| Project/Area Number |
18K06135
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 43030:Functional biochemistry-related
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| Research Institution | Akita Prefectural University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 酵素学 / 構造生物学 / 澱粉 / グリコーゲン / 多糖代謝関連酵素 / 構造機能解析 |
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| Outline of Final Research Achievements |
In this study, we performed structural and biochemical analyses of cyanobacterial GS, BE, and GlgX. Specific activity, substrate specificity, product specificity, role of surface binding site (SBS), and mechanism controlling the length of branched chains formed by BE were clarified. In addition, the crystal structures of BE isoform 3 and GlgX isoform 2 were determined. Furthermore, our results confirmed that GS, BE, and GlgX did not form enzyme-enzyme interaction. We have obtained important information in order to elucidate the mechanism controlling the branched structure of starch, although all of the research purposes were not achieved.
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| Academic Significance and Societal Importance of the Research Achievements |
澱粉の分岐構造は、物性や食味に関係することがわかっている。澱粉を生合成する酵素を用いて澱粉の分岐構造を制御できれば、澱粉素材（バイオプラスチックなど）としての応用利用が期待できる。しかし、分岐構造を制御する仕組みはわかっていなかった。本研究では、分岐構造を制御する仕組みを解き明かすために重要な情報を複数得ることができた。本研究で得られた成果は、分岐構造を制御した澱粉の酵素合成などの応用へ向けた研究基盤となるものである。将来的に分岐構造を制御した澱粉の生産が可能となれば、食料自給率の向上や澱粉素材の生産などに貢献できると期待される。
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