Development of an optimal diagnostic system for parasitic diseases

• Project/Area Number: 18K07090
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 49040:Parasitology-related
• Research Institution: University of Miyazaki
• Principal Investigator: Maruyama Haruhiko 宮崎大学, 医学部, 教授 (90229625)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 寄生虫症 / 症候 / 鑑別診断 / 好酸球増多 / 抗体検査 / 寄生虫 / 診断 / 感度 / 特異度 / スクリーニング検査 / 確定診断 / スクリーニング / 臨床研究 / 組換え抗原 / 検査 / 的中率

Outline of Final Research Achievements

We analyzed clinical information and test results at our laboratory of 3,085 patients, who had been suspected as having parasitic infections. The most frequent symptom was pulmonary complaints and/or chest abnormal imaging, followed by abdominal symptoms, neurological disorder, and visual disorders. Peripheral blood eosinophilia or local eosinophil infiltration were noted in 54.5% of patients. Based on our laboratory tests, including measuring anti-parasite antibodies, infection-positive ratio was most high in creeping eruption (70.2%), followed by subcutaneous tumor (65.4%), and abnormal hepatic imaging (65.4%). When eosinophilia was accompanied with these symptoms, positive ratios were higher by approx. 10%.

Academic Significance and Societal Importance of the Research Achievements

線虫や条虫、吸虫による寄生虫症は疾患としては単純であり、正しく診断がつけばほとんどの場合に治療は容易である。ところがごく稀にしか遭遇しないため、一般臨床医にとっては典型的な苦手感染症になっている。本研究は、現在の日本の日常診療の中で、どのような訴えや所見がある場合にどのような寄生虫症の確率が高いのかを明らかにした。また同時に、虫体由来抗原または組換え寄生虫抗原を用いた信頼性の高い系を確立した。これらを組み合わせることにより、誰がどの医療機関を受診しても、正しく寄生虫症の診断に到達するような診断プロトコルの策定が可能となった。

