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Analysis of the mechanism of increase in antinuclear antibody levels due to differences in genetic background of mice

Research Project

Project/Area Number 18K06027
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 42040:Laboratory animal science-related
Research InstitutionUniversity of Yamanashi

Principal Investigator

ITO Sadahiro  山梨大学, 大学院総合研究部, 助教 (30345722)

Co-Investigator(Kenkyū-buntansha) 長友 啓明  山梨大学, 大学院総合研究部, 講師 (30746813)
神沼 修  広島大学, 原爆放射線医科学研究所, 教授 (80342921)
Project Period (FY) 2018-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsアレルギー / 免疫反応の系統差 / 皮膚疾患 / 肥満と突然死 / 自己免疫疾患 / 炎症の系統差 / 血清アミロイドP成分 / ゲノム編集 / miRNA / 転写因子 / 結合配列 / 抗核抗体価の上昇 / ゲノム編集技術 / マウスの遺伝子背景
Outline of Final Research Achievements

We initially investigated the 129/Sv//Ev region { The plan was to randomly destroy 20 genes related to immunity from D1Mit36 (76.73cM) to D1Mit115 (82.78cM) using genome editing. Therefore, we performed whole genome sequencing on one homozygous individual.
However, as we continued backcrossing to C57BL/6N and maintained the line, the antinuclear antibody titer no longer increased. Therefore, it is no longer possible to edit the genome using an increase in antinuclear antibody titer as an indicator.

Academic Significance and Societal Importance of the Research Achievements

ホモ個体において、免疫に関係する表現系が現れた。前脚と後脚の間の腹部を体液が出る程掻きむしる個体と肥満して突然死する個体が出てきた。
抗核抗体価の上昇に関わる領域は、129/Sv//Ev領域{D1Mit36(76.73cM)からD1Mit115(82.78cM)}の中ではなく、ごく近い近傍であることが強く示唆された。

Report

(7 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report

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Published: 2018-04-23   Modified: 2025-01-30  

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