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Super resolution microscopy for long term observation of intravital nano-structures

Research Project

Project/Area Number 18K06591
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
Research InstitutionJuntendo University (2021)
Center for Novel Science Initatives, National Institutes of Natural Sciences (2019-2020)
Hokkaido University (2018)

Principal Investigator

Otomo Kohei  順天堂大学, 大学院医学研究科, 准教授 (40547204)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords超解像顕微鏡 / STED顕微鏡 / 二光子顕微鏡 / スピニングディスク / 分子配向イメージング / in vivoイメージング / 二光子顕微鏡法 / 多色イメージング / 線形アンミキシング / ナノシート / ライブイメージング / 補償光学 / ナノ薄膜 / 深層学習 / 超解像顕微鏡法 / 偏光イメージング / バイオイメージング
Outline of Final Research Achievements

Two-photon excitation laser scanning microscopy (TPLSM) is widely used as a powerful method for the visualization of the dynamical molecular and cellular phenomena in living specimens. TPLSM is primarily used because of its superior penetration depth and less-invasiveness in specimens owing to its near-infrared excitation laser wavelength in comparison to the wavelength of single-photon excitation-based systems. In this research project, the principal researcher developed novel TPLSM methodologies based on the expertise about super-resolution microscopy and high-speed imaging for long-term visualizations of intravital nano/micro-structures.

Academic Significance and Societal Importance of the Research Achievements

蛍光バイオイメージング法は、生命科学からのニーズに応える形で、日進月歩の技術革新を続けている。その一方で、時空間分解能などの極限的なイメージング指標に対する生体毒性のトレードオフがかねてから指摘されている。多くの観察結果に対する侵襲性の影響は無視されるか、その影響が危惧される実験結果は排除されることがほとんどである。そこで、本研究で実施したイメージング技術開発は、侵襲性の低さについても重きを置いた。その結果として提案した新規顕微鏡システムは、特に、投薬や治療の生体への効果の正しい評価が鍵となる医学・薬学研究におけるツールとしての実用化が期待できる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (69 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (26 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 20 results,  Open Access: 16 results) Presentation (37 results) (of which Int'l Joint Research: 13 results,  Invited: 18 results) Book (1 results) Remarks (3 results)

  • [Int'l Joint Research] KU Leuven(ベルギー)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Columbia University(米国)

    • Related Report
      2020 Research-status Report
  • [Journal Article] Low-invasive 5D visualization of mitotic progression by two-photon excitation spinning-disk confocal microscopy2022

    • Author(s)
      Kamada Takafumi、Otomo Kohei、Murata Takashi、Nakata Kaito、Hiruma Shota、Uehara Ryota、Hasebe Mitsuyasu、Nemoto Tomomi
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 1-10

    • DOI

      10.1038/s41598-021-04543-7

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 何ができる? どこで使える? 先端蛍光顕微鏡つかい分けガイド2022

    • Author(s)
      大友康平、野中茂樹、亀井保博
    • Volume
      40
    • Pages
      67
    • DOI

      10.18958/6941-00002-0000019-00

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 新規的な技術を用いた二光子顕微鏡による生体イメージングの展開2022

    • Author(s)
      根本知己, 大友康平, 山口和志, 高橋泰伽
    • Journal Title

      レーザー研究

      Volume: 50 Pages: 68-71

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatial and Temporal Resolution Improvements on 2-Photon Microscopy2022

    • Author(s)
      大友康平、山口和志、石井宏和、根本知己
    • Journal Title

      Seibutsu Butsuri

      Volume: 62 Issue: 2 Pages: 131-133

    • DOI

      10.2142/biophys.62.131

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Protocol for constructing an extensive cranial window utilizing a PEO-CYTOP nanosheet for in vivo wide-field imaging of the mouse brain2021

    • Author(s)
      Takahashi Taiga、Zhang Hong、Otomo Kohei、Okamura Yosuke、Nemoto Tomomi
    • Journal Title

