| Project/Area Number |
18K06509
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 46020:Anatomy and histopathology of nervous system-related
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
船戸 弘正 東邦大学, 医学部, 教授 (90363118)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | MPOA / 性的二型 / ISH / Moxd1 / 内側視索前野 / Creマウス / DREADD / AAV / 性行動 / 攻撃行動 / 神経解剖 / 本能行動 |
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| Outline of Final Research Achievements |
The medial preoptic area has been known to be a brain center of instinctive behaviors. We identified some gene expressions for the markers of sexually dimorphic area in the medial preoptic area, such as Moxd1 gene. To verify the functions of the sexually dimorphic cell groups, we investigated the behavioral change in the transgenic Cre-driver mice introduced some genes by AAV local injection. As a result, it was suggested that the cell groups which showed sexual dimorphism had functions on the sexual behavior and/or the parental behavior. In addition, we developed new ISH protocol. In this ISH protocol, we can visualize single copy mRNA in tissue, and other advantages in our protocol were shown.
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| Academic Significance and Societal Importance of the Research Achievements |
近年、性は社会的にも連続的なものであり多様性があるものとして捉えられるようになりつつあるものの、性典型的な行動がどのようなメカニズムで表出されるのかについては大部分が不明である。そのような性差を脳の構造的な差異として示すことが出来るという点に本研究の意義がある。また、新規ISH法は非常に簡便かつ低コストであり、様々な生体情報をこれまで以上に容易に得ることが出来る技術である。
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