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An application software development for quantitative biological image analysis

Research Project

Project/Area Number 18K06272
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44020:Developmental biology-related
Research InstitutionCenter for Novel Science Initatives, National Institutes of Natural Sciences

Principal Investigator

Kato Kagayaki  大学共同利用機関法人自然科学研究機構(新分野創成センター、アストロバイオロジーセンター、生命創成探究, 生命創成探究センター, 特任助教 (30391915)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords生物画像データ解析 / 生物画像解析 / 生物画像情報解析
Outline of Final Research Achievements

There is an urgent need to efficiently extract and measure biologically important features from observational images in multidimensional imaging and large-capacity biological research in recent years. Moreover, various image data are produced in the whole biological field due to the wide range of observation objects. To deal with this situation, we have developed software specializing in biological image data analysis. This software system is a foundation that enables simple expression and description of biological features in images as geometric expressions, and an application can be constructed by relatively small-scale programming. Using this system, we performed quantitative image analysis in a wide range of biological experiments, from intracellular organelles in cultured cell lines to analysis of free migration patterns in fish.

Academic Significance and Societal Importance of the Research Achievements

本研究は,生物学研究において産生される画像データについて,その観察技法の発展に伴う多次元化・高精細化と近年のデータ科学的方法論に基づく大量データの解析法の開発という要望に対応するべく,生物画像データ解析に特化したソフトウェア基盤であり,特殊な顕微鏡からビデオ撮影した動画に至るまでの多岐に渡る画像データを透過的に処理,定量的な解析までを行うことを可能とするものである.今後,本システムを適用することによって,広範な生物学実験における観察画像の定量的な解析を効率よく行う系を構築することが可能となる.

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (19 results)

All 2022 2021 2020 2019 2018

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 5 results) Presentation (8 results) Book (1 results)

  • [Journal Article] Live imaging of avian epiblast and anterior mesendoderm grafting reveals the complexity of cell dynamics during early brain development2022

    • Author(s)
      Yoshihi Koya、Kato Kagayaki、Iida Hideaki、Teramoto Machiko、Kawamura Akihito、Watanabe Yusaku、Nunome Mitsuo、Nakano Mikiharu、Matsuda Yoichi、Sato Yuki、Mizuno Hidenobu、Iwasato Takuji、Ishii Yasuo、Kondoh Hisato
    • Journal Title

      Development

      Volume: 149 Issue: 6

    • DOI

      10.1242/dev.199999

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Neuronal Circuits That Control Rhythmic Pectoral Fin Movements in Zebrafish2020

    • Author(s)
      Uemura Yuto、Kato Kagayaki、Kawakami Koichi、Kimura Yukiko、Oda Yoichi、Higashijima Shin-ichi
    • Journal Title

      The Journal of Neuroscience

      Volume: 40 Issue: 35 Pages: 6678-6690

    • DOI

      10.1523/jneurosci.1484-20.2020

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Marcksl1 modulates endothelial cell mechanoresponse to haemodynamic forces to control blood vessel shape and size2020

    • Author(s)
      Kondrychyn Igor、Kelly Douglas J.、Carretero N?ria Taberner、Nomori Akane、Kato Kagayaki、Chong Jeronica、Nakajima Hiroyuki、Okuda Satoru、Mochizuki Naoki、Phng Li-Kun
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 5476-5493

    • DOI

      10.1038/s41467-020-19308-5

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Polar recruitment of RLD by LAZY1-like protein during gravity signaling in root branch angle control2020

    • Author(s)
      Furutani Masahiko、Hirano Yoshinori、Nishimura Takeshi、Nakamura Moritaka、Taniguchi Masatoshi、Suzuki Kanako、Oshida Ryuichiro、Kondo Chiemi、Sun Song、Kato Kagayaki、Fukao Yoichiro、Hakoshima Toshio、Morita Miyo Terao
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 1-13

    • DOI

      10.1038/s41467-019-13729-7

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Endfoot regeneration restricts radial glial state and prevents translocation into the outer subventricular zone in early mammalian brain development2019

    • Author(s)
      Fujita Ikumi、Shitamukai Atsunori、Kusumoto Fumiya、Mase Shun、Suetsugu Taeko、Omori Ayaka、Kato Kagayaki、Abe Takaya、Shioi Go、Konno Daijiro、Matsuzaki Fumio
    • Journal Title

      Nature Cell Biology

      Volume: 22 Issue: 1 Pages: 26-37

    • DOI

      10.1038/s41556-019-0436-9

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] イメージングデータ解析よろず相談所.大量画像解析のための自動化手法・応用編2019

    • Author(s)
      加藤輝
    • Journal Title

      実験医学

      Volume: 37 Pages: 2346-2353

    • Related Report
      2019 Research-status Report
  • [Journal Article] イメージングデータ解析よろず相談所.顕微観察画像の定量的情報解析とその落とし穴2019