Research Products

• [Int'l Joint Research] KEMRI(ケニア)
• [Int'l Joint Research] University of Medicine, 1(ミャンマー)
• [Int'l Joint Research] Vietnam Acad Sci Tech (VAST)(ベトナム)
• [Int'l Joint Research] University of Medicine 1/University of Medicine 2(ミャンマー)
• [Int'l Joint Research] Universidad Peruana Cayetano Heredia(ペルー)
• [Journal Article] Primaquine plus clindamycin as a promising salvage therapy for Pneumocystis jirovecii pneumonia: A retrospective analysis in Japanese patients (2021)
  • Author(s): Koga Michiko、Suganuma Akihiko、Kikuchi Tadashi、Yoshimura Yukihiro、Shoji Kensuke、Kobayashi Ichiro、Takezaki Shunichiro、Kato Yasuyuki、Kimura Mikio、Maruyama Haruhiko
  • Journal Title: Journal of Infection and Chemotherapy Volume: 27 Issue: 6 Pages: 924-928
  • DOI: 10.1016/j.jiac.2021.03.001
  • Peer Reviewed / Open Access
• [Journal Article] Enzyme-linked immunosorbent assay (ELISA) using recombinant Fasciola cathepsin L1 for the diagnosis of human fasciolosis caused by Fasciola hepatica/gigantica hybrid type. (2021)
  • Author(s): Sugiyama T, Ichikawa-Seki M, Sato H, Kounosu A, Tanaka M, Maruyama H
  • Journal Title: Parasitology International Volume: 82 Pages: 102311-102311
  • DOI: 10.1016/j.parint.2021.102311
  • Peer Reviewed / Open Access
• [Journal Article] Potential of antibody test using Schistosoma mansoni recombinant serpin and RP26 to detect light-intensity infections in endemic areas. (2021)
  • Author(s): Tanaka M, Kildemoes AO, Chadeka EA, Cheruiyot BN, Sassa M, Moriyasu T, Nakamura R, Kikuchi M, Fujii Y, de Dood CJ, Corstjens PLAM, Kaneko S, Maruyama H, Njenga SM, de Vrueh R, Hokke CH, Hamano S
  • Journal Title: Parasitology International Volume: 83 Pages: 102346-102346
  • DOI: 10.1016/j.parint.2021.102346
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Seroprevalence of toxoplasmosis among reproductive-aged women in Myanmar and evaluation of luciferase immunoprecipitation system assay (2020)
  • Author(s): Aye Khin Myo、Nagayasu Eiji、Nyunt Myat Htut、Zaw Ni Ni、Thant Kyaw Zin、Kyaw Myat Phone、Maruyama Haruhiko
  • Journal Title: BMC Infectious Diseases Volume: 20 Issue: 1
  • DOI: 10.1186/s12879-020-05650-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Meningitis patients with Angiostrongylus cantonensis may present without eosinophilia in the cerebrospinal fluid in northern Vietnam (2020)
  • Author(s): Hiraoka Tomoko、Cuong Ngo Chi、Hamaguchi Sugihiro、Kikuchi Mihoko、Katoh Shungo、Anh Le Kim、Anh Nguyen Thi Hien、Anh Dang Duc、Smith Chris、Maruyama Haruhiko、Yoshida Lay-Myint、Cuong Do Duy、Thuy Pham Thanh、Ariyoshi Koya
  • Journal Title: PLOS Neglected Tropical Diseases Volume: 14 Issue: 12 Pages: 0008937-0008937
  • DOI: 10.1371/journal.pntd.0008937
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An outbreak of Paragonimus westermani infection among Cambodian technical intern trainees in Japan, exhibiting various extrapulmonary lesions. (2020)
  • Author(s): Nakagawa Y, Ikematsu Y, Nakanishi T, Ogawa Y, Taen R, Nakashima Y, Okabe H, Yoshida A, Maruyama H
  • Journal Title: Parasitology International Volume: 81 Pages: 102279-102279
  • DOI: 10.1016/j.parint.2020.102279
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of a serodiagnosis system for the detection of Toxocara spp. and Ascaris suum infection in chickens. (2020)
  • Author(s): Nguyen YTH, Hayata Y, Sonoda S, Nonaka N, Maruyama H, Yoshida A.
  • Journal Title: Parasitol Int. Volume: 75 Pages: 102022-102022
  • DOI: 10.1016/j.parint.2019.102022
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Human proliferative sparganosis update. (2020)
  • Author(s): Kikuchi T, Maruyama H
  • Journal Title: Parasitol Int. Volume: 75 Pages: 102036-102036
  • DOI: 10.1016/j.parint.2019.102036
  • Peer Reviewed
• [Journal Article] 1.Phylogenetic relationships of Strongyloides species in carnivore hosts. (2020)
  • Author(s): Phoo Pwint Ko, Kazuo Suzuki, Marco Canales-Ramos, Myo Pa Pa Thet Hnin Htwe Aung, Wah Win Htike, Ayako Yoshida, Martin Montes, Kazuhiro Morishita, Eduardo Gotuzzo, Haruhiko Maruyama, Eiji Nagayasu.
  • Journal Title: Parasitol Int. Volume: 78 Pages: 102151-102151
  • DOI: 10.1016/j.parint.2020.102151
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Paragonimus and paragonimiasis in Asia: An update. (2019)
  • Author(s): Yoshida A, Doanh PN, Maruyama H
  • Journal Title: Acta Trop. Volume: 199 Pages: 105074-105074
  • DOI: 10.1016/j.actatropica.2019.105074
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] First molecular identification of Strongyloides vituli in cattle in Japan and insights into the evolutionary history of Strongyloides parasites of ruminants. (2019)
  • Author(s): Ko PP, Sakaguchi K, Yoshida A, Maruyama H, Nonaka N, Nagayasu E.
  • Journal Title: Parasitol Int. Volume: 72 Pages: 101937-101937
  • DOI: 10.1016/j.parint.2019.101937
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 日本における寄生虫症の動向と免疫診断について (2019)
  • Author(s): 丸山治彦
  • Organizer: 第30回 日本臨床微生物学会
  • Invited
• [Presentation] 寄生虫症抗体検査のこれまでとこれから (2019)
  • Author(s): 丸山治彦
  • Organizer: 第30回日本臨床寄生虫学会
  • Invited
• [Presentation] 日本における寄生虫症の動向と免疫診断について (2019)
  • Author(s): 丸山治彦
  • Organizer: 第30回日本臨床微生物学会学術集会
  • Invited
• [Presentation] 現在のわが国における寄生蠕虫症の症状について (2018)
  • Author(s): 丸山治彦、鴻巣明日香、阿部愛、村瀬一典、菊地泰生、長安英治
  • Organizer: 第29回日本臨床寄生虫学会
• [Presentation] Paragonimiasis among Asian immigrants in Japan (2018)
  • Author(s): Haruhiko Maruyama, Eiji Nagayasu, Ayako Yoshida
  • Organizer: 14th International Congress of Parasitology
  • Int'l Joint Research / Invited