Comprehensive study on diversity of Curcuma drugs used as medicines / food supplements for standardization, responding to a global society
| Project/Area Number |
18K06714
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 47050:Environmental and natural pharmaceutical resources-related
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| Research Institution | University of Toyama |
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Principal Investigator |
Komatsu Katsuko 富山大学, 学術研究部薬学・和漢系, 教授 (50225570)
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| Co-Investigator(Kenkyū-buntansha) |
當銘 一文 富山大学, 学術研究部薬学・和漢系, 准教授 (80563981)
朱 シュウ 富山大学, 学術研究部薬学・和漢系, 助教 (20377360)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ウコン属生薬 / クルクミノイド生合成酵素遺伝子 / ジケタイドCoA生合成酵素遺伝子 / trnK遺伝子 / 精油成分 / Curcuma / 標準化 / 遺伝子多型 / 抗炎症作用 |
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| Outline of Final Research Achievements |
Curcuma-related drugs and food supplements have been used worldwide. However, correct identification and quality assessment have not been conducted, because classification of some species is debated and nomenclature is inconsistent among countries. To elucidate molecular markers of Curcuma species in Asia and to solve the confusion on the botanical origin of crude drugs, molecular analysis based on the intron length polymorphism (ILP) in genes encoding diketide-CoA synthase and curcumin synthase and the trnK sequences was performed using plant specimens and crude drugs from 9 countries. Each specimen showed species-specific ILP patterns, and based on the ILP markers besides trnK sequences, the botanical origins of crude drugs were determined. Moreover, GC-MS analysis of the genetically-authenticated Curcuma rhizomes and crude drugs revealed their species-specific essential oil composition. By applying above three methods international standardization of Curcuma drugs could be expected.
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| Academic Significance and Societal Importance of the Research Achievements |
ウコン属生薬は生薬配合製剤、保健機能食品などの素材として広く利用されているが、その資源は日本を含めたアジア各国の栽培種に依存している。同属植物は分類学上の混乱があり、それが生薬にも波及しているため、基原植物の学名が不確かで、成分的にも安定性を欠くものが流通している。本研究において、クルクミノイド生合成に関与する酵素遺伝子のイントロン長多型、葉緑体trnK遺伝子配列の解析と精油成分の分析を行い、各生薬を標準化するための指標を示した。これら3方法を行うことにより、同属植物の分類並びに国際的な生薬の学名の整理に繋がることが期待される。また、品質が担保された生薬の供給にも繋がる。
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Report
(4 results)
Research Products
(6 results)
