Analysis of the regulatory mechanism of virulence expression by the retron containing reverse transcriptase gene in Vibrio cholerae
| Project/Area Number |
18K07113
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 49050:Bacteriology-related
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
成谷 宏文 十文字学園女子大学, 人間生活学部, 教授 (30452668)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | レトロン / 逆転写酵素 / msDNA / Vibrio cholerae / Vibrio mimicus / コレラ菌 / arcAB / arcA / arcB / デコイ核酸 / 可動性遺伝因子 |
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| Outline of Final Research Achievements |
Retron-Vc95, which contains the reverse transcriptase gene (ret) of Vibrio cholerae, has been suggested to be associated with virulence expression. In order to easily monitor the expression of retron, a reporter strain with the β-glucuronidase gene (gusA) inserted under the promoter of retron was created, and the expression of retron was analyzed under various culture conditions. The expression of retron was found to be decreased under various stress conditions, such as the addition of NaCl and iron chelators, and the deficiency of nitrogen and carbon sources.
In addition, the structure of retron-Vm85 in V. mimicus, a bacterium of the same genus as V. cholerae, was clarified, suggesting the possibility of horizontal transmission of retron.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は研究代表者が始めた病原細菌における逆転写酵素とmsDNAに関する研究成果に基づくものであり，独創的なものである。機能が明らかにされていなかった逆転写酵素とmsDNAおよびレトロンについて，病原細菌を研究している他の研究者はほとんど注目していなかった。本研究によって得られる成果は，病原性発現ネットワークの新たな分子メカニズムを解明する可能性を持っている。また，代表者のmsDNAデコイ核酸仮説（DNAアプタマー仮説）が実証されれば，遺伝子の転写制御研究という基礎的な分野でも，これまでに報告されていない新しい転写制御形式を提唱するものであり各研究分野に与えるインパクトは大きい。
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Report
(3 results)
Research Products
(6 results)
