Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Outline of Final Research Achievements |
Mannose trimming of N-glycan regulates endoplasmic reticulum (ER)-associated degradation of misfolded glycoproteins. In this study, we found that EDEM2, a putative ER-localized mannosidase, was stably disulfide-bounded to TXNDC11, an ER-localized protein with five thioredoxin-like domains. This covalent association was essential for mannose trimming and glycoprotein degradation. We also clearly demonstrated for the first time that EDEM2-TXNDC11 complex purified from culture cells had mannosidase activity against N-glycan in vitro.
|