Study of gene function and its expression for the dipause of annual killiifish

• Project/Area Number: 18K06327
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44050:Animal physiological chemistry, physiology and behavioral biology-related
• Research Institution: The University of Tokyo
• Principal Investigator: KUROKAWA Daisuke 東京大学, 大学院理学系研究科(理学部), 助教 (40342779)
• Project Period (FY): 2018-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 発生休止 / 一年魚 / 細胞分裂 / 休眠 / 細胞

Outline of Final Research Achievements

The diapause of vertebrate was analyzed by using annual killifish (Nothobranchius korthausae).
In order to investigate the fuctions of gene whose expression levels increase during diapause, we created mutants of these candidate gene by genome editing using CRISPR/Cas9.

Academic Significance and Societal Importance of the Research Achievements

これまでほとんど研究がされてこなかった脊椎動物の発生休止現象の理解をすすめるための基盤を作った。またこれらの実験を進める中で、比較的飼育の容易な東アフリカ産の一年魚Nothobranchius korthausaeの飼育技術を確立して系統を維持し、トランスジェニック魚の作成やゲノム編集法を確立した。

Research Products

• [Journal Article] Evolution and Developmental Diversity of Skin Spines in Pufferfishes (2019)
  • Author(s): Shono Takanori、Thiery Alexandre P.、Cooper Rory L.、Kurokawa Daisuke、Britz Ralf、Okabe Masataka、Fraser Gareth J.
  • Journal Title: iScience Volume: 19 Pages: 1248-1259
  • DOI: 10.1016/j.isci.2019.06.003
• [Presentation] 細胞・個体の寿命を研究するためのモデル動物「一年魚」 (2023)
  • Author(s): 黒川大輔
  • Organizer: 第70回 日本実験動物学会総会
  • Invited