Developmental change in synaptic plasticity of corticomotoneuronal direct connections: Do motoneurons learn?
| Project/Area Number |
18K06528
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 46030:Function of nervous system-related
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| Research Institution | Teikyo University |
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Principal Investigator |
Ohno Takae 帝京大学, 医学部, 准教授 (60508109)
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| Co-Investigator(Kenkyū-buntansha) |
福田 諭 帝京大学, 医学部, 助教 (50425641)
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| Project Period (FY) |
2018-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 運動ニューロン / 皮質運動ニューロン直接結合 / シナプス除去 / 光遺伝学的刺激法 / ホールセル記録 / 皮質運動ニューロン直接接合 / シナプス可塑性 / 長期増強(LTP) / GluN2B / 脊髄運動ニューロン / 皮質脊髄路 / 可塑性 / 急性スライス / 長期増強LTP / 脊髄 |
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| Outline of Final Research Achievements |
The developmental time course of the positive ratio and the synaptic strength of cortico-motoneuronal direct connections showed that the direct connections were formed by 1W after birth, reached a plateau at 2W, and then the elimination began and disappeared after 3W. The results were submitted to an English journal (Neuroscience, 2021).
This process of synapse removal was accompanied by the change in the pre-synaptic state due to a change in the paired pulse ratio, but there was no change in the amplitude of the miniture EPSCs, indicating that the post-synaptic state was not involved in the process. Selective knockout of GluN2B from motoneurons partially inhibited synaptic removal, suggesting that GluN2B is involved in this synaptic removal process.
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| Academic Significance and Societal Importance of the Research Achievements |
組織の低酸素化を最大限遅らせ、迅速に脊髄を取り出す独自の手法にて頸髄急性スライスを作成することで、世界的にも困難とされてきた生後３週以降の脊髄運動ニューロンからのホールセル記録を可能にしたことは、運動ニューロンの研究に大きく貢献できるものと考える。また、皮質脊髄路シナプス除去過程ではpresynapseに変化が生じているもpostの関与は明らかでなく、NMDA受容体サブユニットの１つであるGluN2Bを運動ニューロンから選択的にノックアウトすると、シナプス除去が部分的に阻害された事から、本過程にはGluN2Bが関与しており、遺伝的要因に加え、活動依存的な影響も受けていることが示唆された。
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Report
(6 results)
Research Products
(14 results)
