Study of new boron carriers using carbon nano horns

• Project/Area Number: 18K07705
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 52040:Radiological sciences-related
• Research Institution: University of Tsukuba
• Principal Investigator: Yoshida Fumiyo 筑波大学, 医学医療系, 研究員 (30261811)
• Co-Investigator(Kenkyū-buntansha): 中井 啓 筑波大学, 医学医療系, 准教授 (50436284) ; 湯田坂 雅子 国立研究開発法人産業技術総合研究所, 材料・化学領域, 招聘研究員 (70159226) ; 岡崎 俊也 国立研究開発法人産業技術総合研究所, 材料・化学領域, 副研究センター長 (90314054) ; 白川 真 福山大学, 薬学部, 講師 (40707759) ; ザボロノク アレクサンドル 筑波大学, 医学医療系, 助教 (20723117) ; 鶴淵 隆夫 筑波大学, 医学医療系, 講師 (70778901)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: BN-CNH / BNCT / カーボンナノホーン

Outline of Final Research Achievements

Carbon nanohorns (CNH) are pseudo-cylindrical materials made of a single piece of graphite with a diameter of 2-5 nm and a length of 40-50 nm. Boron-carbon nanohorns (BN-CNH) are nanoparticles that can be used as a new boron compound, containing a large amount of boron within CNH and also connected to the outer wall of the particles. To evaluate BN-CNH, boron uptake experiments using cell cultures, colony-forming assay after neutron irradiation, in vivo boron distribution after mouse tail vein and topical administration were performed. The effect of BN-CNH was limited compared to the positive control using BPA.

Academic Significance and Societal Importance of the Research Achievements

BN-CNHは全身投与の場合大半が脾臓にトラップされてしまい、局所投与では、拡散しないためむらが生じる等の問題がある。また、培養細胞を用いた中性子照射実験では、BPAに比べ1／5程度の効果しか得られなかった。そのためそのままで臨床に供することは難しいが、その一因としてホウ素源材料に10Bが1／5しか含まれないナチュラルボロンを使用していることが考えられる。ホウ素源としてB10を使用し、局所投与の方法を検討することにより、新しいホウ素薬剤となる可能性があると考えられる。

Research Products

• [Journal Article] Optimization of preparation methods for high loading content and high encapsulation efficiency of BSH into liposomes (2021)
  • Author(s): Shirakawa Makoto、Nakai Kei、Sato Yuhki、Nakamura Shunji、Harada Mari、Ishihara Kazuki、Yoshida Fumiyo、Matsumura Akira、Tomida Hisao
  • Journal Title: Applied Radiation and Isotopes Volume: 169 Pages: 109260-109260
  • DOI: 10.1016/j.apradiso.2020.109260
  • Peer Reviewed
• [Journal Article] Boron analysis and imaging of cells with 2-hr BPA exposure by using micro-proton particle-induced gamma-ray emission (PIGE) (2020)
  • Author(s): Nakai Kei、Endo Keita、Yoshida Fumiyo、Koka Masashi、Yamada Naoto、Satoh Takahiro、Tsurubuchi Takao、Matsumura Akira、Matsumoto Yoshitaka、Sakurai Hideyuki
  • Journal Title: Applied Radiation and Isotopes Volume: 165 Pages: 109334-109334
  • DOI: 10.1016/j.apradiso.2020.109334
  • Peer Reviewed
• [Journal Article] Difference in BPA uptake between glioma stem-like cells and their cancerous cells (2020)
  • Author(s): Yoshida Fumiyo、Kurita Tadashi、Endo Keita、Nakai Kei、Shirakawa Makoto、Zaboronok Alexander、Tsurubuchi Takao、Ishikawa Eiichi、Matsumura Akira
  • Journal Title: Applied Radiation and Isotopes Volume: 164 Pages: 109234-109234
  • DOI: 10.1016/j.apradiso.2020.109234
  • Peer Reviewed
• [Journal Article] Evaluation of a Novel Boron-Containing α-d-Mannopyranoside for BNCT (2020)
  • Author(s): Tsurubuchi Takao、Shirakawa Makoto、Kurosawa Wataru、Matsumoto Kayo、Ubagai Risa、Umishio Hiroshi、Suga Yasuyo、Yamazaki Junko、Arakawa Akihiro、Maruyama Yutaka、Seki Takuya、Shibui Yusuke、Yoshida Fumiyo、Zaboronok Alexander、Suzuki Minoru、Sakurai Yoshinori、Tanaka Hiroki、Nakai Kei、Ishikawa Eiichi、Matsumura Akira
  • Journal Title: Cells Volume: 9 Issue: 5 Pages: 1277-1277
  • DOI: 10.3390/cells9051277
  • Peer Reviewed
• [Presentation] Boron analysis and imaging of 2hr-BPA-exposured cells by using micro proton particle induced gamma-ray emission (PIGE). (2018)
  • Author(s): Kei Nakai, Keita Endo, Fumiyo Yoshida, Masashi Koka, Naoto Yamada, Takahiro Satoh, Akira Matsumura
  • Organizer: International Congress on Neutron Capture Therapy
  • Int'l Joint Research
• [Presentation] Hybrid gold and boron nanoparticles for treatment and boron dose estimation in boron neutron capture therapy for malignant glioma (2018)
  • Author(s): Alexander Zaboronok, Sergey Taskaev, Vladimir Kanygin, Timofei Bykov, Kazutaka Mitsuishi, Eisuke Sato, Kei Nakai,Takao Tsurubuchi, Fumiyo Yoshida, Eiichi Ishikawa, Bryan J. Mathis, Tetsuya Yamamoto,Akira Matsumura
  • Organizer: International Congress on Neutron Capture Therapy
  • Int'l Joint Research