      STAR Protocols

      Volume: 2 Issue: 2 Pages: 100542-100542

    • DOI

      10.1016/j.xpro.2021.100542

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Focusing new light on brain functions: multiphoton microscopy for deep and super-resolution imaging2021

    • Author(s)
      Ishii Hirokazu、Otomo Kohei、Takahashi Taiga、Yamaguchi Kazushi、Nemoto Tomomi
    • Journal Title

      Neuroscience Research

      Volume: - Pages: 00245-5

    • DOI

      10.1016/j.neures.2021.11.011

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 生命多光子顕微鏡による深部高解像・超解像イメージング2021

    • Author(s)
      大友康平, 髙橋泰伽, 山口和志, 石井宏和, 根本知己
    • Journal Title

      光学

      Volume: 50 Pages: 141-146

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 二光子顕微鏡法の機能向上と生理学応用2021

    • Author(s)
      大友康平、根本知己
    • Journal Title

      レーザー学会第557回研究報告

      Volume: RTM-21 Pages: 11-16

    • Related Report
      2021 Annual Research Report
  • [Journal Article] 二光子顕微鏡法の技術展開と生体イメージング応用2021

    • Author(s)
      大友康平、根本知己
    • Journal Title

      OPTRONICS

      Volume: 10 Pages: 99-103

    • Related Report
      2021 Annual Research Report
  • [Journal Article] 3DeeCellTracker, a deep learning-based pipeline for segmenting and tracking cells in 3D time lapse images2021

    • Author(s)
      Wen Chentao、Miura Takuya、Voleti Venkatakaushik、Yamaguchi Kazushi、Tsutsumi Motosuke、Yamamoto Kei、Otomo Kohei、Fujie Yukako、Teramoto Takayuki、Ishihara Takeshi、Aoki Kazuhiro、Nemoto Tomomi、Hillman Elizabeth MC、Kimura Koutarou D
    • Journal Title

      eLife

      Volume: 10

    • DOI

      10.7554/elife.59187

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High-peak-power 918-nm laser light source based two-photon spinning-disk microscopy for green fluorophores2020

    • Author(s)
      Otomo Kohei、Goto Ai、Yamanaka Yumi、Hori Takashi、Nakayama Hiroshi、Nemoto Tomomi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 529 Issue: 2 Pages: 238-242

    • DOI

      10.1016/j.bbrc.2020.05.213

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] In vivo two-photon microscopic observation and ablation in deeper brain regions realized by modifications of excitation beam diameter and immersion liquid2020

    • Author(s)
      Yamaguchi Kazushi、Kitamura Ryoji、Kawakami Ryosuke、Otomo Kohei、Nemoto Tomomi
    • Journal Title

      PLOS ONE

      Volume: 15 Issue: 8 Pages: e0237230-e0237230

    • DOI

      10.1371/journal.pone.0237230

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] PEO-CYTOP Fluoropolymer Nanosheets as a Novel Open-Skull Window for Imaging of the Living Mouse Brain2020

    • Author(s)
      Takahashi Taiga、Zhang Hong、Kawakami Ryosuke、Yarinome Kenji、Agetsuma Masakazu、Nabekura Junichi、Otomo Kohei、Okamura Yosuke、Nemoto Tomomi
    • Journal Title

      iScience

      Volume: 23 Issue: 10 Pages: 101579-101579

    • DOI

      10.1016/j.isci.2020.101579

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autophagy Contributes to the Quality Control of Leaf Mitochondria2020

    • Author(s)
      Nakamura Sakuya、Hagihara Shinya、Otomo Kohei、Ishida Hiroyuki、Hidema Jun、Nemoto Tomomi、Izumi Masanori
    • Journal Title

      Plant and Cell Physiology

      Volume: - Issue: 2 Pages: 229-247

    • DOI

      10.1093/pcp/pcaa162

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens2020

    • Author(s)
      Yamaguchi Kazushi、Otomo Kohei、Kozawa Yuichi、Tsutsumi Motosuke、Inose Tomoko、Hirai Kenji、Sato Shunichi、Nemoto Tomomi、Uji-i Hiroshi
    • Journal Title

