Study of gene function and its expression for the dipause of annual killiifish

Research Project

Project/Area Number	18K06327
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 44050:Animal physiological chemistry, physiology and behavioral biology-related
Research Institution	The University of Tokyo
Principal Investigator	KUROKAWA Daisuke 東京大学, 大学院理学系研究科(理学部), 助教 (40342779)
Project Period (FY)	2018-04-01 – 2023-03-31
Project Status	Completed (Fiscal Year 2022)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords	発生休止 / 一年魚 / 細胞分裂 / 休眠 / 細胞
Outline of Final Research Achievements	The diapause of vertebrate was analyzed by using annual killifish (Nothobranchius korthausae). In order to investigate the fuctions of gene whose expression levels increase during diapause, we created mutants of these candidate gene by genome editing using CRISPR/Cas9.
Academic Significance and Societal Importance of the Research Achievements	これまでほとんど研究がされてこなかった脊椎動物の発生休止現象の理解をすすめるための基盤を作った。またこれらの実験を進める中で、比較的飼育の容易な東アフリカ産の一年魚Nothobranchius korthausaeの飼育技術を確立して系統を維持し、トランスジェニック魚の作成やゲノム編集法を確立した。