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Translocation pathway of the bacterial flagellar typeIII secretion system analyzed by site-directed photo-crosslinking

Research Project

Project/Area Number 18K07108
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 49050:Bacteriology-related
Research InstitutionNagasaki University (2020)
Nagoya University (2018-2019)

Principal Investigator

TERASHIMA Hiroyuki  長崎大学, 熱帯医学研究所, 助教 (60791788)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords細菌べん毛 / III型分泌装置 / 光架橋 / in vitro再構成 / タンパク質分泌 / 輸送装置 / タンパク質輸送 / 反転膜
Outline of Final Research Achievements

We studied the mechanism of protein export, construction and function of the bacterial flagellum. Using an in vitro transport assay with the inverted membrane vesicles, we were able to construct hook and filament in vitro. The length of hook was regulated to 55 nm by the molecular ruler protein FliK. These results revealed that the flagellum is a molecular machine that is autonomously constructed using only flagellar components.

Academic Significance and Societal Importance of the Research Achievements

細菌べん毛は、細胞外に長く伸びた線維状の超分子複合体である。その構築メカニズムを明らかにすることによって、タンパク質から構築される分子機械の作動原理と設計原理を知ることができる。本研究の成果によって、べん毛の構築原理の一端を解き明かすことができ、学術的意義のある研究成果を得ることができた。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (12 results)

All 2021 2020 2019 2018

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 4 results) Presentation (6 results) (of which Invited: 2 results)

  • [Journal Article] Site-directed crosslinking identifies the stator-rotor interaction surfaces in a hybrid bacterial flagellar motor2021

    • Author(s)
      Terashima Hiroyuki、Kojima Seiji、Homma Michio
    • Journal Title

      Journal of Bacteriology

      Volume: 9 Issue: 9

    • DOI

      10.1128/jb.00016-21

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assembly mechanism of a supramolecular MS-ring complex to initiate bacterial flagellar biogenesis in Vibrio species.2020

    • Author(s)
      Terashima H, Hirano K, Inoue Y, Tokano T, Kawamoto A, Kato T, Yamaguchi E, Namba K, Uchihashi T, Kojima S, Homma M.
    • Journal Title

      Journal of Bacteriology

      Volume: - Issue: 16

    • DOI

      10.1128/jb.00236-20

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In Vitro Autonomous Construction of the Flagellar Axial Structure in Inverted Membrane Vesicles.2020

    • Author(s)
      Terashima H, Tatsumi C, Kawamoto A, Namba K, Minamino T, Imada K.
    • Journal Title

      Biomolecules

      Volume: 10 Issue: 1 Pages: 126-126

    • DOI

      10.3390/biom10010126

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure of Vibrio FliL, a New Stomatin-like Protein That Assists the Bacterial Flagellar Motor Function2019

    • Author(s)
      Norihiro Takekawa, Miyu Isumi, Hiroyuki Terashima, Shiwei Zhu, Yuuki Nishino, Mayuko Sakuma, Seiji Kojima, Michio Homma, Katsumi Imada
    • Journal Title

      mBio

      Volume: 10(2) Issue: 2

    • DOI

      10.1128/mbio.00292-19

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] In Vitro Reconstitution of Functional Type III Protein Export and Insights into Flagellar Assembly2018

    • Author(s)
      Hiroyuki Terashima, Akihiro Kawamoto, Chinatsu Tatsumi, Keiichi Namba, Tohru Minamino, Katsumi Imada
    • Journal Title

      mBio

      Volume: 9(3) Issue: 3

    • DOI

      10.1128/mbio.00988-18

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel insight into an energy transduction mechanism of the bacterial flagellar type III protein export2018

    • Author(s)
      Hiroyuki Terashima, Katsumi Imada
    • Journal Title

      Biophysics and Physicobiology

      Volume: 15 Issue: 0 Pages: 173-178

    • DOI

      10.2142/biophysico.15.0_173

    • NAID

      130007437793

    • ISSN
      2189-4779
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 細菌べん毛回転に必須な固定子‐回転子間の相互作用機序2021

    • Author(s)
      〇寺島浩行、小嶋誠司、本間道夫
    • Organizer
      第94回日本細菌学会総会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] In vitro分泌再構成系を用いたべん毛III型分泌装置の作動メカニズムの解明2020

    • Author(s)
      〇寺島 浩行、川本 晃大、巽 千夏、難波 啓一、南野 徹、本間 道夫、今田 勝巳
    • Organizer
      第34回日本バイオフィルム学会学術集会&第57回日本細菌学会中部支部総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 部位特異的架橋法による細菌べん毛固定子-回転子間相互作用の検出2020

    • Author(s)
      〇寺島浩行、小嶋誠司、本間道夫
    • Organizer
      日本生体エネルギー研究会 第46回討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 網羅的光架橋法による細菌べん毛回転モーターの固定子ー回転子間相互作用の解析2020

    • Author(s)
      〇寺島浩行、小嶋誠司、本間道夫
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 細菌III型分泌装置に対する阻害剤を探索だい929するための新規網羅的アッセイ系2019

    • Author(s)
      〇寺島浩行、川本晃大、巽千夏、難波啓一、南野徹、本間道夫、今田勝巳
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 細菌べん毛輸送装置によるタンパク質輸送の反転膜小胞を用いたin vitro再構成2019

    • Author(s)
      ○寺島浩行、川本晃大、巽千夏、難波啓一、南野徹、今田勝巳
    • Organizer
      第83回日本生化学会中部支部例会
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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