      ACS Omega

      Volume: 6 Issue: 1 Pages: 438-447

    • DOI

      10.1021/acsomega.0c04888

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 新規パルス光源系を用いた二光子STED顕微鏡の開発2020

    • Author(s)
      石井宏和 , 大友康平 , Jui-Hung Hung , 堤元佐 , 横山弘之 , 根本知己
    • Journal Title

      光学

      Volume: 49 Pages: 232-232

    • Related Report
      2020 Research-status Report
  • [Journal Article] 二光子顕微鏡の技術開発と生物学応用2020

    • Author(s)
      大友康平、石井宏和、根本知己
    • Journal Title

      光アライアンス

      Volume: 31 Pages: 42-45

    • Related Report
      2020 Research-status Report
  • [Journal Article] Absorption, Fluorescence, and Two-Photon Excitation Ability of 5-Phenylisolidolo[2,1-a]quinolines2020

    • Author(s)
      Avena Ramon Francisco、Qiao Lin、Fujii Yuki、Otomo Kohei、Ishii Hirokazu、Suzuki Takeyuki、Tsujino Hirofumi、Uno Tadayuki、Tsutsumi Yasuo、Kawashima Yusuke、Takagi Tatsuya、Murai Kenichi、Nemoto Tomomi、Arisawa Mitsuhiro
    • Journal Title

      ACS Omega

      Volume: 5 Issue: 5 Pages: 2473-2479

    • DOI

      10.1021/acsomega.9b04070

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nanosheet wrapping-assisted coverslip-free imaging for looking deeper into a tissue at high resolution2020

    • Author(s)
      Zhang Hong、Yarinome Kenji、Kawakami Ryosuke、Otomo Kohei、Nemoto Tomomi、Okamura Yosuke
    • Journal Title

      PLOS ONE

      Volume: 15 Issue: 1 Pages: e0227650-e0227650

    • DOI

      10.1371/journal.pone.0227650

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Novel Katanin-Tethering Machinery Accelerates Cytokinesis2019

    • Author(s)
      Sasaki Takema、Tsutsumi Motosuke、Otomo Kohei、Murata Takashi、Yagi Noriyoshi、Nakamura Masayoshi、Nemoto Tomomi、Hasebe Mitsuyasu、Oda Yoshihisa
    • Journal Title

      Current Biology

      Volume: 29 Issue: 23 Pages: 4060-4070.e3

    • DOI

      10.1016/j.cub.2019.09.049

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two-photon STED nanoscopy realizing 100-nm spatial resolution utilizing high-peak-power sub-nanosecond 655-nm pulses2019

    • Author(s)
      Ishii Hirokazu、Otomo Kohei、Hung Jui-Hung、Tsutsumi Motosuke、Yokoyama Hiroyuki、Nemoto Tomomi
    • Journal Title

      Biomedical Optics Express

      Volume: 10 Issue: 7 Pages: 3104-3104

    • DOI

      10.1364/boe.10.003104

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics2019

    • Author(s)
      Inoue M, Takeuchi A, Manita S, Horigane S-i, Sakamoto M, Kawakami R, Yamaguchi K, Otomo K, Yokoyama H, Kim R, Yokoyama T, Takemoto-Kimura S, Abe M, Okamura M, Kondo Y, Quirin S, Ramakrishnan C, Imamura T, Sakimura K, Nemoto T, Kano M, Fujii H, Deisseroth K, Kitamura K, Bito H.
    • Journal Title

