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Exploration and functional analysis of novel RNA-interfering nucleic acids.

Research Project

Project/Area Number 18K07048
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 49030:Experimental pathology-related
Research InstitutionTokyo Medical University

Principal Investigator

Ohno Shin-ichiro  東京医科大学, 医学部, 講師 (90513680)

Co-Investigator(Kenkyū-buntansha) 熊谷 勝義  東京医科大学, 医学部, 助教 (20567911)
黒田 雅彦  東京医科大学, 医学部, 主任教授 (80251304)
原田 裕一郎  東京医科大学, 医学部, 助手 (80570168)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsRNA interference / microRNA / Non-coding RNA / RNA干渉 / miRNA / 核酸医薬 / ヘアピンRNA / ノンコーディングRNA
Outline of Final Research Achievements

Applicants discovered that RNA with a short hairpin structure (Guide hairpin RNA: ghRNA) has RNA interference activity from past research and development of nucleic acid drugs. As a result, it was clarified that some of the non-coding RNAs that are highly expressed in cells such as transfer RNA and small nuclear RNA are disrupted and have a ghRNA-like structure. In addition, the ghRNA was stably present in the cell by some mechanism and suppressed the expression of mRNA having a complementary sequence. This is considered to be a novel RNA-interfering nucleic acid that has undergone a production process different from that of the widely known microRNA.

Academic Significance and Societal Importance of the Research Achievements

microRNAによるRNA干渉核酸は、発生・分化および各種疾病の発症などに関与する重要な機構であることがよく知られている。本研究で発見された内在性ghRNAによるRNA干渉も各種の生命現象に関与している可能性が高く、分子生物学領域に与える影響は大きいと考えられる。また、何らかの疾病の発症に関与することも考えられ、今後の研究の発展が期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (10 results)

All 2020 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] 多様な核内マイクロRNAの機能2019

    • Author(s)
      大野慎一郎、黒田雅彦
    • Journal Title

      医学のあゆみ

      Volume: 269 (5) Pages: 337-341

    • Related Report
      2019 Research-status Report
  • [Journal Article] rAAV6-mediated miR-29b delivery suppresses renal fibrosis.2019

    • Author(s)
      Saito S, Ohno SI, Harada Y, Oikawa K, Fujita K, Mineo S, Gondo A, Kanno Y, KurodaM.
    • Journal Title

      Clin Exp Nephrol.

      Volume: 23 (12) Issue: 12 Pages: 1345-1356

    • DOI

      10.1007/s10157-019-01783-w

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] miR-34-AGO-TNRC6A complex transcriptionally up-regulates BLU tumor suppressor via binding of a novel promoter-associated lncRNA in lung cancer2020

    • Author(s)
      Shin-ichiro Ohno, Keiki Oikawa, Yuichirou Harada, Masahiko Kuroda
    • Organizer
      AACR Annual Meeting 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] miR-34-AGO complex transcriptionally up-regulate BLU tumor suppressor via binding of novel lncRNA2020

    • Author(s)
      大野慎一郎、老川桂生、原田裕一郞、黒田雅彦
    • Organizer
      第109回日本病理学会総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Development of novel nucleic acid drug targeting KRAS to suppress lung cancer2020

    • Author(s)
      Shin-ichiro Ohno, Yuichirou Harada, Masahiko Kuroda
    • Organizer
      第79回日本癌学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] miR-34 familyによる3p21.3領域に存在するがん抑制遺伝子の発現誘導2019

    • Author(s)
      大野慎一郎、老川桂生、原田裕一郎、黒田雅彦
    • Organizer
      第108回日本病理学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] The novel lncRNA expressed from the promoter of tumor suppressor BLU induces BLU expression via the miR-34-AGO complex.2019

    • Author(s)
      大野慎一郎、老川桂生、原田裕一郎、黒田雅彦
    • Organizer
      第78回日本癌学会学術総会
    • Related Report
      2019 Research-status Report
  • [Presentation] miR-34によるがん抑制遺伝子BLUの発現誘導2018

    • Author(s)
      大野慎一郎、老川桂生、原田裕一郎、黒田雅彦
    • Organizer
      第107回日本病理学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] Up-regulation pf BLU tumor suppressor by miR-34a2018

    • Author(s)
      大野慎一郎、三部 芳裕、弓野 梨紗、老川桂生、原田裕一郎、黒田雅彦
    • Organizer
      第10回日本RNAi研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] miR-34によるがん抑制遺伝子BLUの発現誘導2018

    • Author(s)
      大野慎一郎、老川桂生、原田裕一郎、黒田雅彦
    • Organizer
      第77回日本癌学会総会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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