    • Author(s)
      小山宏史,加藤輝
    • Journal Title

      実験医学

      Volume: 37 Pages: 2772-2777

    • Related Report
      2019 Research-status Report
  • [Journal Article] Elasticity-based boosting of neuroepithelial nukleokinesis via indirect energy transfer from mother to daughter2018

    • Author(s)
      Tomoyasu Shinoda, Arata Nagasaka, Yasuhiro Inoue, Ryo Higuchi, Yoshiaki Minami, Kagayaki Kato, Makoto Suzuki, Takefumi Kondo, Takumi Kawaue, Kanako Saito, Naoto Ueno, Yugo Fukazaawa, Masaharu Nagayama, Takashi Miura, Taiji Adachi, Takaki Miyata
    • Journal Title

      PLoS Biology

      Volume: 16 Issue: 4 Pages: 2004426-2004426

    • DOI

      10.1371/journal.pbio.2004426

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Solo and Keratin Filaments Regulate Epithelial Tubule Morphology2018

    • Author(s)
      Ryosuke Nishimura, Kagayaki Kato, Sachiko Fujiwara, Kazumasa Ohashi, Kensaku Mizuno
    • Journal Title

      Cell Structure and Function

      Volume: 43 Issue: 1 Pages: 95-105

    • DOI

      10.1247/csf.18010

    • NAID

      130007383366

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] 生物画像処理・解析を加速するImageJマクロプログラミング2018

    • Author(s)
      加藤輝
    • Journal Title

      実験医学

      Volume: 36 Pages: 1513-1520

    • Related Report
      2018 Research-status Report
  • [Presentation] 鳥類胚のライブイメージングが明らかにした、各脳領域の新しい前駆体マップと、脳形成を開始させる前部中内胚葉の作用2019

    • Author(s)
      Hisato Kondoh, Koya Yoshihi, Hideaki Iida, Machiko Teramoto, Kagayaki Kato
    • Organizer
      第52回日本発生生物学会大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ニワトリ初期胚細胞の新しい標識法とライブイメージングによって明らかになった脳の発生の新しい機構2019

    • Author(s)
      近藤 寿人, 吉氷 康矢, 寺元 万智子, 飯田 英明, 加藤 輝
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Ground stateにおけるマウスES細胞の不均一性の同定とその制御機構の解明2019

    • Author(s)
      堀江 恭二, 渡邊 仁美, 西村 陽介, 渡邊 日佳流, 関 真秀, 清田 晃央, 加藤 輝, 若本 祐一, 瀬々 潤, 鈴木 穣, 山田 拓司, 近藤 玄, 吉田 純子
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] PML bodyによる遺伝子転写制御メカニズムの解析~AP-CLaP法を用いて~2018

    • Author(s)
      栗原 美寿々、三宝 千秋、加藤 輝、重信 秀治、宮成 悠介
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Ground stateにおけるマウスES細胞の不均一性の同定2018

    • Author(s)
      堀江 恭二、渡邊 仁美、西村 陽介、渡邊 日佳流、関 真秀、清田 晃央、加藤 輝、若本 祐一、鈴木 穣、山田 拓司、近藤 玄、吉田 純子
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Live imaging of node graft and host cells to establish its role in head development2018

    • Author(s)
      Koya Yoshihi, Kagayaki Kato, Hisato Kondoh
    • Organizer
      Joint Annual Meeting of 51st JSDB and 70th JSCB
    • Related Report
      2018 Research-status Report
  • [Presentation] PCP factors are differentially involved in polarity establishment of ciliary orientation and cell elongation in the mouse oviduct.2018

    • Author(s)
      Fumiko Usami, Dongbo Shi, Kagayaki Kato, Toshihiko Fujimori
    • Organizer
      Joint Annual Meeting of 51st JSDB and 70th JSCB
    • Related Report
      2018 Research-status Report
  • [Presentation] Elasticity-based boosting of neuroepithelial nucleokinesis via indirect energy transfer from mother to daughter2018

    • Author(s)
      Tomoyasu Shinoda, Arata Nagasaka, Yasuhiro Inoue, Ryo Higuchi, Yoshiaki Minami, Kagayaki Kato, Makoto Suzuki, Takefumi kondo, Takumi Kawaue, Kanako Saito, Naoto Ueno, Yugo Fukazawa, Masaharu Nagayama, Takashi Miura, Taiji Adachi, Takaki Miyata
    • Organizer
      Joint Annual Meeting of 51st JSDB and 70th JSCB
    • Related Report
      2018 Research-status Report
  • [Book] 達人に訊くバイオ画像取得と定量解析Q&A2021

    • Author(s)
      加藤 輝、小山 宏史
    • Total Pages
      220
    • Publisher
      羊土社
    • ISBN
      9784758122504
    • Related Report
      2021 Annual Research Report 2020 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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