Identification of long non-coding RNAs that regulate HBV replication
| Project/Area Number |
18K07161
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 49060:Virology-related
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| Research Institution | Fujita Health University (2020)
National Center for Global Health and Medicine (2018-2019) |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | HBV / lncRNA / HBV Core / HBV X / long non-coding RNA |
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| Outline of Final Research Achievements |
In this study, I investigated the roles of long non-coding RNA in HBV replication. HepG2 cells were expressed with HA- and myc-tagged HBV X protein. Cell lysates were immunoprecipitated with anti-HA and anti-myc antibodies. Immunocomplex were subjected to next generation sequence to identify lncRNAs bound to HBV X protein. I found that miRNA acts as suppressor of HBV replication through degradation of HBV X protein.
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| Academic Significance and Societal Importance of the Research Achievements |
今回、Xタンパク質と結合するmiRNAを同定した。この同定したmiRNAはHBV Xタンパク質と結合し、Xタンパク質の機能を阻害した。さらにこのmiRNAはHBV感染も阻害した。このmiRNAはXタンパク質と結合することにより、Xタンパク質を不安定化した。Xタンパク質は、HBVの転写を制御していることから、このmiRNAがHBVの潜伏化に関与している可能が示唆される。また、このmiRNAを人工的に制御できれば、HBV阻害薬のターゲットととして期待される。
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Report
(4 results)
Research Products
(9 results)
