Analysis of endogenous immune adjuvant in Japanese cedar pollen

• Project/Area Number: 18K06723
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47050:Environmental and natural pharmaceutical resources-related
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: Adachi Yoshiyuki 東京薬科大学, 薬学部, 准教授 (60222634)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: スギ花粉症 / （１→３）-β-D-グルカン / デクチン-1 / 自然免疫 / アレルギー / スギ花粉 / 自然免疫応答 / (１→３)-β-D-グルカン / Dectin-1 / 糖鎖 / 樹状細胞 / レクチン様受容体

Outline of Final Research Achievements

The applicant showed the potential of (1→3)-β-D-glucan in cedar pollen particles and analyzed the pollen BG content and structural characteristics. Unlike fungal cell wall BGs, the BGs in pollen had only a few (1→6)-branched structures, suggesting the presence of linear (1→3)-β-D-glucan. Pollen β-glucan binds to innate immune system receptor proteins and also acts on mammalian Dectin-1 to activate dendritic cells and Dectin-1-transduced cell lines. This Dectin-1 reactivity was also found to be involved in the production of allergen-specific IgE, the cause of pollinosis.

Academic Significance and Societal Importance of the Research Achievements

スギ花粉症は、我が国において国民病とも言われるほど有病率の高い免疫疾患である。本研究では、スギ花粉に含まれる糖鎖成分が糖鎖受容体Dectin-1に作用して花粉症を発症させることを示した。この発見は、花粉の自然免疫活性化物質を明確にした点で学術的な独自性が高いものと考えられる。花粉糖鎖とその受容体との相互作用をコントロールする新たな花粉症治療法開発への展開も期待され、社会的意義も大きいと考えられる。

