| Project/Area Number |
18K06067
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 43010:Molecular biology-related
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | エピジェネティクス / DNAメチル化 / 神経幹細胞 |
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| Outline of Final Research Achievements |
Neural stem cells are cells that have self-renewal ability and have potential to differentiate into neurons, astrocytes, and oligodendrocytes that make up the brain and nervous system. However, neural stem cells do not have pluripotency in the early stage of development, and gradually acquire the ability to differentiate into the above-mentioned cell types as development progresses. In this research project, we focused on the epigenetic modification of DNA methylation and analyzed changes in the differentiation potential of neural stem cells. As a result, it was clarified that the methylation state dynamically fluctuates by three steps and the ability to differentiate into each cell type is acquired.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究課題では、発生段階に沿った神経幹細胞の性質変化における、DNAメチル化のゲノムワイドな変動が明らかとなった。また、神経幹細胞から各細胞系譜へと運命づけられる際のDNAメチル化の変動も明らかとなり、DNAのメチル化が実際に細胞の分化制御に関与している事が示された。
DNAメチル化は神経系だけでなく、ほとんどすべての細胞種で普遍的にみられる現象であることからも、そのメカニズム解明は他分野への波及効果も大きいと考えられる。
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