      Cell

      Volume: 177 Issue: 5 Pages: 1346

    • DOI

      10.1016/j.cell.2019.04.007

    • URL

      https://localhost/en/publications/a48843c1-543d-4414-a93c-3cab0e1df627

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 2光子励起顕微鏡法のナノイメージングへの展開2019

    • Author(s)
      石井宏和, 根本知己, 大友康平
    • Journal Title

      光アライアンス

      Volume: 30 Pages: 49-53

    • NAID

      40022056271

    • Related Report
      2019 Research-status Report
  • [Journal Article] 透過型液晶素子を用いた二光子励起STED顕微鏡2019

    • Author(s)
      大友康平, 石井宏和, 根本知己
    • Journal Title

      フォトニクスニュース

      Volume: 5 Pages: 192-195

    • Related Report
      2019 Research-status Report
  • [Journal Article] Real-Time Polarization-Resolved Imaging of Living Tissues Based on Two-Photon Excitation Spinning-Disk Confocal Microscopy2019

    • Author(s)
      Goto Ai、Otomo Kohei、Nemoto Tomomi
    • Journal Title

      Frontiers in Physics

      Volume: 7

    • DOI

      10.3389/fphy.2019.00056

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Differential contributions of nonmuscle myosin IIA and IIB to cytokinesis in human immortalized fibroblasts2019

    • Author(s)
      Yamamoto, K., Otomo, K., Nemoto, T., Ishihara, S., Haga, H., Nagasaki, A., Murakami, Y., Takahashi, M.
    • Journal Title

      Experimental Cell Research

      Volume: 376 Issue: 1 Pages: 67-76

    • DOI

      10.1016/j.yexcr.2019.01.020

    • NAID

      120006803950

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] 二光子顕微鏡法の高時空間分解能化と生体イメージング応用2022

    • Author(s)
      大友康平、根本知己
    • Organizer
      レーザー学会学術講演会第42回年次大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] High-resolution intravital imaging based on novel two-photon microscopic technologies2022

    • Author(s)
      Kohei OTOMO
    • Organizer
      The 10th RIEC International Symposium on Brain Functions and Brain Computer
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 二光子顕微鏡の高分解能化による生体内微細構造イメージング2022

    • Author(s)
      大友康平、石井宏和、根本知己
    • Organizer
      第95回日本薬理学会年会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 新規二光子顕微技術による低侵襲生体イメージング2021

    • Author(s)
      大友康平、根本知己
    • Organizer
      生理学研究所研究会 「細胞システム理解のためのシグナル応答原理解明の最前線」
    • Related Report
      2021 Annual Research Report
  • [Presentation] 二光子励起顕微鏡法の時空間分解能向上2021

    • Author(s)
      大友康平、石井宏和、根本知己
    • Organizer
      第11回 名古屋大学医・生理研合同シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] 二光子顕微鏡法の機能向上と生理学応用2021

    • Author(s)
      大友康平、根本知己
    • Organizer
      レーザー学会第557回研究会「光・レーザーの医学・医療応用」
    • Related Report
      2021 Annual Research Report
  • [Presentation] ヒメミカヅキモにおける蛍光標識CenH3タンパク質の発現による染色体可視化2021

    • Author(s)
      渡辺実咲, 村田隆, 堤元佐, 大友康平, 根本知己, 西山智明, 土金勇樹, 関本弘之
    • Organizer
      日本藻類学会第45回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 空間光位相変調器を用いた収差補正による生体組織深部の微細構造の可視化2021

    • Author(s)
      山口和志, 大友康平, 小澤祐市, 堤元佐, 猪瀬朋子, 平井健二, 佐藤俊一, 根本知己, 雲林院宏
    • Organizer
      日本化学会 第101春季年会
    • Related Report
      2020 Research-status Report
  • [Presentation] Two-Photon Excitation Spinning-Disk Confocal Microscopy and Its Biological Applications2020

    • Author(s)
      Kohei Otomo, Takafumi Kamada, Ai Goto, Yumi Yamanaka, Tomomi Nemoto
    • Organizer
      Focus On Microscopy 2020
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] 二光子励起顕微鏡の機能向上による多次元生細胞イメージング2020

