terminal side is Tmem86a, were possible to express them in methylotrophic yeast. In particular, Ch4 includes a nearly full-length Tmem86a. In addition, the expression of TMEM86a mRNA was stronger in rat brains than in other tissues, suggesting that Tmem86a may play an important role in the brain.
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| Academic Significance and Societal Importance of the Research Achievements |
FLAGタグ付加ヒトTmem86bを異種過剰発現する酵母ミクロソームから可溶化・精製した標品を用いてリポソーム再構成系でのリゾプラスマロゲナーゼ活性を測定したが検出できず、Tmem86bがリゾプラスマロゲナーゼを持たないというごく最近の報告(文献3)を支持する結果が得られた。一方、FLAGタグ付加ヒトTmem86bとTmem86aのキメラタンパク質のうち、ほぼ全長のTmem86aを含むCh4についてメタノール資化酵母を用いる異種発現系の確立に成功したことは、Tmem86aの生化学的な機能解析などを行う上で学術的に有用である。
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