Research Products

• [Journal Article] Binding Specificity of a New Artificial β-Glucan Recognition Protein and Its Application to β-Glucan Detection in Mushroom Extracts (2021)
  • Author(s): Adachi Yoshiyuki、Kanno Takashi、Ishibashi Ken-ichi、Yamanaka Daisuke、Motoi Akitomo、Motoi Masuro、Ohno Naohito
  • Journal Title: International Journal of Medicinal Mushrooms Volume: 23 Issue: 4 Pages: 1-12
  • DOI: 10.1615/intjmedmushrooms.2021038074
  • Peer Reviewed
• [Journal Article] Possibility of Japanese Cedar Pollen Causing False Positives in the Deep Mycosis Test (2021)
  • Author(s): Kanno Takashi、Kim Changmin、Yamanaka Daisuke、Ishibashi Ken-ichi、Tanaka Hiroshi、Ohno Naohito、Adachi Yoshiyuki
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 4 Pages: 2135-2135
  • DOI: 10.3390/ijms22042135
  • Peer Reviewed / Open Access
• [Journal Article] Latent 1,3-β-D-glucan acts as an adjuvant for allergen-specific IgE production induced by Japanese cedar pollen exposure (2021)
  • Author(s): Kanno Takashi、Adachi Yoshiyuki、Ohashi-Doi Katsuyo、Matsuhara Hiroki、Hiratsuka Rie、Ishibashi Ken-ichi、Yamanaka Daisuke、Ohno Naohito
  • Journal Title: Allergology International Volume: 70 Issue: 1 Pages: 105-113
  • DOI: 10.1016/j.alit.2020.08.004
  • NAID: 130007966804
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access
• [Journal Article] スギ花粉内在性β-グルカンによる樹状細胞活性化と花粉アレルゲン特異的抗体産生の促進作用 (2021)
  • Author(s): 安達 禎之・菅野 峻史・平塚 理恵・大野 尚仁
  • Journal Title: アレルギーの臨床 Volume: 41 Pages: 75-79
• [Journal Article] スギ花粉に内在するβ-グルカンの 自然免疫活性化作用と抗体産生促進作用 (2020)
  • Author(s): 安達禎之、菅野峻史、平塚理恵、大野尚仁
  • Journal Title: アレルギーの臨床 Volume: 40 Pages: 53-57
• [Journal Article] N-Terminal (1→3)-β-d-Glucan Recognition Proteins from Insects Recognize the Difference in Ultra-Structures of (1→3)-β-d-Glucan (2019)
  • Author(s): Adachi Yoshiyuki、Ishii Masaki、Kanno Takashi、Tetsui Junko、Ishibashi Ken-ichi、Yamanaka Daisuke、Miura Noriko、Ohno Naohito
  • Journal Title: International Journal of Molecular Sciences Volume: 20 (14) Issue: 14 Pages: 3498-3498
  • DOI: 10.3390/ijms20143498
  • Peer Reviewed / Open Access
• [Presentation] スギ花粉によるリムルスGテストへの影響の検討 (2021)
  • Author(s): 菅野峻史、山中大輔、安達禎之
  • Organizer: 日本薬学会第141年会
• [Presentation] 1,3-β-glucan in the Japanese ceder pollen activates innate immune response via dectin-1 (2020)
  • Author(s): Y. Adachi, T. Kanno, K. Ohashi-doi, H. Matsuhara, R. Hiratsuka, K.Ishibashi, D. Yamanaka, N. Ohno
  • Organizer: EAACI Annual Congress 2020
  • Int'l Joint Research
• [Presentation] Latent 1,3-β-D-glucan in Japanese cedar pollen facilitated allergen-specific IgE production (2020)
  • Author(s): Takashi Kanno, Yoshiyuki Adachi, Katsuyo Ohashi-Doi, Hiroki Matsuhara, Rie Hiratsuka, Yoichiro Iwakura, Daisuke Yamanaka, Ken-Ichi Ishibashi, Naohito Ohno
  • Organizer: EAACI Annual Congress 2020
  • Int'l Joint Research
• [Presentation] Latent 1,3-β-D-glucan acts as an adjuvant for allergen-specific IgE production induced by Japanese cedar pollen exposure (2020)
  • Author(s): Takashi Kanno, Yoshiyuki Adachi, Katsuyo Ohashi-Doi, Hiroki Matsuhara, Rie Hiratsuka, Ken-ichi Ishibashi, Daisuke Yamanaka, and Naohito Ohno
  • Organizer: JSA/WAO XXVII World Allergy Congress
  • Int'l Joint Research
• [Presentation] スギ花粉によるDectin-1を介した樹状細胞成熟化への影響の検討 (2020)
  • Author(s): 金 チャンミン，菅野 峻史，安達 禎之，山中 大輔，石橋 健一，岩倉 洋一郎，大野 尚仁
  • Organizer: 日本薬学会 第140年会
• [Presentation] スギ花粉中のβ-1,3-glucan局在と自然免疫賦活化作用 (2019)
  • Author(s): 菅野 峻史，安達 禎之，平塚 理恵，岩倉 洋一郎，大野 尚仁
  • Organizer: 第68回日本アレルギー学会学術大会
• [Presentation] スギ花粉中1,3-β-glucanによる抗原特異的免疫グロブリン産生誘導作用 (2019)
  • Author(s): 菅野 峻史，安達 禎之，平塚 理恵，岩倉 洋一郎，大野 尚仁
  • Organizer: 第30回日本生体防御学会学術総会
• [Presentation] (1→3)-β-グルカンの免疫賦活作用におけるデクチンー１の影響 (2019)
  • Author(s): 安達 禎之
  • Organizer: 第92回日本生化学学会大会
• [Presentation] スギ花粉中のβ-1,3-glucan局在と自然免疫賦活化作用 (2019)
  • Author(s): 菅野峻史、安達禎之、平塚理恵、岩倉洋一郎、大野尚仁
  • Organizer: 第68回日本アレルギー学会学術大会
• [Patent(Industrial Property Rights)] β-1,3-1,6-グルカンの測定方法 (2019)
  • Inventor(s): 中田秀孝, 安達禎之, 石橋健一, 山中大輔, 大野尚仁
  • Industrial Property Rights Holder: 中田秀孝, 安達禎之, 石橋健一, 山中大輔, 大野尚仁
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2019-209679
  • Filing Date: 2019
• [Patent(Industrial Property Rights)] β-グルカン結合タンパク質、β-グルカン検出キット、人工DNAおよび細菌 (2019)
  • Inventor(s): 安達禎之 , 大野尚仁 , 元井益郎 , 元井章智
  • Industrial Property Rights Holder: 安達禎之 , 大野尚仁 , 元井益郎 , 元井章智
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2019-107248
  • Filing Date: 2019