    • Author(s)
      大友康平, 根本知己
    • Organizer
      生理学研究所研究会 「細胞システム理解のためのシグナル応答原理解明の最前線」
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Two-photon excitation confocal spinning-disk microscopy for real-time visualizations of biological phenomena2020

    • Author(s)
      Kohei Otomo
    • Organizer
      A3 Foresight & 5 Star Alliance Joint Workshop on Organic/Inorganic Hybrid Nano Materials and Bio Imaging
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Improving two-photon excitation microscopy for deeper, sharper and/or faster intravital imaging2020

    • Author(s)
      Kohei Otomo
    • Organizer
      44th Meeting of Australian Society for Biophysics
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Origin of mitotic spindle microtubules in plant cells2020

    • Author(s)
      Takashi MURATA, Kohei OTOMO, Tomomi NEMOTO, Mitsuyasu HASEBE
    • Organizer
      第 58 回 日本生物物理学会年会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Measurement of physical properties underlying the chromatin mobility in interphase nuclei2020

    • Author(s)
      Noritaka MOTOKI, Kohei OTOMO, Tomomi NEMOTO, Akatsuki KIMURA
    • Organizer
      第 58 回 日本生物物理学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] Improvements of two-photon microscopy techniques for understanding intravital phenomena2020

    • Author(s)
      大友康平, 根本知己
    • Organizer
      第8回 脳機能と脳型計算機に関するRIEC国際シンポジウム
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 生命現象の可視化のための二光子顕微技術開発2019

    • Author(s)
      大友康平
    • Organizer
      日本分光学会 令和元年度 生細胞分光部会シンポジウム
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] レーザー走査型蛍光顕微鏡の基礎, 光技術応用による機能向上2019

    • Author(s)
      大友康平
    • Organizer
      定量生物学の会 北海道キャラバン
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 生物イメージングのための多光子顕微技術開発2019

    • Author(s)
      大友康平、日比輝正、小澤祐市、横山弘之、佐藤俊一、根本知己
    • Organizer
      第7回糸状菌分子生物学研究会若手の会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Improvements of two-photon excitation fluorescence microscopy for deeper, sharper and/or faster bio imaging2019

    • Author(s)
      Kohei Otomo
    • Organizer
      JSPS Core-to Core Program A iC-Cubix3 Kick-off meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 二光子励起顕微鏡の機能向上と生体内部高精細イメージング2019

    • Author(s)
      大友康平
    • Organizer
      第14回 日本分子イメージング学会学術集会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Improving two-photon microscopy for deeper, sharper and/or faster bioimaging2019

    • Author(s)
      Kohei Otomo
    • Organizer
      NIG seminar, the 2050th Biological Symposium
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 2光子スピニングディスク顕微鏡による低侵襲4Dスペクトルイメージング2019

    • Author(s)
      鎌田恭史、大友康平、村田隆、比留間翔太、上原亮太、長谷部光泰、根本知己
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] In vivo visualization of dendritic spines in deep regions of the mouse frontal cortex with two-photon excitation adaptive optical (AO) microscopy2019

    • Author(s)
      Kazushi Yamaguchi, Kohei Otomo, Yuichi Kozawa, Shunichi Sato and Tomomi Nemoto
    • Organizer
      定量生物学の会 北海道キャラバン
    • Related Report
      2019 Research-status Report
  • [Presentation] Four-color three-dimensional live cell imaging by two-photon excitation spinning disk confocal microscopy2019

    • Author(s)
      Kohei Otomo, Takafumi Kamada, Takashi Murata, Mitsuyasu Hasebe, Tomomi Nemoto
    • Organizer
      Focus On Microscopy 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Compact two-photon excitation STED nanoscopy with high-peak-power sub-nanosecond 655-nm pulsed light source2019

    • Author(s)
      Hirokazu Ishii, Kohei Otomo, Jui-Hung Hung, Motosuke Tsutsumi, Hiroyuki Yokoyama, Tomomi Nemoto
    • Organizer
      Focus On Microscopy 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Real-TIme Polarization-Resolved Imaging of Living Mice Tissues by Two-Photon Excitation Spinning Disk Confocal Microscopy2019

    • Author(s)
      K. Otomo, A. Goto, T. Nemoto
    • Organizer
      The 66th NIBB Conference / ABiS International Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Improvements of two-photon microscopy for intravital micro/nano-imaging2019

    • Author(s)
      K. Otomo, T. Nemoto
    • Organizer
      NIC@HU seminar "Technological developments of laser scanning fluorescence microscopy"
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Improving two-photon microscopy for clear visualization of sub cellular structures2018

    • Author(s)
      K. Otomo, T. Nemoto
    • Organizer
      Nikon Imaging Center Directors' meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Improving two-photon microscopy for clear visualization of intravital structures2018

    • Author(s)
      K. Otomo, T. Nemoto
    • Organizer
      The 1st international joint symposium of CEFMS-NTCU, RCAS-AS and 5-star Alliance
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 多点走査型二光子顕微鏡による四重標識生細胞の三次元経時観察2018

    • Author(s)
      鎌田恭史, 大友康平, 村田隆, 長谷部光泰, 根本知己
    • Organizer
      第27回 バイオイメージング学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 多点走査型二光子顕微鏡への偏光分離光学系の導入と生体イメージングへの応用2018

    • Author(s)
      後藤亜衣, 大友康平, 根本知己
    • Organizer
      第27回 バイオイメージング学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 高速偏光分解イメージング法による生体組織の観察2018

    • Author(s)
      後藤亜衣, 大友康平, 根本知己
    • Organizer
      第98回 日本生理学会 北海道地方会
    • Related Report
      2018 Research-status Report
  • [Presentation] 多点走査型二光子顕微鏡への偏光分離光学系の導入と生体組織の分子配光イメージング2018

    • Author(s)
      後藤亜衣, 大友康平, 根本知己
    • Organizer
      第41回 日本分子生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 多点走査型二光子顕微鏡を用いたマウス生体脳のin vivoイメージング2018

    • Author(s)
      安宅光倫, 鎌田恭史, 大友康平, 根本知己
    • Organizer
      第41回 日本分子生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Two-photon excitation spinning disk confocal microscopy utilizing two laser light sources2018

    • Author(s)
      T. Kamada, K. Otomo, T. Murata, M. Hasebe, T. Nemoto
    • Organizer
      The 19th RIES-Hokudai international symposium
    • Related Report
      2018 Research-status Report
  • [Presentation] High-Speed Polarization-Resolved Imaging of Living Mice Tissues by Two-Photon Excitation Multi-Point Scanning Microscopy2018

    • Author(s)
      A. Goto, K. Otomo, T. Nemoto
    • Organizer
      The 19th RIES-Hokudai international symposium
    • Related Report
      2018 Research-status Report
  • [Presentation] 二光子スピニングディスク共焦点顕微鏡の開発と多次元イメージングへの展開2018

    • Author(s)
      大友康平
    • Organizer
      多次元性細胞イメージング研究会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Book] イメージングの選び方・使い方100+ (編:原田 慶恵、永井 健治)2018

    • Author(s)
      大友康平、根本知己
    • Total Pages
      236
    • Publisher
      羊土社
    • ISBN
      9784758103756
    • Related Report
      2018 Research-status Report
  • [Remarks] Researchmap

    • URL

      https://researchmap.jp/kotomo1109

    • Related Report
      2021 Annual Research Report
  • [Remarks] 生命創成探究センター バイオフォトニクス研究グループ ホームページ

    • URL

      https://www.nips.ac.jp/bp/index.html

    • Related Report
      2021 Annual Research Report
  • [Remarks] 順天堂大学 大学院医学研究科 生化学・生体システム医科学 ホームページ

    • URL

      http://www.dbsb.science/

    • Related Report
      2021 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

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